2021
DOI: 10.3390/bioengineering8030036
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Saccharomyces cerevisiae Concentrates Subtoxic Copper onto Cell Wall from Solid Media Containing Reducing Sugars as Carbon Source

Abstract: Copper is essential for life, but it can be deleterious in concentrations that surpass the physiological limits. Copper pollution is related to widespread human activities, such as viticulture and wine production. To unravel aspects of how organisms cope with copper insults, we used Saccharomyces cerevisiae as a model for adaptation to high but subtoxic concentrations of copper. We found that S. cerevisiae cells could tolerate high copper concentration by forming deposits on the cell wall and that the copper-c… Show more

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Cited by 3 publications
(4 citation statements)
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“…kluyveri class 1A DHODH (SC, TC, LK1A, respectively) were viable in medium containing 2000 μM copper and uracil, implying no toxic effects of copper accumulation. In line with previous observations, cells were slightly darker in colour due to accumulation of copper sulfide [ 82 , 83 ]. Transformants were then assessed for their ability to grow in the absence of pyrimidines in various inducing conditions, indicative of DHODH activity ( Fig 2A ).…”
Section: Resultssupporting
confidence: 92%
See 1 more Smart Citation
“…kluyveri class 1A DHODH (SC, TC, LK1A, respectively) were viable in medium containing 2000 μM copper and uracil, implying no toxic effects of copper accumulation. In line with previous observations, cells were slightly darker in colour due to accumulation of copper sulfide [ 82 , 83 ]. Transformants were then assessed for their ability to grow in the absence of pyrimidines in various inducing conditions, indicative of DHODH activity ( Fig 2A ).…”
Section: Resultssupporting
confidence: 92%
“…To ensure an overabundance of copper is not toxic to yeast cells, active class 1A DHODH were induced with a high concentration of copper in pyrimidine-rich media and viability assessed (S1A Fig) . Indeed, ura1Δ strains expressing either S. cerevisiae, T. cruzi or L. kluyveri class 1A DHODH (SC, TC, LK1A, respectively) were viable in medium containing 2000 μM copper and uracil, implying no toxic effects of copper accumulation. In line with previous observations, cells were slightly darker in colour due to accumulation of copper sulfide [82,83]. Transformants were then assessed for their ability to grow in the absence of pyrimidines in various inducing conditions, indicative of DHODH activity (Fig 2A).…”
Section: Rhizopus and Mucor Putative Class 1a Dhodh Genes Complement ...supporting
confidence: 84%
“…Because of its low cost, stable source, large quantity, easy acquisition, safety, and other characteristics, it has become an effective technique for removing copper from wine. High copper concentrations can be dealt with by yeast during fermentation (Ruta & Farcasanu, 2021). In the process of wine fermentation, the reproduction, growth, and decline of yeast cells are accompanied by the adsorption of living and dead yeast cells.…”
Section: Biosorptionmentioning
confidence: 99%
“…The copper deposits on the cell wall could be increased by disrupting the low-affinity copper intake through the plasma membrane. Based on this mechanism, we could propose a helpful approach for removing excess copper from different contaminated solids [12].…”
mentioning
confidence: 99%