Sa1967 The Effect of Flavonoids Luteolin and Quercetin Upon Colon Cancer Cells In Vitro; “So What's in Your Fiber?”

Chulenbayeva, Laura E.; Shaiken, Tattym E.; Opekun, Antone R.

doi:10.1016/s0016-5085(15)31240-3

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“…Studies have demonstrated that luteolin can provide resistance against oncogenic stimulation in vivo and in vitro, inhibit cell proliferation, and induce cell cycle arrest and apoptosis by stimulating or inhibiting intracellular and extracellular signaling pathways (12,13). Furthermore, the efficacy of luteolin in treating colon cancer has been previously reported (14,15). Luteolin has been indicated to induce apoptosis in colon cancer cells by arresting the cell cycle at the G2/M phase (11,13).…”

Section: Introductionmentioning

confidence: 97%

Luteolin induces mitochondrial apoptosis in HT29 cells by inhibiting the Nrf2/ARE signaling pathway

et al. 2020

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The aim of the current study was to investigate luteolin-induced apoptosis and the molecular mechanisms underlying it in HT29 cells. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to assess the cytotoxicity of luteolin on HT29 cells, and a dichloro-dihydro-fluorescein diacetate assay was used to measure cellular levels of reactive oxygen species (ROS). The effects of luteolin on the mitochondrial membrane potential were also evaluated. Bax and Bcl-2 mRNA expression were determined using reverse transcription-quantitative PCR. Additionally, western blot analysis was performed to assess changes in cytochrome c and caspase-3 protein expression. Localization of nuclear factor erythroid 2-related factor 2 (Nrf2) in the nucleus was also assessed using immunofluorescence. Luteolin exhibited cytotoxicity on HT29 cells in a time-and concentration-dependent manner. Additionally, ROS production was indicated to be increased and ROS scavenging was decreased, which resulted in a significant increase in the levels of ROS in the cells. The mitochondrial membrane potential was indicated to decrease following luteolin treatment. At the molecular level, luteolin significantly increased the mRNA expression of Bax and the protein expression of cytochrome c, caspase-3, p47 phox and p22 phox . The results revealed that luteolin decreased Bcl-2 protein expression and inhibited the nuclear localization of Nrf2. In conclusion, the current study indicated that luteolin inhibited HT29 cell proliferation and induced apoptosis via the mitochondrial pathway.

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