Saccharomyces cerevisiae strains as bioindicators for titanium dioxide sunscreen photoprotective and photomutagenic assessment

Diniz, Raiane R.; Paiva, Juliana P.; Aquino, Renan Marcos Araújo de; Gonçalves, Tula C.W.; Leitão, Álvaro C.; Santos, Bianca Aloise Maneira Corrêa; Pinto, Alicia Viviana; Leandro, Kátia Christina; Pádula, Marcelo de

doi:10.1016/j.jphotobiol.2019.111584

Cited by 4 publications

(9 citation statements)

References 45 publications

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“…The AWP001 mutant (yno1: KanMX6) strain was used in this study (Diniz et al 2019). Yeast cultures were grown at 28°C in Yeast Peptone Dextrose (YPD) liquid medium (1% yeast extract, 1% bacto peptone, and 2% glucose-BactoTM, Merck).…”

Section: Yeast Strain Media and Growth Conditionsmentioning

confidence: 99%

“…The yeast assay was performed using the methodology de ned by Diniz et al (2019). For that, yeast cultures (10 ml) of AWP001 (yno1) S. cerevisiae strain were grown to a cell density of ∼1×10 8 cells/ml at 28 ºC with agitation for 48 h (stationary phase).…”

Section: Solar Simulated Light (Ssl)mentioning

confidence: 99%

“…The AWP001 (yno1) strain is de cient in Yno1 protein, which is a yeast NADPH oxidase protein that is ortholog to the NADPH oxidase (NOX) gene involved in UVB induced stress repair signaling (Rinnerthaler et al 2012;Glady et al 2018). In this sense, AWP001 (yno1) strain displays a more suitable yeast model for in vitro photoprotective and photo/antiphotomutagenicity assessment using irradiation protocols with UV doses and exposure times comparable to environmental sunlight (Diniz et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Photoprotective and antiphotomutagenic effect of Baccharis dracunculifolia optimized extract in the alternative Saccharomyces cerevisiae model

Vilas-Boas¹,

Diniz,

Faraco

et al. 2024

Preprint

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The recent discovery of hazardous side effects associated with sunscreen ingredients has prompted the search for natural alternatives that can effectively absorb UV rays. This study focuses on evaluating the chemical profile and potential photoprotective and antiphotomutagenic effects of an ethanolic extract from Baccharis dracunculifolia using the Saccharomyces cerevisiae model. An analysis conducted using ESI-QToF-LC-MS confirmed the presence of flavonoids and phenylpropanoids in the B. dracunculifolia extract, including compounds such as apigenin, hydroxycinnamic acid derivatives, caffeoylquinic acids, and chlorogenic acid. In the yeast model, the extract demonstrated the ability to protect S. cerevisiae yno1 strain cells against cytotoxic lesions induced by high Solar Simulated Light (SSL) irradiation exposure. Furthermore, the extract exhibited antioxidant properties, as evidenced by its protection against SSL-induced oxidative damage in the yno1 strain, which is an indicator of oxidative damage. Additionally, the extract showed potential as an antiphotomutagenic agent, as the yno1-treated strain displayed a lower frequency of photomutagenicity in terms of CanR/107 mutant cells, thereby reducing both lethal cytotoxic lesions induced by SSL and protecting the cells against SSL-induced oxidative mutagenic lesions. These findings suggest that the B. dracunculifolia extract holds promise as a natural and effective photoprotective ingredient, offering a green alternative to traditional sunscreens by reducing the need for harmful chemical filters.

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Section: Yeast Strain Media and Growth Conditionsmentioning

confidence: 99%

Section: Solar Simulated Light (Ssl)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photoprotective and antiphotomutagenic effect of Baccharis dracunculifolia optimized extract in the alternative Saccharomyces cerevisiae model

Vilas-Boas¹,

Diniz,

Faraco

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yeast cultures were grown at 28°C in Yeast Peptone Dextrose (YPD) liquid medium (1% yeast extract, 1% bactopeptone, and 2% glucose). YPD solid medium (1% yeast extract, 1% bactopeptone, 2% glucose, and 2% agar) was used for photoprotection assessment after the treatments (Diniz et al, 2019).…”

Section: Yeast Strain Media and Growth Conditionsmentioning

confidence: 99%

In vitro approaches to antioxidant screening for the development of a sunscreen formulation

Soares,

Diniz,

Paiva

et al. 2023

Braz. J. Pharm. Sci.

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The incorporation of antioxidants into sunscreens may provide additional skin photoprotection against the harmful photobiological effects of ultraviolet radiation. The present study evaluated the applicability of a screening approach to the assessment of the antioxidant and photoprotective properties of vitamin C, vitamin E, and coenzyme Q10 and then determined the performance of the most effective antioxidant in a sunscreen formulation. Antioxidant activity was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2`-azino-bis (3-ethylbenzothiazoline-6sulfonic acid) (ABTS) assay, and oxygen radical absorbance capacity (ORAC) assay, and the photoprotective potential was investigated by the yeast photoprotection assay. The antioxidant with the best effect was incorporated into sunscreen formulations which were evaluated for 120 days regarding their in vitro photoprotective parameters. Vitamin C showed high antioxidant capacity as well as a photoprotective potential against simulated solar irradiation applied for times longer than 1 h. Although the Sun Protection Factor, UVA/UVB ratio and critical wavelength did not differed significantly (p < 0.05) between the formulation blank and the formulations containing 0.5% or 1% vitamin C, formulations with vitamin C kept their photostability for 6 months. Consequently, the proposed screening approach seems to be promising for the development of an antisolar photostable formulation containing vitamin C as an antioxidant.

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“…The review of previous literature highlights that the previous studies have involved different aspects regarding the use of yeast in pretesting sunscreens [2] [25] [26]. It is also found that sunscreens are assumed to avoid exposure to ultraviolet radiation and are designed to minimize the effects of these radiations.…”

Section: Sunscreens and Yeast Coloniesmentioning

confidence: 99%

The Effect of Sunscreens on Yeast to Prevent Ultraviolet Damage

Alhamdy¹,

Al-Sowayan²

2020

ABB

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The impacts of Ultraviolet (UV) radiation on the human body have received increased attention in previous years. The impacts are comprised of a series of illnesses, burns, and modifications in the genetic structures. Therefore, the current study aimed to identify the effect of sunscreens on yeast to prevent ultraviolet damage. The study uses two sunscreens with different sun protection factors to show how they differently protect the cells from the wrath of UV light. The experiment involves the use of yeast as the test organism since yeast has DNA that is used for reproduction by budding. UV light affects the growth of the bacteria and the limiting factor with the sunscreens. Six Petri dishes with agar were inoculated with yeast solution and put under different conditions and the growth results will be obtained. The experiment also showed how different clothes protect human from the UV radiations.

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Saccharomyces cerevisiae strains as bioindicators for titanium dioxide sunscreen photoprotective and photomutagenic assessment

Cited by 4 publications

References 45 publications

Photoprotective and antiphotomutagenic effect of Baccharis dracunculifolia optimized extract in the alternative Saccharomyces cerevisiae model

Photoprotective and antiphotomutagenic effect of Baccharis dracunculifolia optimized extract in the alternative Saccharomyces cerevisiae model

In vitro approaches to antioxidant screening for the development of a sunscreen formulation

The Effect of Sunscreens on Yeast to Prevent Ultraviolet Damage

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