2022
DOI: 10.1021/acs.jpclett.2c01998
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S2 Subunit of SARS-CoV-2 Spike Protein Induces Domain Fusion in Natural Pulmonary Surfactant Monolayers

Abstract: Pulmonary surfactant has been attempted as a supportive therapy to treat COVID-19. Although it is mechanistically accepted that the fusion peptide in the S2 subunit of the S protein plays a predominant role in mediating viral fusion with the host cell membrane, it is still unknown how the S2 subunit interacts with the natural surfactant film. Using combined bio-physicochemical assays and atomic force microscopy imaging, it was found that the S2 subunit inhibited the biophysical properties of the surfactant and… Show more

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Cited by 4 publications
(2 citation statements)
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“…However, the involvement of structural rearrangements in the fusion process is supported by other studies. For example, Xu et al describe the interaction of the S2 subunit with the natural surfactant film on the host cell membrane, inducing microdomain fusion in the surfactant monolayer due to membrane fluidisation caused by the insertion of the S2 subunit, mediated by its fusion peptide [14]. Niort et al also elucidate that the repeated heptad domains of the S2 subunit bring the viral and cellular membranes close together while the fusion peptide interacts with and perturbs the membrane structure.…”
Section: Membrane Fusionmentioning
confidence: 99%
“…However, the involvement of structural rearrangements in the fusion process is supported by other studies. For example, Xu et al describe the interaction of the S2 subunit with the natural surfactant film on the host cell membrane, inducing microdomain fusion in the surfactant monolayer due to membrane fluidisation caused by the insertion of the S2 subunit, mediated by its fusion peptide [14]. Niort et al also elucidate that the repeated heptad domains of the S2 subunit bring the viral and cellular membranes close together while the fusion peptide interacts with and perturbs the membrane structure.…”
Section: Membrane Fusionmentioning
confidence: 99%
“…Most importantly, the CL-ADSA enables droplet oscillation to follow well-defined sinusoidal waveforms, thereby allowing for the study of interfacial dilational rheology, which provides novel implications about the viscoelastic properties and chemical composition of the interfacial monolayer . The CDS has evolved into a versatile experimental platform capable of various surfactant and lipid related studies, such as interfacial rheology, , surface thermodynamics,, ,, lipid–protein interactions, , toxicology of nanomaterials, , health impact of e-cigarette aerosols, , synthesis of biomaterials, and biophysical studies of tear films and xylem surfactants. , …”
Section: What Are the Techniques Used For Studying Pulmonary Surfactant?mentioning
confidence: 99%