Safety and efficacy of living donor liver preservation with HTK solution

Ringe, Burckhardt; Braun, Felix; Moritz, Michael J.; Zeldin, Gillian; Soriano, Humberto E.; Meyers, William C.

doi:10.1016/j.transproceed.2004.12.009

Cited by 23 publications

(8 citation statements)

References 22 publications

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“…This was done to prevent leakage of perfusate and to maximize perfusion of the microcirculation during isolation, having been previously shown to increase hepatocyte yield and viability [14,15] . The specimen was then flushed with 500-1000 mL of heparinized (5000 units per litre) Custodiol ® histidine-tryptophan-ketoglutarate (HTK) solution (Kohler Chemie, Ger many) [16] to prevent obstruction of the hepatic microcirculation by thrombus and facilitate organ preservation. The specimen was then transported under sterile conditions to the laboratory where it was re-warmed and digested by a modified version of Seglen's original 2-step technique [17] .…”

Section: Hepatocyte Isolation and Cryopreservationmentioning

confidence: 99%

Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation

Gunasegaram¹,

Akhter²,

Yao³

et al. 2008

WJG

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AIM:To investigate whether hepatocytes isolated from macroscopically normal liver during hepatic resection for neoplasia could provide a novel source of healthy hepatocytes, including the development of reliable protocols for malignant cells removal from the hepatocyte preparation. METHODS: Hepatocytes were procured from resected liver of 18 patients with liver tumors using optimised digestion and cell-enrichment protocols. Suspensions of various known quantities of the HT-29 tumor cell line and patient hepatocytes were treated or not with Ep-CAM-antibody-coated immunomagnetic beads in order to investigate the efficacy of tumor-purging by immunomagnetic depletion, using a semi-quantitative RT-PCR method developed to detect tumor cells. Immunomagnetic bead-treated or bead-untreated tumor cell-hepatocyte suspensions were transplanted intra-peritoneally in Balb/C nude mice to assess the rates of tumor development. RESULTS: Mean viable hepatocyte yield was 9.

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Section: Hepatocyte Isolation and Cryopreservationmentioning

confidence: 99%

Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation

Gunasegaram¹,

Akhter²,

Yao³

et al. 2008

WJG

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“…The second possible advantage of HTK lies in the solution′s low potassium concentration, which precipitated HTK′s widespread use and popularity . The postreperfusion syndrome (PRS) in LT with intense hemodynamic instability is partially attributed to the large amount of potassium that can be effused into the systemic circulation during graft reperfusion.…”

Section: Introductionmentioning

confidence: 99%

Graft rinse prior to reperfusion in liver transplantation: literature review and online survey within the Eurotransplant community

et al. 2015

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SummaryGraft rinse prior reperfusion in liver transplantation (LT) is believed to reduce the incidence of postreperfusion syndrome and improve clinical outcome. A MEDLINE search was performed to obtain a comprehensive review of the published literature dealing with graft rinse in LT. Moreover, all thirty-four LT centers in the Eurotransplant (ET) region were invited to participate in an online survey to whether or not graft rinse is performed and whether further research in the field is needed. Seventeen reports have been found to investigate graft rinse protocols in 1894 LT recipients. Eighteen of the thirty centers that participated in the online survey performed graft rinse prior reperfusion in LT. The most commonly used rinse solution was albumin. Nineteen centers stated interest in participating in a multicenter RCT in the field. The published literature does not provide concluding appraisal of the benefit of graft rinse in LT. Graft rinse protocols are not standardized and are based on personal experience. Appropriately designed clinical trials addressing the topic are demanded. The online survey appears to be a helpful tool for the evaluation of clinical practice and future research topics in the transplant community.

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“…These solutions are generally composed of electrolytes and buffers, with carbohydrates, such as glucose and trehalose, added to prevent energy loss and increase the osmotic pressure of the solution. In addition, to inhibit the generation of reactive oxygen species during cold preservation and ischemia/reperfusion, antioxidants, glutathione and N-acetylcysteine, are added to the University of Wisconsin solution and ET-Kyoto solution, respectively [3, 4]. Although these solutions are essential for clinical transplantation, they have several disadvantages, including chemical instability and high viscosity.…”

Section: Introductionmentioning

confidence: 99%

Comparative Effects of Phosphoenolpyruvate, a Glycolytic Intermediate, as an Organ Preservation Agent with Glucose and N-Acetylcysteine against Organ Damage during Cold Storage of Mouse Liver and Kidney

et al. 2013

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We evaluated the usefulness of phosphoenolpyruvate (PEP), a glycolytic intermediate with antioxidative and energy supplementation potentials, as an organ preservation agent. Using ex vivo mouse liver and kidney of a static cold storage model, we compared the effects of PEP against organ damage and oxidative stress during cold preservation with those of glucose or N-acetylcysteine (NAC). Lactate dehydrogenase (LDH) leakage, histological changes, and oxidative stress parameters (measured as thiobarbituric acid reactive substance and glutathione content) were determined. PEP (100 mM) significantly prevented an increase in LDH leakage, histological changes, such as tubulonecrosis and vacuolization, and changes in oxidative stress parameters during 72 h of cold preservation in mouse liver. Although glucose (100 mM) partly prevented LDH leakage and histological changes, no effects against oxidative stress were observed. By contrast, NAC inhibited oxidative stress in the liver and did not prevent LDH leakage or histological changes. PEP also significantly prevented kidney damage during cold preservation in a dose-dependent manner, and the protective effects were superior to those of glucose and NAC. We suggest that PEP, a functional carbohydrate with organ protective and antioxidative activities, may be useful as an organ preservation agent in clinical transplantation.

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Safety and efficacy of living donor liver preservation with HTK solution

Cited by 23 publications

References 22 publications

Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation

Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation

Graft rinse prior to reperfusion in liver transplantation: literature review and online survey within the Eurotransplant community

Comparative Effects of Phosphoenolpyruvate, a Glycolytic Intermediate, as an Organ Preservation Agent with Glucose and N-Acetylcysteine against Organ Damage during Cold Storage of Mouse Liver and Kidney

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