Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp.nov., novel actinomycetes from soil.

Lu, Zhitang; Liu, Zhiheng; Wang, Liming; Zhang, Yamei; Qi, Weihong; Goodfellow, Michael

doi:10.1099/00207713-51-2-319

Cited by 67 publications

(17 citation statements)

References 32 publications

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“…Some sequences were obtained from the public sequence databanks, but the vast majority were determined and deposited during the current study. DNA was isolated and approximately 1320 almost-complete 16S rRNA gene sequences were determined in the various collaborating laboratories using previously described methods (Chun and Goodfellow 1995;Goodfellow et al 2007;Kim et al 1996;Kumar and Goodfellow 2008;Labeda and Kroppenstedt 2000;Labeda et al 2009;Lu et al 2001;Park et al 2005).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic study of the species within the family Streptomycetaceae

Labeda

Goodfellow

Brown

et al. 2011

Antonie van Leeuwenhoek

223

186

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Species of the genus Streptomyces, which constitute the vast majority of taxa within the family Streptomycetaceae, are a predominant component of the microbial population in soils throughout the world and have been the subject of extensive isolation and screening efforts over the years because they are a major source of commercially and medically important secondary metabolites. Taxonomic characterization of Streptomyces strains has been a challenge due to the large number of described species, greater than any other microbial genus, resulting from academic and industrial activities. The methods used for characterization have evolved through several phases over the years from those based largely on morphological observations, to subsequent classifications based on numerical taxonomic analyses of standardized sets of phenotypic characters and, most recently, to the use of molecular phylogenetic analyses of gene sequences. The present phylogenetic study examines almost all described species (615 taxa) within the family Streptomycetaceae based on 16S rRNA gene sequences and illustrates the species diversity within this family, which is observed to contain 130 statistically supported clades, as well as many unsupported and single member clusters. Many of the observed clades are consistent with earlier morphological and numerical taxonomic studies, but it is apparent that insufficient variation is present in the 16S rRNA gene sequence within the species of this family to permit bootstrap-supported resolution of relationships between many of the individual clusters.

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Section: Methodsmentioning

confidence: 99%

Phylogenetic study of the species within the family Streptomycetaceae

Labeda

Goodfellow

Brown

et al. 2011

Antonie van Leeuwenhoek

223

186

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“…PCR amplification of the 16S rDNA was carried out as described by Kim et al (1996) and the resultant PCR products were purified by using the Wizard PCR purification system (Promega), following the manufacturer's instructions. The purified products were sequenced directly using a Taq Dye Deoxy Terminator cycle sequencing kit (Applied Biosystems) and two universal primers described previously (Lu et al, 2001). Sequence gel electrophoresis was carried out and the nucleotide sequence was obtained automatically by using an Applied Biosystems DNA sequencer (model 373A) and software provided by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

Nocardia beijingensis sp. nov., a novel isolate from soil.

Wang¹,

Zhang²,

Lu³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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The taxonomic status of a soil isolate, provisionally assigned to the genus Nocardia, was established following a polyphasic study. An almost complete sequence of the 16S rDNA of the strain was determined and aligned with available sequences for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organism was associated consistently with the type strains of Nocardia brasiliensis and Nocardia farcinica in the neighbour-joining analysis, albeit with low bootstrap values. It was also found to have chemotaxonomic and morphological properties consistent with its assignment to the genus Nocardia. The organism was readily separated from representatives of other Nocardia species by a broad range of phenotypic properties. The name proposed for the novel species is Nocardia beijingensis sp. nov. The type strain is AS4.1521 T (l IFO 16342 T l JCM 10666 T ).

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“…The genus Saccharopolyspora was proposed by [7] originally isolated from spontaneously heated sugarcane bagasse. Representatives of these species form a distinct phyletic line within the evolutionary radiation encompassed by the family Pseudonocardiaceae can be distinguished by using phenotypic properties [8] . Members of the genus Saccharopolyspora are characterized by aerobic, extensively branched substrate hyphae that fragment into rod-shaped elements and aerial hyphae that segment into bead-like chains of spores.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic and Antimicrobial Potential of Actinomycete Species Saccharopolyspora salina VITSDK4 Isolated from the Bay of Bengal Coast of India

Suthindhiran¹,

Каннабиран²

2009

American J. of Infectious Diseases

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Problem statement: Although the diversity of marine Actinobacteria have been studied and biotechnologically exploited throughout the world, the studies on marine actinobacteria in Indian peninsula are largely unexplored. Of 9 maritime states in India, only 4 states have been extensively studied for the diversity of actinobacteria. Further, the studies on bioactive actinomycetes from saline soil are very scanty. In the present study, we had taken an initiative to isolate culturable halophilic actinomycetes and to screen the bioactive potential. Approach: The marine sediment sample was collected at a depth of 400 cm at Marakkanam. The strain was isolated using ISP No. 2 medium supplemented with 25% sea water. The polyphasic taxonomy of the strain was evaluated by phenotypic, chemotaxonomic and phylogenetic analysis. The 16S rRNA was sequenced and phylogenetic relationship with the closest related species were studied. The growth conditions and the medium had been optimized under shake-flask conditions by measuring the dry weight of the mycelium. The isolate was subjected to fermentation and the crude extract was screened for cytotoxic, hemolytic and antimicrobial activity. The cytotoxicity was evaluated on HeLa cells by MTT assay, hemolytic assay on mouse erythrocytes and the antimicrobial activity was determined by agar diffusion assay. Results: Based on polyphasic taxonomy the species was identified as Saccharopolyspora salina and belongs to the genus Saccharopolyspora. The 16S rRNA sequence of the isolate showed 100% similarity with Saccharopolyspora salina. Based on the phylogenetic and phenotypic evaluation the isolate was designated as Saccharopolyspora salina VITSDK4. The growth was maximal in the designed production medium with the incubation temperature of 28°C and pH of 7.4. It is a moderately halophilic species requires 9% NaCl concentration for optimal growth. The cytotoxicity on HeLa cells showed the IC 50 value of 26.2 µg mL −1 by MTT assay. The hemolytic activity on mouse erythrocytes showed the EC 50 value of 266 µg mL −1 . The crude extract also exhibited significant antagonistic activity against fungal pathogens Aspergillus niger, Aspergillus fumigatus, Candida albicans and the Gram negative bacteria Escherichia coli and Klebsiella pneumoniae. Only moderate activity was seen against Gram positive bacteria Staphylococcus aureus and Bacillus subtilis. Conclusion/Recommendations: Based on the results of our investigation, the isolated strain was identified as Saccharopolyspora salina VITSDK4, which was moderately halophilic, produces an extracellular bioactive metabolite, which inhibits the proliferation of HeLa cells as well as antagonistic to fungal and bacterial pathogens. Further studies on purification and characterization of the pure compound from the strain were ongoing. The results of this study suggested that the marine actinomycetes from the unexplored Indian coast could provide lead compounds of therapeutic value.

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Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp.nov., novel actinomycetes from soil.

Cited by 67 publications

References 32 publications

Phylogenetic study of the species within the family Streptomycetaceae

Phylogenetic study of the species within the family Streptomycetaceae

Nocardia beijingensis sp. nov., a novel isolate from soil.

Cytotoxic and Antimicrobial Potential of Actinomycete Species Saccharopolyspora salina VITSDK4 Isolated from the Bay of Bengal Coast of India

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