Safety and efficacy of a novel inactivated trivalent Salmonella enterica vaccine in chickens

Crouch, Colin; Nell, T.; Reijnders, M.P.; Donkers, Ton; Pugh, Chris; Patel, Amit; Davis, P A; Hulten, M.C.W. van; Vries, Stefan P. W. de

doi:10.1016/j.vaccine.2020.08.033

Cited by 23 publications

(20 citation statements)

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“…Veterinary Medicine International [11] reported that there were no clinical signs of depression or disease in chickens vaccinated with (AviPro ® Salmonella Vac E, ELANCO) (live commercial attenuated Salmonella enteritidis vaccine based on strain Sm24/Rif12/Ssq) since vaccination of broiler breeder hens with trivalent inactivated Salmonella vaccine had provided a safe and efficacious reduction of both intestinal colonization and organ invasion with related serovars of Salmonella enterica following challenge. Subsequently, it could be used for the control programme to reduce contamination rates of poultry flocks and products [12], so inactivated Salmonella enteriditis phage types (especially 3A and 6A) could play an effective role in the control of Salmonella enteriditis infection in Malaysia. e presence of mortality in the control group CV0C and the absence of mortality in the inoculated and challenged groups gave evidence that inactivated Salmonella enteriditis phage types had efficacy to control mortality in specific pathogen-free chickens.…”

Section: Discussionmentioning

confidence: 99%

“…However, El-Shall et al [13] reported that the use of live Salmonella enteriditis vaccine had a strong protective effect against Salmonella enteriditis challenge especially for growth performance including body weight gain, which supported the suggestion of inactivated Salmonella enteriditis phage types 3A and 6A for the control of Salmonella enteriditis infection in Malaysia. One major concern in the strategies for the control of Salmonella enteriditis was the colonization of the microbe in different organs such as oviduct, ovaries, liver, spleen, intestine, caecum, and hence, the faecal shedding [12]. e Salmonella isolation results indicated a promising efficacy of inactivated Salmonella enteriditis phage types.…”

Section: Discussionmentioning

confidence: 99%

“…One major concern in the strategies for the control of Salmonella enteriditis was the colonization of the microbe in different organs such as oviduct, ovaries, liver, spleen, intestine, caecum, and hence, the faecal shedding [ 12 ]. The Salmonella isolation results indicated a promising efficacy of inactivated Salmonella enteriditis phage types.…”

Section: Discussionmentioning

confidence: 99%

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Inactivation of Different Salmonella enteriditis Phage Types and Safety and Efficacy of Inactivated Products in Chicken

Akhtar

Hair-Bejo

Hussein

et al. 2021

Veterinary Medicine International

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This study was conducted to inactivate Salmonella enteriditis phage types (SE pt) and to determine the safety and efficacy of inactivated SE pt in chickens. SE pt 1, 3A, 6A, 7, and 35 were inactivated and inoculated (0.20 mL) in 124 chickens divided into 6 groups (CV1, CV3A, CV6A, CV7, CV35, and CV0 as a control). Sampling was conducted on day 14 after inoculation (pi). Eight chickens from each group were separated on day 14 pi for oral challenge with 0.20 mL/chicken (1010 cfu/mL) SE pt 6A and designated CV1C, CV3AC, CV6AC, CV7C, CV35C, and CV0C as control chickens. On days 7 and 14 postchallenge (pc), 4 chickens from every group were sacrificed for sampling. There was no significant difference in the body weight between different groups. In challenged groups, there was no significant association between different tissues and isolation of Salmonella on days 7 and 14 pc. There was significance (p < 0.05) in isolation of Salmonella when CV0C group was compared with other challenged groups. Significance was not observed between different tissues with respect to induction of microscopic changes. Significance was not observed between day 7 pc and day 14 pc with respect to scoring of lesions induced. Clinical signs and gross lesions were also recorded. ELISA was applied. Only in CV3AC group, the mean antibody titer was 1359 on day 14 pc. The conclusion was that inactivated SE pt 3A and 6A were safe and efficacious for protection against Salmonella enteriditis infection in chickens.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Inactivation of Different Salmonella enteriditis Phage Types and Safety and Efficacy of Inactivated Products in Chicken

Akhtar

Hair-Bejo

Hussein

et al. 2021

Veterinary Medicine International

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“…Разработаны сыворотка антитоксическая поливалентная против сальмоноллеза, изготовленная на основе иммуноглобулинов и антитоксинов, полученных из крови животных, гипериммунизированных штаммами сальмонелл; сыворотка антиадгезивная антитоксическая против эшерихиоза сельскохозяйственных животных, изготовленная из крови волов-продуцентов, гипериммунизированных инактивированным антигеном E. сoli [20].…”

Section: Discussionunclassified

Epizootological monitoring of infectious diseases in sheep and goats

et al. 2021

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ЭПИЗООТОЛОГИЯЭпизоотологический мониторинг инфекционной патологии овец и коз РЕЗЮМЕ Актуальность. Установлено достоверное возрастание доли факторных инфекционных болезней, индекс эпизоотичности при которых достигает 1,0, коэффициент очаговости -18,97, летальность -100,0%, недостаточная эффективность диагностических и противоэпизоотических мероприятий обусловливают наличие сопряженных очагов и угрозы формирования энзоотичной зоны. Широкому распространению указанных патологий способствуют снижение естественной резистентности организма животных при содержании на ограниченных площадях, нетрадиционном кормлении и использовании химиотерапевтических и дезинфицирующих средств. Целью работы являлось проведение эпизоотологического мониторинга инфекционной патологии овец на основе оптимизации схемы бактериологической и микологической диагностики. Методы. Объектом исследования явились овцы породы «Романовская», козы породы «Зааненская», ягнята породы «Агинская». При ретроспективном анализе эпизоотической ситуации по инфекционным болезням учитывали статистические данные, оценивая экстенсивные и интенсивные показатели эпизоотического процесса. Для прижизненной диагностики инфекционных болезней исследовали смывы со слизистых оболочек ротовой и носовой полости (n = 10), feces (n = 35) больных ягнят. Кроме того, исследовали смывы объектов репродукторных помещений (n = 5). Pезультаты экспериментальных данных обрабатывали методом статистического анализа с использованием критерия достоверности Стьюдента, результаты считали достоверными при р ≤ 0,05. Результаты. Анализируя данные ветеринарной отчетности, систематизации и статистической обработки эпизоотологических показателей, установили, что из общего числа овец и коз (144 274) число больных животных бактериальными болезнями составило 35, из которых пастереллез -5,72%, эшерихиоз -60,0%, стафилококкоз -2,86%, стрептококкоз -22,86%. В структуре инфекционной патологии овец и коз за период 2014-2018 годы прослеживается динамика изменения нозологического профиля с преобладанием доли эшерихиоза -22,86% и 20,0% в 2014 и 2015 гг. соответственно. Этиологическая структура инфекционной патологии ягнят представлена бактериями E. coli; K. pneumoniae -4 (10,5%); P. vulgaris -3 (7,9%); E. cloacae -2 (5,3%); P. aeruginosa -1 (2,7%); S. aureus -1 (2,7%); S. epidermidis -1 (2,7%); C. albicans -2 (5,3%); C. parapsilosis -1 (2,7%). Epizootological monitoring of infectious diseases in sheep and goats ABSTRACTRelevance. A significant increase in the proportion of factorial infectious diseases was established, the epizootic index in which reaches 1,0, the focal coefficient -18,97, mortality -100,0%, the insufficient effectiveness of diagnostic and antiepizootic measures determine the presence of conjugate foci and the threat of the formation of an enzootic zone. The widespread occurrence of these pathologies is facilitated by a decrease in the natural resistance of the body of animals when kept in limited areas, unconventional feeding and the use of chemotherapeutic and disinfectants. The aim of the work ...

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“…Разработаны способы подготовки препаратов для оптической и сканирующей электронной микроскопии, позволяющие оценить степень образования и морфологический состав биопленок, без нарушения естественной архитектоники популяций микроорганизмов (Ленченко, 2000). При фазово-контрастной микроскопии выявлены процессы, в зависимости от состава среды и содержания кислорода в среде культивирования, установлена агрегация подвижных клеток, вместе с тем, клетки мутантов, лишенные полярных жгутиков, не обладали способностью к подобной быстрой агрегации (Crouch, 2020). При конфокальной микроскопии трехмерные изображения биопленок, позволяют выявить структуру биоплёнки, динамику и скорость процесса осаждения клеток.…”

Section: Introductionunclassified

Study of enterobacteria biofilms critical point control technologies livestock and food production

Lenchenko

Blumenkrants

2021

jour

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The relevance of the study and the presence of gaps in the existing knowledge on the topic. Monitoring studies of the biological safety of food raw materials for microbiological indicators is an urgent problem due to the increase in the number registered diseases transmitted to humans through raw materials and products of animal origin. There is a tendency for a statistically significant increase in epidemiological indicators throughout the world, the proportion of these pathologies is increasing both in humane medicine and veterinary medicine. The aim of the work is a comparative assessment and selection of effective methods for studying the formation biofilms enterobacteriaceae circulating among susceptible animal species and isolated from food raw materials.Methods. Аnalysis of growth and dynamics development biofilms Enterobacteriaceae was carried out during cultivation on nutrient media containing growth factors for the repair of the cell wall and the reversal viable uncultured microorganisms. To study the morphological and functional patterns of the development a population microorganisms in vitro and in vivo, we used the conventional and developed methods for preparing preparations for scanning, transmission phase contrast, optical and luminescence microscopy.Results and its discussion. During microbiological control critical points in the technology of animal husbandry and food production, the morphological and functional characteristics biofilms, which are communities microorganisms secreting a polymer matrix and adhered to the tissues of susceptible animal species and abiotic surfaces livestock buildings and food industries, were studied. The developed methods of biofilm cultivation made it possible to study enterobacteriaceae biofilms in vitro and in vivo, without disturbing the natural architectonics of the population microorganisms, to determine the components extracellular matrix. For the study dynamics morphological and functional patterns of the development populations microorganisms, routine and technological advances present are recognized as promising, for example, scanning electron microscopy makes it possible to assess the degree of formation and morphological composition biofilms. Phase contrast microscopy to reveal processes depending on the composition medium and the oxygen content in the culture medium.Conclusions. Methods for cultivating biofilms in vitro and in vivo without disturbing the natural architectonics of biofilms made it possible to optimize the preparation samples for research and eliminate the routine stages of colony counting, and significantly increase the number of analyzes. Due to the simplicity of operations and minimization manual labor, productivity increases, safety of work is increased, cost of personnel working time is reduced, and subjective factors are excluded. For the development of a complex antiepizootic and diagnostic measures, a priority direction is the disclosure of scientific knowledge in the field fundamental studies of ecological plasticity and adaptation potentially pathogenic enterobacteria to parasitism in the warm-blooded organism of birds and mammals. This will allow solving applied problems controlling the critical points of livestock and food production technology, developing effective chemotherapeutic and disinfecting drugs to reduce cell coaggregation and detect viable uncultured microorganisms.

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Safety and efficacy of a novel inactivated trivalent Salmonella enterica vaccine in chickens

Cited by 23 publications

References 33 publications

Inactivation of Different Salmonella enteriditis Phage Types and Safety and Efficacy of Inactivated Products in Chicken

Inactivation of Different Salmonella enteriditis Phage Types and Safety and Efficacy of Inactivated Products in Chicken

Epizootological monitoring of infectious diseases in sheep and goats

Study of enterobacteria biofilms critical point control technologies livestock and food production

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