Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by <i> Aspergillus fumigatus</i>

Lamounier, Kênia Francisca Resende; Rodrigues, Patrisia O.; Pasquini, Daniel; Baffi, Milla Alves

doi:10.7569/jrm.2017.634151

Cited by 12 publications

(18 citation statements)

References 10 publications

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“…Hence, degradation of lignin may assist the action of cellulases and hemicellulases enzymes on cellulose and hemicellulose, respectively. Hydrolysis of hemicellulose and cellulose in alkaline pretreatment is less when compared with acid treated samples [28,26]. From the above results it clear that, observed data are in agreement with text which reports that alkaline pretreatment preferentially removes lignin [29,26], and acid pretreatment degrades hemicellulose fraction [30,22].…”

Section: Discussionsupporting

confidence: 80%

“…4c). Lamounier et al, [26] also reported that maximum reducing sugar production during sacchari cation at 55 °C. Further increased temperature beyond 55 °C, the concentration of reducing sugar and sacchari cation rate were reduced.…”

Section: Discussionmentioning

confidence: 94%

“…In the chemical analysis, it was found that after alkaline pretreatment the proportion of cellulose and hemicellulose increased by 33 and 27%, respectively, while lignin decreased by 44%. Lamounier et al, [26] also reported that after alkali pretreatment, lignin content of sugarcane bagasse was decreased by 43%. These results were previously predictable, because alkali works primarily on lignin, promoting its degradation.…”

Section: Discussionmentioning

confidence: 94%

“…In contrast to our result, Moretti et al, [25] observed 46.9% cellulose, 16.3% hemicellulose, 27.1% lignin, and 2.0% ash in sugarcane bagasse. Lamounier et al, [26] observed 54.4% cellulose, 13.5% hemicellulose, 26.1% total lignin, and 0.6% ash in sugarcane bagasse.…”

Section: Discussionmentioning

confidence: 96%

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Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Tiwari¹,

Yadav²,

Gaur³

et al. 2020

Preprint

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Background: Enhancement of cellulase and xylanase production and improvement of more proficient lignocellulose-degrading enzymes are essential in order to decrease the price of enzymes required in the biomass-to-bioethanol production. Results: The effectiveness of different concentration of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of cellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% cellulose, 25.63% hemicelluloses, 30.36% lignin, 1.44% ash and 2.90% other extractives. Afterthat, The sugarcane bagasse was pretreated with two different concentrations (5% and 10%) of H2SO4 and NaOH at 121°C for 60 min. Among them, the best result was obtained when sugarcane bagasse was pretreated with 10% NaOH solution followed by 10% H2SO4, 5% NaOH and 5% H2SO4 solution. The highest cellulose saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75mg/g, 301.25 mg/g and 276.6 mg/g from 10% H2SO4, 5% NaOH and 5% H2SO4, respectively, which were 55.1, 32.0, 27.1 and 20.6 times higher than the control. Moreover, the FTIR, XRD and SEM analysis showed significant molecule and surface structure changes of the sugarcane bagasse after different pretreatments. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] was used to hydrolyze the pretreated sugarcane bagasse and the optimal condition was determined to be 30 h of enzymatic reaction with 3:1 ration of enzymes under the temperature of 55°C, pH 5.5, substrate concentration of 3% and Tween-20 0.5%. Conclusion: Enzyme supernatants produced by the mixed culture of Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] on various pretreated sugarcane baggase have good cellulase and xylanase activities, leading to celluloses and Hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 96%

See 2 more Smart Citations

Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Tiwari¹,

Yadav²,

Gaur³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In contrast to our result, Moretti et al, [36] The compositional analysis of the un-treated and the pretreated sugarcane bagasse samples showed that after alkaline pretreatment the proportion of cellulose and hemicellulose increased by 33 and 27%, respectively, while lignin decreased by 44%. Lamounier et al, [37] also reported that after alkali pretreatment, lignin content of sugarcane bagasse was decreased by 43%. These results were previously predictable, because alkali works primarily on lignin, promoting its degradation.…”

Section: Chemical Analysismentioning

confidence: 94%

Assessment of a novel pretreatment techniques for enhancing the enzymatic saccharification of sugarcane baggase: Structural and chemical analysis

Tiwari¹,

Yadav²,

Gaur³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Enhancement of cellulase and xylanase production and improvement of more proficient lignocellulose-degrading enzymes are essential in order to decrease the price of enzymes required in the biomass-to-bioethanol production. Results The effectiveness of different concentration of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of cellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% cellulose, 25.63% hemicelluloses, 30.36% lignin, 1.44% ash and 2.90% other extractives. Afterthat, The sugarcane bagasse was pretreated with two different concentrations (5% and 10%) of H 2 SO 4 and NaOH at 121°C for 60 min. Among them, the best result was obtained when sugarcane bagasse was pretreated with 10% NaOH solution followed by 10% H 2 SO 4 , 5% NaOH and 5% H 2 SO 4 solution. The highest cellulose saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75mg/g, 301.25 mg/g and 276.6 mg/g from 10% H 2 SO 4 , 5% NaOH and 5% H 2 SO 4 , respectively, which were 55.1, 32.0, 27.1 and 20.6 times higher than the control. Moreover, the FTIR, XRD and SEM analysis showed significant molecule and surface structure changes of the sugarcane bagasse after different pretreatments. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] was used to hydrolyze the pretreated sugarcane bagasse and the optimal condition was determined to be 30 h of enzymatic reaction with 3:1 ration of enzymes under the temperature of 55°C, pH 5.5, substrate concentration of 3% and Tween-20 0.5%. Conclusion Enzyme supernatants produced by the mixed culture of Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] on various pretreated sugarcane baggase have good cellulase and xylanase activities, leading to celluloses and Hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

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Ethanol Production and Other Bioproducts by Galactomyces geotrichum from Sugarcane Bagasse Hydrolysate

et al. 2020

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Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by Aspergillus fumigatus

Cited by 12 publications

References 10 publications

Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

Assessment of a novel pretreatment techniques for enhancing the enzymatic saccharification of sugarcane baggase: Structural and chemical analysis

Ethanol Production and Other Bioproducts by Galactomyces geotrichum from Sugarcane Bagasse Hydrolysate

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