S100 calcium‐binding protein A12 as a diagnostic index for subclinical mastitis in cows

Abstract

Diagnosis of subclinical mastitis is very important in management of the dairy industry and improvement of dairy cow productivity. S100A12, that is found in related tissues of mammals, is considered as an index for diagnosing inflammatory reaction. To evaluate whether S100A12 is involved in subclinical mastitis, milk somatic cell mRNA from 276 dairy cows was used to detect the transcriptional level of S100A12 by real-time quantitative polymerase chain reaction. A predictive analysis for mastitis was performed, and the correlation between S100A12 and other subclinical mastitis indicators was also assessed. The transcriptional levels of S100A12 in the milk of cows with mastitis were significantly higher than those in the milk of healthy cows (p < 0.05). The correlation analysis showed that S100A12 was positively associated with the somatic cell count and the sodium and chloride concentrations of milk. In contrast, a negative correlation was found between S100A12 and the potassium concentration and pH of milk. However, no significant correlation was detected between S100A12 and the other parameters, such as protein, lactose, ash, fat, density, Ca and SNF. These results suggested that the S100A12 level in milk may serve as a diagnostic tool for subclinical mastitis in cows without obvious clinical signs.

Multi-Tissue Transcriptome Study of Innate Immune Gene Expression Profiling Reveals Negative Energy Balance Altered the Defense and Promoted System Inflammation of Dairy Cows

“…It recruits leukocytes, elevates cytokines and chemokines, and controls leukocyte adhesion and migration [ 73 ]. In cows, S100A12 is a marker for inflammatory responses and subclinical mastitis [ 74 ]. The upregulation of LBP, PTX3, and S100A12 in multiple tissues suggests that NEB influences systemic inflammation, and these three genes may serve as diagnostic indicators of systemic inflammation caused by NEB.CXCL2 regulates and inflames inflammation [ 75 ].…”

Beneficial Effects of Lactobacillus Zeae on the Gut Health of Piglets Challenged with Enterotoxigenic Escherichia Coli

“…IL-8 can bind to CXCR1 and CXCR2 receptors with high a nity, and as the primary chemokine of neutrophils, it plays a vital role in the initiation and maintenance of in ammatory response [39]. S100A12 found in mammalian tissues is considered to be an indicator for the diagnosis of in ammatory responses [40]. S100A12 is associated with innate immune responses and is mainly expressed in neutrophils and monocytes.…”

Relationship between blood calcium level and post-milking teat canal closure in dairy cows

“…Subclinical hypocalcemia impairs the metabolic and physiological responses that regulate the welfare and productivity of transitional dairy cows (Rodrigues et al, 2020). Calcium mineral is crucial in numerous structural and biochemical processes in udder defense mechanisms, including the contractile activity of the teat sphincter (Paulrud, 2005;Mahjoubi et al, 2017), immune cell functions (Kimura et al, 2006;Zhang et al, 2019), blood coagulation (Libera et al, 2021), and teat canal keratin formation and S100 calcium-binding protein functions (Smolenski et al, 2015;Zhong et al, 2018). Histomorphological studies have shown that the teat canal returns to its original state after milking with the contraction of exible, smooth muscle cells surrounding the teat canal (Giesecke et al, 1972;Van Der Merwe, 1985;Paulrud, 2005).…”