SA/G hydrogel containing hCAP-18/LL-37-engineered WJ-MSCs-derived conditioned medium promoted wound healing in rat model of excision injury

Sabzevari, Reza; Roushandeh, Amaneh Mohammadi; Mehdipour, Ahmad; Alini, Mauro; Roudkenar, Mehryar Habibi

doi:10.1016/j.lfs.2020.118381

Cited by 25 publications

(10 citation statements)

References 58 publications

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“…HCAP-18/LL-37 is an anti-microbial peptide secreted by phagocytes and epithelial cells with multiple functions. In addition to direct killing of pathogens, it regulates inflammatory responses and promotes wound healing by increasing the proliferation and migration of keratinocytes, as well as by stimulating neo-angiogenesis (Yang et al 2020 ; Sabzevari et al 2020 ). In pancreatic cancer, hCAP-18/LL-37 was strongly expressed by macrophages in response to tumor-derived Activin A, and increased CSC self-renewal, invasion, tumorigenicity, the expression of CD133 and of pluripotency-associated genes: KLF4, SOX2, OCT3/4 and NANOG.…”

Section: Main Textmentioning

confidence: 99%

Macrophages and cancer stem cells: a malevolent alliance

Allavena

Digifico

Belgiovine

2021

Mol Med

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Myeloid cells infiltrating tumors are gaining ever growing attention in the last years because their pro-tumor and immunosuppressive functions are relevant for disease progression and therapeutic responses. The functional ambiguity of tumor-associated macrophages (TAMs), mostly promoting tumor evolution, is a challenging hurdle. This is even more evident in the case of cancer stem cells (CSCs); as active participants in the specialized environment of the cancer stem cell niche, TAMs initiate a reciprocal conversation with CSCs. TAMs contribute to protect CSCs from the hostile environment (exogenous insults, toxic compounds, attacks from the immune cells), and produce several biologically active mediators that modulate crucial developmental pathways that sustain cancer cell stemness. In this review, we have focused our attention on the interaction between TAMs and CSCs; we describe how TAMs impact on CSC biology and, in turn, how CSCs exploit the tissue trophic activity of macrophages to survive and progress. Since CSCs are responsible for therapy resistance and tumor recurrence, they are important therapeutic targets. In view of the recent success in oncology obtained by stimulating the immune system, we discuss some macrophage-targeted therapeutic strategies that may also affect the CSCs and interrupt their malevolent alliance.

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Section: Main Textmentioning

confidence: 99%

Macrophages and cancer stem cells: a malevolent alliance

Allavena

Digifico

Belgiovine

2021

Mol Med

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“…Thirty-one studies evaluated the potential of MSC-CM for treating non-diabetic wounds (Table 1). To obtain CM, cells were mainly isolated from human tissues (74.19%, 23/31): from adipose tissue (Cho et al, 2010;Heo et al, 2011;Deng et al, 2017;Yuan et al, 2018;Chen et al, 2021) (21.74%, 5/23), amnion (Yoon et al, 2010;Jun et al, 2014;Park et al, 2018;He et al, 2020) (17.34%, 4/23), umbilical cord blood (Lee et al, 2011;Dong et al, 2017;Raj et al, 2019;Rong et al, 2019) (17.34%, 4/23), bone marrow (Tamari et al, 2013;Chen et al, 2014;Ahangar et al, 2020) (13.04%, 3/23), and Wharton's jelly (Fong et al, 2014;Tam et al, 2014;Sabzevari et al, 2020) (13.04%, 3/23). Human dental pulp (Zhou et al, 2020) and skin (Robert et al, 2019) were also used.…”

Section: Non-diabetic Woundsmentioning

confidence: 99%

Mesenchymal Stromal Cell-Conditioned Medium for Skin Diseases: A Systematic Review

Montero‐Vílchez

Sierra-Sánchez

Sánchez‐Díaz

et al. 2021

Front. Cell Dev. Biol.

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The skin is the largest organ of the human body, and its dysfunction is related to many diseases. There is a need to find new potential effective therapies for some skin conditions such as inflammatory diseases, wound healing, or hair restoration. Mesenchymal stromal cell (MSC)-conditioned medium (CM) provides a potential opportunity in the treatment of skin disease. Thus, the objective of this review is to evaluate the uses of MSC-CM for treating skin diseases in both animal and human models. A systematic review was conducted regarding the use of MSC-CM for treating skin conditions. One hundred one studies were analyzed. MSC-CM was evaluated in wound healing (55), hypertrophic scars (9), flap reperfusion (4), hair restoration (15), skin rejuvenation (15), and inflammatory skin diseases (3). MSC-CM was obtained from different MSC sources, mainly adipose tissue, bone marrow, and umbilical cord blood. MSC-CM was tested intravenously, intraperitoneally, subcutaneously, intradermally or intralesionally injected or topically applied. MSC-CM was used in both animals and humans. MSC-CM improved wound healing, hair restoration, skin rejuvenation, atopic dermatitis, and psoriasis in both animals and humans. MSC-CM also decreased hypertrophic scars and flap ischemia in animal models. In conclusion, MSC-CM is a promising therapy for skin conditions. Further studies are needed to corroborate safety and effectiveness and to standardize CM manufacturing.

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“…CM was biochemically analyzed in just one of the studies (Sabzevari et al, 2020), however, we further defined CM as "in vitro characterized" also when the corresponding cells were identified and characterized at the molecular and morphological levels. Perinatal tissues were in vitro characterized with a proportion of 50%; this group also includes commercially available perinatal tissues (71.4%).…”

Section: In Vitro Characterization and Functional Testing Of Pnd Before Their Application In Preclinical Studiesmentioning

confidence: 99%

“…For longer-term experiments on full-thickness wounds, anti-contractive tools should be chosen: Splinting wound models use silicone or rigid plastic rings strapped around the wound area to the underlying muscles to prevent wound contraction. In the reviewed papers, the silicone rings were mostly sutured or fixed with glue (Kim et al, 2012;Shohara et al, 2012;Aguilera et al, 2014;Jin et al, 2016;Wang et al, 2016;Peña-Villalobos et al, 2018;Wang et al, 2018;Kaushik and Das, 2019;Arasteh et al, 2020;Nasiry et al, 2020;Sabzevari et al, 2020). Others directly sutured scaffolds loaded with PnD to the wound site (Yang et al, 2013;Edwards et al, 2014;Milan et al, 2016;Montanucci et al, 2017).…”

Section: Wound Healing In Rodentsmentioning

confidence: 99%

Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing

Pichlsberger

Jerman

Obradović

et al. 2021

Front. Bioeng. Biotechnol.

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Knowledge of the beneficial effects of perinatal derivatives (PnD) in wound healing goes back to the early 1900s when the human fetal amniotic membrane served as a biological dressing to treat burns and skin ulcerations. Since the twenty-first century, isolated cells from perinatal tissues and their secretomes have gained increasing scientific interest, as they can be obtained non-invasively, have anti-inflammatory, anti-cancer, and anti-fibrotic characteristics, and are immunologically tolerated in vivo. Many studies that apply PnD in pre-clinical cutaneous wound healing models show large variations in the choice of the animal species (e.g., large animals, rodents), the choice of diabetic or non-diabetic animals, the type of injury (full-thickness wounds, burns, radiation-induced wounds, skin flaps), the source and type of PnD (placenta, umbilical cord, fetal membranes, cells, secretomes, tissue extracts), the method of administration (topical application, intradermal/subcutaneous injection, intravenous or intraperitoneal injection, subcutaneous implantation), and the type of delivery systems (e.g., hydrogels, synthetic or natural biomaterials as carriers for transplanted cells, extracts or secretomes). This review provides a comprehensive and integrative overview of the application of PnD in wound healing to assess its efficacy in preclinical animal models. We highlight the advantages and limitations of the most commonly used animal models and evaluate the impact of the type of PnD, the route of administration, and the dose of cells/secretome application in correlation with the wound healing outcome. This review is a collaborative effort from the COST SPRINT Action (CA17116), which broadly aims at approaching consensus for different aspects of PnD research, such as providing inputs for future standards for the preclinical application of PnD in wound healing.

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SA/G hydrogel containing hCAP-18/LL-37-engineered WJ-MSCs-derived conditioned medium promoted wound healing in rat model of excision injury

Cited by 25 publications

References 58 publications

Macrophages and cancer stem cells: a malevolent alliance

Macrophages and cancer stem cells: a malevolent alliance

Mesenchymal Stromal Cell-Conditioned Medium for Skin Diseases: A Systematic Review

Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing

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