Saccharification of banana agro-waste by cellulolytic enzymes

Abstract

Banana is major cash crop of this region generating vast agricultural waste after harvest. The agrowaste including dried leaves and psuedostem after harvest was used as substrate for the release of sugars. Saccharification of banana agro waste by cellulases of Trichoderma lignorum was investigated. The steam treated agro-waste yielded 1.34 mg/ml of reducing sugars after 24 hr. The size of substrate affected saccharification where the smaller size (<120 µ) yield more sugars. Maximum sugars were released at pH 6.0 whereas 40°C was the optimum temperature. Thus, under these conditions the agro waste left behind for natural degradation can be utilized affectively to yield fermentable sugars which can be converted into other substances like alcohol.

Purification and Characterization of Xylanase from Spent Mushroom Compost and its Application in Saccharification of Biomass Wastes

“…The saccharification percentage was evaluated by Eq. 1 (Baig et al 2004), Saccharification (%) = Xylose (mg/mL) / substrate (mg/mL) × 100…”

Highly Efficient Conversion Biomass of Saccharum munja for Cellulases and Xylanase Production to Ethanol Repression by Newly Isolated Trichoderma atroviride AD-130

“…The maximum production of FPase 1.01 U mL -1 , CMCase (2.69 U mL -1 ), b-glucosidase (0.82 U mL -1 ) and xylanase (82.99 U mL -1 ) by Trichoderma atrovirideAD-130 was observed at 30°C. Further increase in incubation temperature from 30 to 33°C or above resulted in decreased cellulase and xylanase production.Contrary to the above results, different cellulolytic fungi were found to produce maximum cellulases at 28°C, for example, T. Viride (Zhou et al, 2008), T. Harzianum (Ahmad Idi and Mohamad S. Eva (2011), T. Asperullum (Chen Y et al, 2010) and A. niger (Harun M.Y et al,2011) yielded maximum cellulases at 28°C.On the other hand, Baig et al (2004) reported maximum cellulases production by T. Reesei at 30°C while (Ahamed, A., and Vermette, P 2008) found maximum cellulases and xylanase activity by Aspergillus fumigates at 30°C. However, as many researchers had reported on variety of optimum temperatures, it could be suggested that the optimal condition for cellulases production depended not only upon the variation of the microorganism (Sukhang et al, 2020) but also the temperature variation of the culture conditions.…”

Statistical optimization of nutritional and physical parameters for xylanase production from newly isolated Aspergillus oryzae LC1 and its application in the hydrolysis of lignocellulosic agro-residues

“…Every 2 h, the reaction mixture was withdrawn, centrifuged at RCF of 2350 g at 4 °C for 15 min, and the supernatants were used for the quantification of the reducing sugar content by the 3,5-dinitrosalicylic acid method using xylose as a standard. The saccharification percentage was calculated by the equation given by Baig et al (2004), which is shown below (Eq. 2):…”