S100 calcium-binding protein A10 contributes to malignant traits in osteosarcoma cells by regulating glycolytic metabolism via the AKT/mTOR pathway

Abstract

As an aggressive musculoskeletal malignancy, osteosarcoma (OSa) is popular among young adults and teenagers worldwide. S100 calcium-binding protein A10 (S100A10) functioned as a novel tumor-promoting protein in several human cancers. However, its role in OSa remains obscure. In this study, gene and protein levels were respectively determined by RT-qPCR or Western blotting. OSa cell proliferation, apoptosis, and metastasis were evaluated via CCK-8, colony formation, flow cytometry, and Transwell assays. To assess the glycolysis level, glucose consumption and lactate production were detected. It was found S100A10 was highly expressed in OSa tissues and cell lines. Besides, S100A10 facilitated proliferation and metastasis, and inhibited apoptosis in OSa cells. In addition, S100A10 regulated OSa cell proliferation, metastasis and apoptosis via mediating the glycolysis process. Furthermore, S100A10-mediated AKT/mTOR signaling accelerated glycolysis, thereby promoting malignant behaviors in OSa cells. Taken together, our findings indicated that S100A10 might promote malignant phenotypes of OSa cells by accelerating glycolysis and activating the AKT/mTOR signaling, providing a promising target for OSa treatment.

S100A10 Promotes Pancreatic Ductal Adenocarcinoma Cells Proliferation, Migration and Adhesion through JNK/LAMB3-LAMC2 Axis

“…In hepatocellular carcinoma, the over-expression of S100A10 accelerates cell proliferation in Hep3B and Huh-7 cells, while decreased S100A10 expression shows the opposite effect on the proliferation capacity of SK-Hep-1 and HepG2 cells [11]. Additionally, it is demonstrated that S100A10 promotes the malignant growth of cancer cells by activating the AKT/mTOR signaling pathway in osteosarcoma [21] and the Src/ANXA2/AKT/mTOR signaling pathway in gastric cancer [19]. Collectively, S100A10 serves as a positive regulator in cell proliferation in multiple cancers.…”

Reprogramming of glucose metabolism: Metabolic alterations in the progression of osteosarcoma

“…Research [111] has found that it is possible to promote aerobic glycolysis of OS cells by activating the PI3K/AKT/mTOR/c Myc pathway. S100 calcium binding protein A10 (S100A10) is another protein molecule overexpressed in OS tissue, which can accelerate the glycolysis process of OS by activating AKT/mTOR signaling, thereby promoting the malignant transformation of OS cells [112] . This suggests that the PI3K/Akt/mTOR signaling pathway may be involved in the glycolysis process of OS, thereby accelerating the proliferation, migration, and invasion of OS cells.…”

An in vitro model for Reactive Astrogliosis: Differentiated U-87 MG cells as biochemical analogues of reactive astrocytes

“…On contrary, the characteristics of A2 were partially con rmed by enhanced expression of A2 speci c Ca 2+ binding protein i.e. S100A10, knockout of which might lead to inhibited proliferation, migration, invasion and increased apoptosis [37]. Thus, S100A10 contributes towards sustainability of astrocytes in addition to inducing neurite outgrowth [38] .…”