Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review

Dini, Theresia; Nugraha, Yudhi; Revina, Rika; Karina, Karina

doi:10.21705/mcbs.v6i3.252

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…(23) Both Bax (pro-apoptosis) and Bcl-2 (anti-apoptosis) controls the mitochondrial movement during cell death stimulation and influences the immune cells. (25,26) Results of the present study showed that CCLEtreated group decreased Bcl-2 protein content of HSC-3 cells (Figure 4) and increased Bax protein content of HSC-3 cells (Figure 5) in concentration-dependent manner. Since quercetin was the mainly found flavonoids in the leaves of C. caudatus (5), the quercetin could have the potential activity in affecting the Bcl-2 and Bax protein contents.…”

Section: Discussionmentioning

confidence: 53%

Cosmos caudatus Leaf Extract Triggers Apoptosis of HSC-3 Cancer Cells by Decreasing Bcl-2 and Increasing Bax

Sandra,

Rizal,

Dhaniar

et al. 2024

Indones Biomed J

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BACKGROUND: Previous studies have demonstrated that Cosmos caudatus leaf extract (CCLE) exhibits cytotoxic effects against various types of human cancer. However, the CCLE cytotoxic effect towards oral squamous cell carcinoma (OSCC) cells has not been investigated. Therefore, this study was conducted to evaluate the effect of CCLE towards the viability and apoptosis in human oral squamous carcinoma (HSC)-3 cells.METHODS: HSC-3 cells were treated with various concentrations of CCLE for 24 h. The number of viable HSC-3 cells were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), meanwhile the apoptotic HSC-3 cells were measured using sub-G1 assay. Mitochondrial membrane potential was measured using flow cytometry. Bcl-2 and Bax protein content of HSC-3 cells were measured using enzyme-linked immunosorbent assay (ELISA).RESULTS: CCLE treatment could decrease the number of HSC-3 viable cells and increase the percentage of HSC-3 apoptotic cells in concentration-dependent manner. In mitochondrial membrane potential assay, CCLE-treated group displayed a peak shifment from 104 to 103. Bcl-2 protein contents of CCLE-treated group were decrease in concentration-dependent manner, meanwhile Bax protein contents of CCLE-treated group were increase in concentration-dependent manner.CONCLUSION: CCLE could trigger apoptosis in HSC-3 cells by decreasing Bcl-2 protein content and increasing Bax protein content in concentration-dependent manner, leading to intrinsic apoptotic pathway.KEYWORDS: Cosmos caudatus, HSC-3, apoptosis, mitochondrial membrane potential, Bcl-2, Bax

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Section: Discussionmentioning

confidence: 53%

Cosmos caudatus Leaf Extract Triggers Apoptosis of HSC-3 Cancer Cells by Decreasing Bcl-2 and Increasing Bax

Sandra,

Rizal,

Dhaniar

et al. 2024

Indones Biomed J

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“…1 One of the sources of MSCs, which has been utilized in investigations and treatments relatively frequently up to this point, is the umbilical cord. The effectiveness of umbilical cord MSCs as a treatment for heart failure 2 , periapical periodontitis 3 , rheumatoid arthritis 4 , numerous studies of burn cases 5 , and several studies to treat Coronavirus disease 2019 (COVID-19). 6 MSCs have to maintain its function and act as intended while treating degenerative disorders to be therapeutically effective.…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cell in 3D Culture: Diminishing Cell Senescence in Cryopreservation and Long-term Expansion

Jundan,

Amalia,

Sartika

2023

Mol Cell Biomed Sci

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Mesenchymal stem cells (MSCs) are widely recognized in cell treatment due to their capacity to secrete trophic factors, differentiate multipotent, and self-renew. Although there is growing evidence that MSCs have therapeutic benefits in various clinical settings, these cells eventually lose their ability to regenerate as they age, which increases cellular dysfunction. Several factors may affect MSCs aging, such as culture dimensions, cryopreservation process, and long-term expansion. Traditional two-dimensional (2D) culture conditions lack the complexities required to recreate MSCs in their natural environment. Meanwhile, three-dimensional (3D) culture mimics the niche, dynamic, and specialized microenvironments of the cells in vivo. The most used storage technique for MSCs, cryopreservation, requires a very low temperature reduction, which stresses cells and can cause the release of pro-inflammatory cytokines. For the utilization of MSCs in therapeutic applications, an in vitro expansion technique is required. Repeated expansion may reduce proliferative capacity, disrupts cellular shape, and impairs the somatic cell function of MSCs. Various processes and techniques may influence MSCs leading to cell aging. One of the culture methods, 3D culture, is shown to reduce the factors that will compromise the therapeutic effects of MSCs, especially cell senescence. The effect of culture dimensions, cryopreservation, and long-term expansion on cell senescence will be discussed in this review article.Keywords: cell aging, mesenchymal stem cell, 3D culture, cell senescence, cryopreservation, long-term expansion

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Exploring the therapeutic potential of different sources of mesenchymal stem cells: a novel approach to combat burn wound infections

Aliniay-Sharafshadehi,

Yousefi,

Ghodratie

et al. 2024

Front. Microbiol.

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The most prevalent and harmful injuries are burns, which are still a major global health problem. Burn injuries can cause issues because they boost the inflammatory and metabolic response, which can cause organ malfunction and systemic failure. On the other hand, a burn wound infection creates an environment that is conducive to the growth of bacteria and might put the patient at risk for sepsis. In addition, scarring is unavoidable, and this results in patients having functional and cosmetic issues. Wound healing is an amazing phenomenon with a complex mechanism that deals with different types of cells and biomolecules. Cell therapy using stem cells is one of the most challenging treatment methods that accelerates the healing of burn wounds. Since 2000, the use of mesenchymal stem cells (MSCs) in regenerative medicine and wound healing has increased. They can be extracted from various tissues, such as bone marrow, fat, the umbilical cord, and the amniotic membrane. According to studies, stem cell therapy for burn wounds increases angiogenesis, has anti-inflammatory properties, slows the progression of fibrosis, and has an excellent ability to differentiate and regenerate damaged tissue. Figuring out the main preclinical and clinical problems that stop people from using MSCs and then suggesting the right ways to improve therapy could help show the benefits of MSCs and move stem cell-based therapy forward. This review’s objective was to assess mesenchymal stem cell therapy’s contribution to the promotion of burn wound healing.

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Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review

Cited by 3 publications

References 43 publications

Cosmos caudatus Leaf Extract Triggers Apoptosis of HSC-3 Cancer Cells by Decreasing Bcl-2 and Increasing Bax

Cosmos caudatus Leaf Extract Triggers Apoptosis of HSC-3 Cancer Cells by Decreasing Bcl-2 and Increasing Bax

Mesenchymal Stem Cell in 3D Culture: Diminishing Cell Senescence in Cryopreservation and Long-term Expansion

Exploring the therapeutic potential of different sources of mesenchymal stem cells: a novel approach to combat burn wound infections

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