Saccharopolyspora spinosa sp. nov. Isolated from Soil Collected in a Sugar Mill Rum Still

Mertz, Frederick P.; Yao, Raymond C.

doi:10.1099/00207713-40-1-34

Cited by 287 publications

(175 citation statements)

References 17 publications

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“…S pinosad is a macrocyclic lactone derived from the fungus Saccharopolyspora spinosa (Mertz & Yao, 1990) The two most abundant components produced from fermentation of S. spinosa are spinosyns A and D (Fig. 1), which are the major active components of the flea product spinosad (Kirst et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of speed and duration of efficacy of spinosad tablets for treatment and control ofCtenocephalides canis(Siphonaptera: Pulicidae) infestations in dogs

Franc

Bouhsira

2009

Parasite

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Summary :A controlled clinical trial was performed to determine the duration of efficacy of a new oral insecticide formulation of spinosad for the control of experimentally induced Ctenocephalides canis infestations in dogs. Twelve Beagle dogs (two groups of six) were used in the study. Dogs in the treated group received spinosad tablets per os on D0 at the commercial dosage. All dogs were infested with 100 fleas on 7, 14, 21, 28 and 35. The dogs were combed four hours after each infestation and fleas were counted and replaced on the coat. 24 hours after each infestation fleas were combed, counted and removed. The efficacy of the formulation was calculated four and 24 hours after the treatment and then four and 24 hours after each new infestation. The mean number of fleas on the control dogs was respectively between 65.1 and 83.3 at four hour counts and between 58.3 and 75.3 at 24 hour counts. The product was well tolerated. The treatment controlled the fleas already present on the skin with 81 % efficacy at four hours and 100 % efficacy at 24 hours. For the weekly infestations, the speed of action of the product was high: at four hours the efficacy was 100 % at D7, 96 % at D14, 74 % at D21, 42 % at D28, 12.90 % at D35 and 12.8 % at D42. The efficacy evaluated 24 hours after each infestation was approximately 100 % during three weeks then 90 % at D39, 81.4 % at D36 and 80.4 % at D43. A single dose of the new spinosad tablet formulation should control flea populations in dogs for four weeks as indicated in the claim (evaluation performed at 48 h for the registration). Spinosad tablet is the first product administered per os which acts so long and so quickly against adult fleas. Résumé

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Section: Introductionmentioning

confidence: 99%

Evaluation of speed and duration of efficacy of spinosad tablets for treatment and control ofCtenocephalides canis(Siphonaptera: Pulicidae) infestations in dogs

Franc

Bouhsira

2009

Parasite

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“…S. spinosa ATCC 49460 (Mertz & Yao, 1990) was the host for some transformation and bacteriophage plaque-formation studies. S. spinosa PM89 is a novobiocin-treated derivative of ATCC 49460 lacking the plasmid pSASl (P. Matsushima & R. H. Baltz, unpublished).…”

Section: Methodsmentioning

confidence: 99%

“…Saccbaropolys-ora spinom was isolated from soil in a defunct sugar-mill rum still in the Virgin Islands (Mertz & Yao, 1990). S ' .…”

Section: Introductionmentioning

confidence: 99%

Transformation of Saccharopolyspora spinosa protoplasts with plasmid DNA modified in vitro to avoid host restriction

Matsushima

Baltz

1994

Microbiology

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Indiana poi is, I n diana 46285, USA Saccharopolyspora spinosa protoplasts were not transformable by several different streptomycete plasmids, and S. spinosa was not a host for plaque formation by the Saccharopolyspora bacteriophages 4SE6,4SE45, 4SE57, 4SE60, 4SE69 or HP10. Extracts of S. spinosa contained DNA-modifying activities that blocked cleavage of plasmid DNA by Nael and Sall, and partially blocked cleavage by Ncol. Plasmid pOJ434, a derivative of the 5. spinosa plasmid pSAS1, was modified in vitro by incubation with an extract of S. spinosa to circumvent restriction. Under optimal conditions, 5. spinosa protoplasts were transformed to apramycin resistance by modified pOJ434 at frequencies of about lo4 per pg of DNA. Transformants contained pOJ434 primarily integrated in the chromosome.

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“…It can survive in the presence of 11% NaCl. 36) In our extraction experiment, the mycelium was ground into powder in liquid nitrogen to improve extraction efficiency. Previous studies have found that an accumulation of intracellular ectonie can help microbes to resist high osmotic pressure.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Metabolomics Analysis ofSaccharopolyspora spinosaWT, WH124, and LU104 Revealed Metabolic Mechanisms Correlated with Increases in Spinosad Yield

Zhao

Xue

Wang

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Saccharopolyspora spinosa sp. nov. Isolated from Soil Collected in a Sugar Mill Rum Still

Cited by 287 publications

References 17 publications

Evaluation of speed and duration of efficacy of spinosad tablets for treatment and control ofCtenocephalides canis(Siphonaptera: Pulicidae) infestations in dogs

Evaluation of speed and duration of efficacy of spinosad tablets for treatment and control ofCtenocephalides canis(Siphonaptera: Pulicidae) infestations in dogs

Transformation of Saccharopolyspora spinosa protoplasts with plasmid DNA modified in vitro to avoid host restriction

A Comparative Metabolomics Analysis ofSaccharopolyspora spinosaWT, WH124, and LU104 Revealed Metabolic Mechanisms Correlated with Increases in Spinosad Yield

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