S179D prolactin diminishes the effects of UV light on epidermal gamma delta T cells

Abstract

Epidermal gamma delta T cells (γδ T) and Langerhans cells (LC) are immune cells altered by exposure to ultraviolet radiation (UVB), a powerful stressor resulting in immune suppression. Prolactin (PRL) has been characterized as an immunomodulator, particularly during stress. In this study, we have asked whether separate administration of the two major forms of prolactin, unmodified and phosphorylated, to groups of 15 mice (3 experiments, each with 5 mice per treatment group) affected the number and morphology of these epidermal immune cells under control conditions, and following UV irradiation. Under control conditions, both PRLs reduced the number of γδ T, but a molecular mimic of phosphorylated PRL (S179D PRL) was more effective, resulting in a 30% reduction. In the irradiated group, however, S179D PRL was protective against a UV-induced reduction in γδ T number and change in morphology (halved the reduction and normalized the morphology). In addition, S179D PRL, but not unmodified (U-PRL), maintained a normal LC: γδ T ratio and sustained the dendritic morphology of LC after UV exposure. These findings suggest that S179D PRL may have an overall protective effect, countering UV-induced cellular alterations in the epidermis.

Técnicas De Análise Espacial Aplicadas Na Atenção Primária Em Saúde Em Fortaleza No Ceará: Estudo De Caso Da Dengue No Contexto Sócio Sanitário Da Pandemia Da Covid-19 Na Uaps Maurício Mattos Dourado

“…The action of PRL on the cells of the immune system was observed by Guzmán et al (2008) when they evaluated the protective effect of this hormone against the effects of exposure to UV light. C3H/ Hej mice received PRL and, after 10 days of treatment, were irradiated with UV light in a dose sufficient to induce an inflammatory response; 11 days after irradiation, skin samples were obtained for immunohistochemical analysis.…”