S256. Long-Noncoding Rna Gas5 is Associated With Cocaine Action

Feng, Jian; Xu, Haiyang; Brown, Amber N.; Kaplan, Graham J.; Nestler, Eric J.

doi:10.1016/j.biopsych.2018.02.1148

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“…Long noncoding RNAs (lncRNAs) are widely identified as a class of noncoding RNAs which are more than 200 nucleotides (nt) (Feng, Xu, Brown, Kaplan, & Nestler, 2018). Emerging evidence has reported that lncRNAs are clearly associated with numerous pathological processes, including OA (Park, Lee, Chun, & Jin, 2017; Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Retracted: Long noncoding RNA CHRF exacerbates IL‐6‐induced inflammatory damages by downregulating microRNA‐146a in ATDC5 cells

Shi

et al. 2019

Journal Cellular Physiology

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Osteoarthritis (OA) is a frequent chronic musculoskeletal disorder which lacks applicably effective therapeutic strategy. In this study, we attempted to investigate whether long noncoding RNA (lncRNA) cardiac hypertrophy-related factor (CHRF) participated in mediating interleukin-6 (IL-6)-induced in vitro inflammatory damages as well as the regulatory mechanisms. ATDC5 cells were stimulated with IL-6, and then cellular damages were evaluated on the basis of cell viability by CCK-8, apoptotic cells by observation with flow cytometry, apoptosis-associated proteins by western blot analysis, and accumulation of inflammatory factors by quantitative reverse transcription polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), and western blot analysis. Then, effects of lncRNA CHRF on IL-6-treated cells were evaluated. We further explored the downstream factor of lncRNA CHRF and demonstrated whether lncRNA CHRF functioned through the downstream factor. Afterwards, crucial signaling cascades were anatomized. We found that IL-6 reduced cell viability, elevated apoptosis, induced upregulation of inflammatory factors, as well as upregulated lncRNA CHRF and down-regulated miR-146a expression. Then, we found lncRNA CHRF overexpression aggravated IL-6induced alterations, and lncRNA CHRF knockdown showed the opposite effects.Furthermore, miR-146a was identified to be negatively regulated by lncRNA CHRF, and its overexpression abrogated the roles of lncRNA CHRF in IL-6-treated cells. IL-6induced the accumulation of IκBα, p65, JAK1, and STAT3 at phosphorylated level was further facilitated by lncRNA CHRF whereas repressed by miR-146a. In conclusion, lncRNA CHRF aggravated the IL-6-induced inflammatory damages in ATDC5 cells.We further outlined a possible mechanism that through downregulating miR-146a, lncRNA CHRF evoked the activation of NF-κB and JAK1/STAT3 signaling cascades.

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Section: Introductionmentioning

confidence: 99%

Retracted: Long noncoding RNA CHRF exacerbates IL‐6‐induced inflammatory damages by downregulating microRNA‐146a in ATDC5 cells

Shi

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

S256. Long-Noncoding Rna Gas5 is Associated With Cocaine Action

Cited by 1 publication

References 0 publications

Retracted: Long noncoding RNA CHRF exacerbates IL‐6‐induced inflammatory damages by downregulating microRNA‐146a in ATDC5 cells

Retracted: Long noncoding RNA CHRF exacerbates IL‐6‐induced inflammatory damages by downregulating microRNA‐146a in ATDC5 cells

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