Sa2071 Galectin-9 Induced by Dietary Synbiotics is Involved in Suppression of Allergic Symptoms in Mice and Humans

Kivit, Sander de; Saeland, Eiríkur; Kraneveld, Aletta D.; Kant, Henk van de; Schouten, Barbara C.; Esch, Betty C.A.M. van; Knol, Jan; Sprikkelman, A. B.; Aa, L. B. van der; Knippels, L.M.J.; Kooyk, Yvette van; Garssen, Johan; Willemsen, Linette E. M.

doi:10.1016/s0016-5085(12)61496-6

Cited by 10 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The physiological relevance of in vitro studies with PBMCs is debatable, but several reports have shown that these studies can be predictive for the in vivo situation. de Kivit et al ( 2012 ) reported that increased galectin-9 expression in intestinal epithelial cells and serum induced by dietary symbiotics in mice correlated with reduced acute allergic skin reactions and mast cell degranulation. Parallel studies with stimulation of PBMCs with recombinant galectin-9, showed induction of development of Th1 and Treg cells.…”

Section: Experimental Read Outmentioning

confidence: 99%

Peripheral Blood Mononuclear Cells

Kleiveland

2015

The Impact of Food Bioactives on Health

212

154

View full text Add to dashboard Cite

Numerous cell types are involved in maintenance of the intestinal tissue. However, the main players are cells of the epithelial lining and the immune system. Human peripheral blood mononuclear cells (PBMCs) are used to investigate the effect of food bioactives on various immune cells. These cells are easily isolated from blood of healthy donors or buffy coats (leukocyte concentrates, a by-product from hospital Blood Banks in the manufacturing of red blood cell and thrombocyte concentrates from anti-coagulated whole blood). PBMCs have a different composition, phenotype and activation status than cells found in intestinal tissue. However, this is a useful test system for investigation of immune modulatory effects of food bioactive compounds. Methods for the isolation of PBMCs and how they are used to investigate effects of bioactive components are discussed in this chapter.

show abstract

Section: Experimental Read Outmentioning

confidence: 99%

Peripheral Blood Mononuclear Cells

Kleiveland

2015

The Impact of Food Bioactives on Health

212

154

View full text Add to dashboard Cite

show abstract

“…In this context, we assume that Gal-9 might be a potent regulator for (auto/ allo)-immune diseases but the murine system might only deliver limited relevance to what happens in human. Interestingly, Gal-9 has been demonstrated to support the suppression of allergic symptoms [58,59] in vivo. At the time of this study, there are 15 active clinical trials running for the MSC-based treatment of autoimmune diseases such as type 1 diabetes, multiple sclerosis, or lupus nephritis, listed at www.clinicaltrials.gov.…”

mentioning

confidence: 99%

Galectin-9 Is a Suppressor of T and B Cells and Predicts the Immune Modulatory Potential of Mesenchymal Stromal Cell Preparations

Ungerer

Quade-Lyssy

Radeke

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

Therapeutic approaches using multipotent mesenchymal stromal cells (MSCs) are advancing in regenerative medicine, transplantation, and autoimmune diseases. The mechanisms behind MSC immune modulation are still poorly understood and the prediction of the immune modulatory potential of single MSC preparations remains a major challenge for possible clinical applications. Here, we highlight galectin-9 (Gal-9) as a novel, important immune modulator expressed by MSCs, which is strongly upregulated upon activation of the cells by interferong (IFN-g). Further, we demonstrate that Gal-9 is a major mediator of the anti-proliferative and functional effects of MSCs not only on T cells but also on B cells. Here, Gal-9 and activated MSCs contribute to the suppression of antigen triggered immunoglobulin release. Moreover, we determined that Gal-9 expression could serve as a marker to predict a higher or lower immune modulatory potential of single cell preparations and therefore to distinguish the therapeutic potency of MSCs derived from different donors. Also in vivo co-administration of MSCs or murine Gal-9 resulted in significantly reduced IgG titers in mice immunized with human coagulation factor VIII (FVIII). In conclusion, Gal-9 acts as an immune modulator interfering with multiple cell types including B cells and Gal-9 may serve as a predictive indicator for clinical MSC therapy.

show abstract

“…This increased level was consistent with lowered acute allergic skin reaction and mast cell degranulation. Additionally, this pre-and pro-biotic mixture was demonstrated to augment Th1-and Treg-cell differentiation in lymph nodes and in peripheral blood mononuclear cell cultures exposed to galectin-9 [20].…”

Section: Galectin-9mentioning

confidence: 94%

Prebiotics and Probiotics in Allergy: Potential Mechanisms of Prebiotics’ and Probiotics’ Actions in Allergy - (Part 1)

Özdemir¹

2016

MOJI

View full text Add to dashboard Cite

The prevalence of allergic diseases is increasing from the last half of XXth century. This increase has been thought to be due to different factors e.g. diet as well as decreased microbial exposure (hygiene hypothesis). Gastrointestinal microbiota contributes to microbial contact during infancy and is one of the most important stimulatory factors for immature Th2-dominant neonatal immune system. Since tendency to allergy development begin to manifest early in life, there has been apparent curiosity for the possible profits of adjusting the intestinal microbiota by using pre-and/or pro-biotics. Alteration of the intestinal micro flora by giving particular non-digestible carbohydrates/fibers has received a lot of interest since the introduction of the Prebiotics concept by Gibson in 1995 and Probiotics concept by the works of Metchnikoff and Tissier.Probiotic is an oral supplement or a food product that contains a sufficient number of viable microorganisms to adapt the microbiota of the individual and has the potential for beneficial health effects. Even though the favorable effects of pre-and pro-biotics on various atopic diseases have been considered for a long time, little is identified about how pre-and pro-biotics modify the immune system and atopic disease development. In this first part of the article, our objective is to explain the possible mechanisms of pre-and pro-biotics' effects in the prevention and therapy of different allergic disease. The author thinks that better comprehension of the actions of various probiotic species on innate and adaptive immune system and further research in near future to understand into the etio-pathogenesis of the diverse manifestations of allergy are necessary for the confirmation of particular species having anti-allergic potential.

show abstract

Sa2071 Galectin-9 Induced by Dietary Synbiotics is Involved in Suppression of Allergic Symptoms in Mice and Humans

Cited by 10 publications

References 0 publications

Peripheral Blood Mononuclear Cells

Peripheral Blood Mononuclear Cells

Galectin-9 Is a Suppressor of T and B Cells and Predicts the Immune Modulatory Potential of Mesenchymal Stromal Cell Preparations

Prebiotics and Probiotics in Allergy: Potential Mechanisms of Prebiotics’ and Probiotics’ Actions in Allergy - (Part 1)

Contact Info

Product

Resources

About