S-RNase based S-genotyping of Croatian sweet cherry (Prunus avium L.) genotypes

Erċışlı, Sezai; Radunić, Mira; Gadže, Jelena; İpek, Ahmet; Škaljac, Marisa; Čmelik, Zlatko

doi:10.1016/j.scienta.2012.02.041

Cited by 12 publications

(25 citation statements)

References 23 publications

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“…Following the use of the PaConsIF/PaConsIR primers, no product of the amplification of the S 13 allele was obtained, like in the analyses by Sonneveld et al (2003) and Schuster et al (2007). After amplifying intron I, it was impossible to distinguish the alleles S 4 from S 6 , S 1 from S 5 , and S 2 from S 9 , which confirms the results obtained for sweet cherry cultivars from Turkey and Croatia (Ipek et al 2011;Ercisli et al 2012). Amplification of intron II did not make it possible to identify the alleles S 1 , S 3 and S 13 , which is consistent with the results obtained by other authors Schuster et al 2007;Ipek et al 2011).…”

Section: Discussionsupporting

confidence: 78%

“…Specific primers based on the S-RNase gene were developed for the alleles S 1 -S 16 (Sonneveld et al 2001, and for alleles S 17 -S 19 , S 21 -S 25 , S 34 and S 37 (Szikriszt et al 2013). The effectiveness of the use of consensus primers and specific primers in the identification of the S-alleles of sweet cherry was confirmed in the studies by Békefi et al (2003); De Cuyper et al (2005); Schuster et al (2007); Ipek et al (2011);Ercisli et al (2012); Szikriszt et al (2013).…”

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confidence: 91%

“…Identification of S-alleles was performed for sweet cherry cultivars from Western Europe, for Belgian and British wild cherries, for sweet cherry cultivars and selections gathered in the Swedish gene bank, and for the local Sicilian, Spanish, Greek, Turkish and Croatian cultivars Tobutt et al 2004;De Cuyper et al 2005;Marchese et al 2007;Gisbert et al 2008;Lacis et al 2008;Vaughan et al 2008;Ganopoulos et al 2010;Ipek et al 2011;Ercisli et al 2012;Szikriszt et al 2013;Cachi, Wünsch 2014). S-alleles were also identified in sweet cherry cultivars originating in several countries of Central and Eastern Europe, mostly in the cultivars and selections gathered in the Latvian collection, and also in the Hungarian cultivars, but in only a few of the Czech and Ukrainian cultivars (Békefi et al 2003(Békefi et al , 2010Schuster et al 2007;Lacis et al 2008;Schuster 2012).…”

mentioning

confidence: 99%

“…However, there is still no information on the composition of S-alleles for a large group of sweet cherry cultivars from the central and eastern regions of Europe, particularly from Ukraine. Since the existing results indicate an uneven distribution of S-alleles in the European cultivars of sweet cherry (Ercisli et al 2012), identification of S-alleles in the cultivars from Central and Eastern Europe will help expand our knowledge on this subject.…”

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confidence: 99%

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Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe

Lisek

Rozpara

Głowacka

et al. 2015

Hort. Sci. (Prague)

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Lisek A., Rozpara E., Głowacka A., Kucharska D., Zawadzka M. (2015): Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe. Hort. Sci. (Prague), 42: 13-21.Sweet cherry is a species that is characterized by self-incompatibility, which causes difficulties in obtaining high yields of sweet cherry fruit. Determination of the composition of the S-alleles of sweet cherry cultivars is useful both to growers producing the fruit and breeders when selecting cultivars for cross-fertilizations. In this work, S-alleles have been identified for 47 sweet cherry cultivars from Central and Eastern Europe, mostly from Ukraine and the Czech Republic, with S-genotypes of 43 cultivars identified for the first time. To identify the S-alleles, PCR-based methods were used. In each cultivar, two of the following eight S-alleles were identified: S 1 , S 2 , S 3 , S 4 , S 5 , S 6 , S 9 and S 13 . In the cultivars from the Czech Republic, the most frequently occurring were the alleles S 3 , S 1 and S 4 . In the cultivars originating in Ukraine, the most frequently found were the alleles S 5 and S 9 , which makes them differ considerably from the sweet cherry cultivars from other regions of Europe. The tested sweet cherry cultivars were assigned to 20 of the existing incompatibility groups.

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Section: Discussionsupporting

confidence: 78%

mentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe

Lisek

Rozpara

Głowacka

et al. 2015

Hort. Sci. (Prague)

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“…Ercisli et al (2012) utilizaram o par de iniciadores específicos PaConsI/PaConsII e obtiveram sucesso na amplificação dos dois alelos-S em cultivares de cereja (P. avium), o que mostra a precisão na utilização de iniciadores específicos. Entretanto, esta especificidade poderia torná-los ineficientes para análise de outras cultivares, o que limitaria a identificação de apenas uma ou poucas cultivares.…”

Section: Resultsunclassified

Identificação por PCR dos alelos-S associados à compatibilidade gametofítica em ameixeira japonesa

Conti

Carmo

Raseira

et al. 2013

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi identificar e caracterizar molecularmente os alelos-S de cultivares de ameixeira japonesa e verificar a compatibilidade gametofítica entre estes. Foram utilizados dois pares de iniciadores específicos na amplificação dos alelos via PCR, em 18 cultivares: Pluma 7, Gulf Rubi, Blood Plum, Wickson, América, Santa Rosa, Rosa Mineira, Estrela Púrpura, Amarelinha, The First, Harry Pieckstone, Santa Rita, Seleção 16, Seleção A26 (Burbank), Seleção 21, Seleção 19, Methley e Simka. As condições da PCR e as combinações de oligonucleotídeos iniciadores utilizadas permitiram a identificação de alelos-S nas cultivares, bem como a indicação dos polinizadores mais compatíveis. As cultivares América e Santa Rosa, bem como Blood Plum, Wickson, Rosa Mineira, Estrela Púrpura e Seleção 21 apresentam incompatibilidade total entre si, por compartilharem os mesmos alelos.Termos para indexação: Prunus salicina, autoincompatibilidade gametofítica, S-RNase. Identification by PCR of S-alleles associated with gametophytic compatibility in Japanese plumAbstract -The objective of this work was to molecularly identify and characterize the S-alleles of Japanese plum cultivars and to verify the gametophytic compatibility between them. Two pairs of specific primers were used for allele amplification by PCR in 18 cultivars: Pluma 7, Gulf Rubi, Blood Plum, Wickson, América, Santa Rosa, Rosa Mineira, Estrela Púrpura, Amarelinha, The First, Harry Pieckstone, Santa Rita, Seleção 16, Seleção A26 (Burbank), Seleção 21, Seleção 19, Methley, and Simka. PCR conditions and oligonucleotide primer combinations used allowed the identification of S-alleles in cultivars, as well as the indication of the most compatible pollinators. América and Santa Rosa cultivars, as well as Blood Plum, Wickson, Rosa Mineira, Estrela Púrpura, and Seleção 21 show total incompatibility among each other, since they share the same alleles.

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Genomics Opportunities and Breeding Strategies Towards Improvement of Climate-Smart Traits and Disease Resistance Against Pathogens in Sweet Cherry

Zambounis

Ganopoulos

Aravanopoulos

et al. 2020

Genomic Designing of Climate-Smart Fruit Crops

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S-RNase based S-genotyping of Croatian sweet cherry (Prunus avium L.) genotypes

Cited by 12 publications

References 23 publications

Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe

Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe

Identificação por PCR dos alelos-S associados à compatibilidade gametofítica em ameixeira japonesa

Genomics Opportunities and Breeding Strategies Towards Improvement of Climate-Smart Traits and Disease Resistance Against Pathogens in Sweet Cherry

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