S100 and p65 expression are increased in the masseter muscle after botulinum toxin-A injection

Park, Young-Wook; Kim, Seong‐Gon; Jo, You‐Young

doi:10.1186/s40902-016-0079-8

Cited by 8 publications

(3 citation statements)

References 32 publications

(35 reference statements)

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“…Subgroup IVB also showed a significant increase in the area percentage of anti-S100 protein when compared to other BTX-A injected subgroups. This agrees with the study done by Park et al, 2016 [39] who examined the effect of 5 u and 10 u of BTX-A injection on the masseter muscle of rats, where expression of S100 protein was increased in the masseter muscle of BTX-A injected groups when compared to the control. The authors postulated the increase in S100 protein might be due to muscle atrophy or apoptotic stress after injection with BTX-A.…”

Section: Discussionsupporting

confidence: 92%

Influence of Intramasseteric Injection of Botulinum Toxin Type A on the Ipsilateral Submandibular Salivary Gland of Albino Rats

Hussein

2020

Egyptian Journal of Histology

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Background: Botulinum toxins are used in treating involuntary muscle contraction such as hemifacial spasm and facial nerve palsy, hyperhidrosis or excessive saliva production. Minor side effects are reported like local hematoma, pain, dry mouth and viscous saliva. However; major complications may occur such as loss of motor head control and aspiration pneumonia. Some animal studies showed minimal histological changes. While, some authors have demonstrated significant structural and functional changes in the parotid gland of rats and the submandibular gland of rabbits after being injected with botulinum toxin type-A (BTX-A). Aim of the Study:To evaluate the effect of intramasseteric injection of BTX-A on the ipsilateral submandibular gland in Albino rats. Materials and Methods: Fifty six adult male Albino rats were divided into four groups. Rats of group (I) and group (II) received intramasseteric injection of 0.05 ml or 0.1 ml of 0.9% sodium chloride solution respectively. Rats of group (III) and group (IV) received intra-masseteric injection of 2.5 units or 5 units of BTX-A respectively. Each group was further subdivided into two subgroups according to the time of sacrifice which was at three or fourteen days. Sections of glands were stained by H&E and S100 protein antibody. Results: Glands' acini of group III and IV showed cytoplasmic vacuolations. Acini of group IV at fourteen days showed irregular outline with areas of complete degeneration. Duct system of both groups showed signs of degeneration. Using immunohistochemical staining, group III and IV showed increased area percentage of positive reaction than control groups. Statistical analysis of acini diameter and area percentage of anti-S100 protein revealed significant increase in botox injected subgroups. Conclusion: Intramasseteric injection with BTX-A was able to induce histological changes in submandibular gland of the ipsilateral side.

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Section: Discussionsupporting

confidence: 92%

Influence of Intramasseteric Injection of Botulinum Toxin Type A on the Ipsilateral Submandibular Salivary Gland of Albino Rats

Hussein

2020

Egyptian Journal of Histology

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“…Paralysis of the muscle contributes to bone degradation and changes in the bone morphology [ 30 ]. When BTX-A is injected into the masseter muscle, the mandibular bone mineral content is significantly reduced [ 21 ], and the thickness of the mandible ramus is also decreased with masseter muscle atrophy [ 32 ]. Paralysis of the masseter and temporalis muscles induces bone loss in the alveolar and condylar areas [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of the masseter muscle injection of botulinum toxin A on the mandibular bone growth of developmental rats

Seok

Kim

et al. 2018

Maxillofac Plast Reconstr Surg

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BackgroundThe objective of this study was to evaluate the influence of masticatory muscle injection of botulinum toxin type A (BTX-A) on the growth of the mandibular bone in vivo.MethodsEleven Sprague-Dawley rats were used, and BTX-A (n = 6) or saline (n = 5) was injected at 13 days of age. All injections were given to the right masseter muscle, and the BTX-A dose was 0.5 units. All of the rats were euthanized at 60 days of age. The skulls of the rats were separated and fixed with 10% formalin for micro-computed tomography (micro-CT) analysis.ResultsThe anthropometric analysis found that the ramus heights and bigonial widths of the BTX-A-injected group were significantly smaller than those of the saline-injected group (P < 0.05), and the mandibular plane angle of the BTX-A-injected group was significantly greater than in the saline-injected group (P < 0.001). In the BTX-A-injected group, the ramus heights II and III and the mandibular plane angles I and II showed significant differences between the injected and non-injected sides (P < 0.05). The BTX-A-injected side of the mandible in the masseter group showed significantly lower mandibular bone growth compared with the non-injected side.ConclusionBTX-A injection into the masseter muscle influences mandibular bone growth.

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“…The paralytic effect of BTX in masseter muscle influences the structural changes of mandible in rat [ 19 ]. The BTX-injected side of the mandible shows the significantly reduced bone mineral content and cortical bone thickness [ 19 , 52 ], and the proportion of the trabecular bone area is also reduced [ 19 ]. The rats that were BTX-injected in both masseter and temporalis show significantly reduced the trabecular bone in the alveolar and condylar bone region [ 53 ].…”

Section: Broken Balance Muscle Function By Btx Injection and Its Ementioning

confidence: 99%

Correction of Malocclusion by Botulinum Neurotoxin Injection into Masticatory Muscles

Seok

Kim

2018

Toxins

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Botulinum toxin (BTX) is a neurotoxin, and its injection in masticatory muscles induces muscle weakness and paralysis. This paralytic effect of BTX induces growth retardation of the maxillofacial bones, changes in dental eruption and occlusion state, and facial asymmetry. Using masticatory muscle paralysis and its effect via BTX, BTX can be used for the correction of malocclusion after orthognathic surgery and mandible fracture. The paralysis of specific masticatory muscles by BTX injection reduces the tensional force to the mandible and prevents relapse and changes in dental occlusion. BTX injection in the anterior belly of digastric and mylohyoid muscle prevents the open-bite and deep bite of dental occlusion and contributes to mandible stability after orthognathic surgery. The effect of BTX injection in masticatory muscles for maxillofacial bone growth and dental occlusion is reviewed in this article. The clinical application of BTX is also discussed for the correction of dental malocclusion and suppression of post-operative relapse after mandibular surgery.Keywords: botulinum neurotoxin; masticatory system; maxillofacial bone; dental occlusion; orthognathic surgery Key contribution: BTX injection into masticatory muscles affects the maxillofacial bony growth and dental occlusion. In clinical practices, BTX injection has been used for reducing post-operative relapse after mandibular surgery.

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S100 and p65 expression are increased in the masseter muscle after botulinum toxin-A injection

Cited by 8 publications

References 32 publications

Influence of Intramasseteric Injection of Botulinum Toxin Type A on the Ipsilateral Submandibular Salivary Gland of Albino Rats

Influence of Intramasseteric Injection of Botulinum Toxin Type A on the Ipsilateral Submandibular Salivary Gland of Albino Rats

Effect of the masseter muscle injection of botulinum toxin A on the mandibular bone growth of developmental rats

Correction of Malocclusion by Botulinum Neurotoxin Injection into Masticatory Muscles

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