Saccharomyces cerevisiae: a versatile eukaryotic system in virology

Galão, Rui Pedro; Scheller, Nicoletta; Alves-Rodrigues, Isabel; Breinig, Tanja; Meyerhans, Andreas; Díez, Juana

doi:10.1186/1475-2859-6-32

Cited by 43 publications

(35 citation statements)

References 88 publications

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“…Due to its cutting-edge role, it is not surprising that the yeast S. cerevisiae has become a wellestablished eukaryotic model organism to study fundamental biological processes such as aging (29), mRNA transport (231), the cell cycle (35), and many more. S. cerevisiae also serves as a model organism for studying human diseases such as cancer (326,396) and has been used as a tool for drug research (222), studying prions (55), basic and applied virus research (91), and ecotoxicology (346).…”

Section: Introductionmentioning

confidence: 99%

Progress in Metabolic Engineering of Saccharomyces cerevisiae

Nevoigt

2008

Microbiol Mol Biol Rev

528

333

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SUMMARY The traditional use of the yeast Saccharomyces cerevisiae in alcoholic fermentation has, over time, resulted in substantial accumulated knowledge concerning genetics, physiology, and biochemistry as well as genetic engineering and fermentation technologies. S. cerevisiae has become a platform organism for developing metabolic engineering strategies, methods, and tools. The current review discusses the relevance of several engineering strategies, such as rational and inverse metabolic engineering, evolutionary engineering, and global transcription machinery engineering, in yeast strain improvement. It also summarizes existing tools for fine-tuning and regulating enzyme activities and thus metabolic pathways. Recent examples of yeast metabolic engineering for food, beverage, and industrial biotechnology (bioethanol and bulk and fine chemicals) follow. S. cerevisiae currently enjoys increasing popularity as a production organism in industrial (“white”) biotechnology due to its inherent tolerance of low pH values and high ethanol and inhibitor concentrations and its ability to grow anaerobically. Attention is paid to utilizing lignocellulosic biomass as a potential substrate.

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Section: Introductionmentioning

confidence: 99%

Progress in Metabolic Engineering of Saccharomyces cerevisiae

Nevoigt

2008

Microbiol Mol Biol Rev

528

333

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“…The replication of the plant BMV in the yeast Saccharomyces cerevisiae is a fruitful model system for studying common and fundamental steps of (+)RNA virus biology in a relatively simple background Galao et al 2007). The BMV genome consists of three RNAs (RNA1, RNA2, and RNA3) that are 5 ′ capped (Fig.…”

Section: Introductionmentioning

confidence: 99%

The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms

Jungfleisch

Chowdhury

Alves-Rodrigues

et al. 2015

RNA

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The Lsm1-7-Pat1 complex binds to the 3 ′ end of cellular mRNAs and promotes 3 ′ end protection and 5 ′ -3 ′ decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes.

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“…Although plants and yeast are widely used as vehicles for oral vaccine delivery (Mason et al, 2002;Streatfield, 2006;Galao et al, 2007), high-level expression of the heterologous antigen protein is difficult to achieve in practice (Rice et al, 2005). Because there is an intrinsic limit to the level of antigen expression in transgenic organisms, the antigen may be fused to cholera toxin or to the heat-labile E. coli enterotoxin, both of which are capable of targeting antigen to oral mucosal site to obtain an efficient delivery of the limited number of antigen proteins to mucosal immune induction site (Liljeqvist et al, 1997;Harakuni et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Antigenic Determinants in ApxIIA Exotoxin Capable of Inducing Protective Immunity toActinobacillus pleuropneumoniaeChallenge

Seo

Kim

et al. 2011

Immunological Investigations

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Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia. Among the virulence factors of the pathogen, ApxIIA, a bacterial exotoxin, is expressed by many serotypes and presents a plausible target for vaccine development. We characterized the region within ApxIIA that induces a protective immune response against bacterial infection using mouse challenge model. Recombinant proteins spanning the length of ApxIIA were produced and antiserum to the full-length ApxIIA was induced in mice. This antiserum recognized fragments #2, #3 and #5 with high binding specificity, but showed poor recognition for fragments #1 and #4. Of the antisera induced in mice by injection of each fragments, only the antiserum to fragment #4 failed to efficiently recognize the full-length antigen, although the individual antisera recognized their cognate antigens with almost equal efficiency. The protective potency of the immunogenic proteins against a challenge injection of bacteria in vivo correlated well with the antibody titer. Fragment #5 induced the highest level of protective activity, comparable to that by the full-length protein. These results support the use of fragment #5 to produce a vaccine against A. pleuropneumoniae challenge, since the small antigen peptide is easier to handle than is the full-length protein and can be expressed efficiently in heterologous expression systems.

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Saccharomyces cerevisiae: a versatile eukaryotic system in virology

Cited by 43 publications

References 88 publications

Progress in Metabolic Engineering of Saccharomyces cerevisiae

Progress in Metabolic Engineering of Saccharomyces cerevisiae

The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms

Characterization of Antigenic Determinants in ApxIIA Exotoxin Capable of Inducing Protective Immunity toActinobacillus pleuropneumoniaeChallenge

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