S53P4 bioactive glass scaffolds induce BMP expression and integrative bone formation in a critical-sized diaphysis defect treated with a single-staged induced membrane technique

Eriksson, Elin; Björkenheim, Robert; Strömberg, Gustav; Ainola, Mari; Uppstu, Peter; Aalto-Setälä, Laura; Leino, Vesa-Matti; Hupa, Leena; Pajarinen, Jukka; Lindfors, Nina

doi:10.1016/j.actbio.2021.03.035

Cited by 29 publications

(21 citation statements)

References 51 publications

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“…Still, the changes in the scaffold surface morphology depend on the ion release from the scaffold itself and are assumed to correlate with the ion release trends before a CaP layer forms on the surface, motivating the use of Tris solution alongside SBF. The long-term dissolution in SBF showed similar trends to in vivo studies [60].…”

Section: Discussionsupporting

confidence: 66%

“…In general, the overall porosity and pore size affect vascularized bone tissue formation throughout the scaffold, along with the scaffold in vivo degradation. Although the overall porosity of the scaffolds in this work was less than, e.g., in robocasted and template sintered bioactive glass scaffolds [7], similar S53P4 scaffolds showed stable scaffold integration after 8 weeks in rabbit femurs [60].…”

Section: Discussionmentioning

confidence: 99%

“…The strength of the scaffolds sintered at 630 • C for 60 min was within the strength of trabecular bone [3] also after a short SBF immersion. In an in vivo study, these scaffolds were implanted in segmental defects in rabbit femurs for 2-8 weeks and showed good bioactivity and strength: the glass degraded and CaP and new bone layers grew along the scaffold surfaces, providing additional strength [60].…”

Section: Discussionmentioning

confidence: 99%

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Dissolution of Amorphous S53P4 Glass Scaffolds in Dynamic In Vitro Conditions

et al. 2021

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The silicate-based bioactive glass S53P4 is clinically used in bone regenerative applications in granule form. However, utilization of the glass in scaffold form has been limited by the high tendency of the glass to crystallize during sintering. Here, careful optimization of sintering parameters enabled the manufacture of porous amorphous S53P4 scaffolds with a strength high enough for surgical procedures in bone applications (5 MPa). Sintering was conducted in a laboratory furnace for times ranging from 25 to 300 min at 630 °C, i.e., narrowly below the commencement of the crystallization. The phase composition of the scaffolds was verified with XRD, and the ion release was tested in vitro and compared with granules in continuous flow of Tris buffer and simulated body fluid (SBF). The amorphous, porous S53P4 scaffolds present the possibility of using the glass composition in a wider range of applications.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Dissolution of Amorphous S53P4 Glass Scaffolds in Dynamic In Vitro Conditions

et al. 2021

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“…Interestingly µCT scans showed positive effects of S53P4 on the surrounding bone tissue with increased bone volume, indicating an osteoinductive effect of the bone graft substitute, at least under non-infected conditions as seen by other groups [47,48]. This early osteoinductive effect of S53P4 could even be detected in infected conditions 4 weeks after the second surgery.…”

Section: Discussionmentioning

confidence: 68%

Treatment of Infection-Related Non-Unions with Bioactive Glass—A Promising Approach or Just Another Method of Dead Space Management?

et al. 2022

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The treatment of infected and non-infected non-unions remains a major challenge in trauma surgery. Due to the limited availability of autologous bone grafts and the need for local anti-infective treatment, bone substitutes have been the focus of tissue engineering for years. In this context, bioactive glasses are promising, especially regarding their anti-infective potential, which could reduce the need for local and systemic treatment with conventional antibiotics. The aim of this study was to investigate the osteoinductive and osteoconductive effects, as well as the anti-infectious potential, of S53P4 using a standardized non-union model, which had not been investigated previously. Using an already established sequential animal model in infected and non-infected rat femora, we were able to investigate bioactive glass S53P4 under realistic non-union conditions regarding its osteoinductive, osteoconductive and anti-infective potential with the use of µCT scans, biomechanical testing and histological, as well as microbiological, analysis. Although S53P4 did not lead to a stable union in the non-infected or the infected setting, µCT analysis revealed an osteoinductive effect of S53P4 under non-infected conditions, which was diminished under infected conditions. The osteoconductive effect of S53P4 remained almost negligible in histological analysis, even 8 weeks after treatment. Additionally, the expected anti-infective effect could not be demonstrated. Our data suggested that S53P4 should not be used in infected non-unions, especially in those with large bone defects.

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“…Therefore, the development of a biomaterial system without growth factors, but still has osteoconductive and osteoinductive properties, and can mimic the structure and function of natural bone matrix, has become the research topic. [63] Recently, Zheng et al prepared a kind of nanofibrous scaffold composed of gelatin and BG 45S5 (GF/45S5 BG), which can promote the osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro compared with neat gelatin, due to the enhanced mechanical properties as well as the ionic dissolution products after the incorporation of BG 45S5, which acts as signals to cells. For example, calcium ions can promote the proliferation and differentiation of osteoblasts, up-regulate the expression of insulin-like growth factor II (IGF-II), the production of glutamate, and the mineralization of extracellular matrix.…”

Section: Bg Scaffoldsmentioning

confidence: 99%

Research Progress of Bioactive Glass and Its Application in Orthopedics

Huang

Yu²,

et al. 2021

Adv Materials Inter

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effectively promote the expression of vascular endothelial growth factor (VEGF) in fibroblasts, and promote angiogenesis in vivo and in vitro [4] ; iii) ions and degradation products released from BGs can activate and up-regulate gene expression in bone progenitor cells, resulting in rapid bone regeneration and a higher rate of bone formation compared with other inorganic ceramics such as hydroxyapatite (HA), ttitanium dioxide, aluminium oxide. [5] In this paper, we review a series of breakthroughs and progresses in the application of BG, and discuss the future prospects of BG in bone regeneration, with the hope to provide a reference for further research on BGs.

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S53P4 bioactive glass scaffolds induce BMP expression and integrative bone formation in a critical-sized diaphysis defect treated with a single-staged induced membrane technique

Cited by 29 publications

References 51 publications

Dissolution of Amorphous S53P4 Glass Scaffolds in Dynamic In Vitro Conditions

Dissolution of Amorphous S53P4 Glass Scaffolds in Dynamic In Vitro Conditions

Treatment of Infection-Related Non-Unions with Bioactive Glass—A Promising Approach or Just Another Method of Dead Space Management?

Research Progress of Bioactive Glass and Its Application in Orthopedics

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