Safety and Feasibility of Contrast-Enhanced Computed Tomography with a Nanoparticle Contrast Agent for Evaluation of Diethylnitrosamine-Induced Liver Tumors in a Rat Model

Nota, Takehito; Kageyama, Ken; Yamamoto, Akira; Kakehashi, Anna; Yonezawa, Hiroki; Jogo, Atsushi; Sohgawa, Etsuji; Murai, Kazuki; Ogawa, Satoyuki; Miki, Yukio

doi:10.1016/j.acra.2022.03.027

Cited by 5 publications

(4 citation statements)

References 39 publications

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“…With human anticancer therapy, CT is popularly used for evaluation as most tumors are deep-seated and not externally visible 38 . Nota et al developed a minimally invasive method for evaluating rat liver tumors on contrast-enhanced CT using Exitron nano 12000 contrast agent in a rat liver tumor model 25 . They validated the concordance between CT images and tumor size in pathological specimens.…”

Section: Discussionmentioning

confidence: 99%

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A rat-based preclinical platform facilitating transcatheter hepatic arterial infusion in immunodeficient rats with liver xenografts of patient-derived pancreatic ductal adenocarcinoma

Ozaki,

Kageyama,

Kimura

et al. 2024

Sci Rep

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Liver metastases from pancreatic ductal adenocarcinoma (PDAC) are highly fatal. A rat-based patient-derived tumor xenograft (PDX) model is available for transcatheter therapy. This study aimed to create an immunodeficient rat model with liver xenografts of patient-derived primary PDAC and evaluate efficacy of hepatic arterial infusion chemotherapy with cisplatin in this model. Three patient-derived PDACs were transplanted into the livers of 21 rats each (totally, 63 rats), randomly assigned into hepatic arterial infusion, systemic venous infusion, and control groups (n = 7 each) four weeks post-implantation. Computed tomography evaluated tumor volumes before and four weeks after treatment. Post-euthanasia, resected tumor specimens underwent histopathological examination. A liver-implanted PDAC PDX rat model was established in all 63 rats, with first CT identifying all tumors. Four weeks post-treatment, arterial infusion groups exhibited significantly smaller tumor volumes than controls for all three tumors on second CT. Xenograft tumors histologically maintained adenocarcinoma features compared to original patient tumors. Ki67 expression was significantly lower in arterial infusion groups than in the other two for the three tumors, indicating reduced tumor growth in PDX rats. A liver-implanted PDAC PDX rat model was established as a rat-based preclinical platform. Arterial cisplatin infusion chemotherapy represents a potential therapy for PDAC liver metastasis.

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Section: Discussionmentioning

confidence: 99%

“…Liver tissues and liver tumors in rats were visualized on contrast-enhanced micro-CT using contrast agent (Viscover ExiTron nano 12000, Miltenyi Biotec, Bergisch-Gladbach, Germany) 25 . This contrast agent is an alkaline earth-based nanoparticulate contrast agent specifically formulated for preclinical CT 26 .…”

Section: Methodsmentioning

confidence: 99%

A rat-based preclinical platform facilitating transcatheter hepatic arterial infusion in immunodeficient rats with liver xenografts of patient-derived pancreatic ductal adenocarcinoma

Ozaki,

Kageyama,

Kimura

et al. 2024

Sci Rep

Self Cite

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“…The use of MRI images in rats has a strong impact on the result verification. In addition, using other modalities of tumor investigations such as Computed Tomography (CT) and combined images of (CT) & Positron Emission Tomography (PET-CT) in rats are also still used in research [32,33]. Here, estimating brain damage (as mass, inflammation, even tumor tissue) of rats due to exposure to TE-NORM is our interest in this research which may be limited in the literature.…”

Section: Mri Analysismentioning

confidence: 99%

Brain Imaging and Hematological Evaluation of the Risk of Frequent Exposure to Naturally Occurring Radioactive Materials From Petroleum Oil Fields in Rats

El Bana,

Abdel Gawad,

Eltahawy

et al. 2023

Egypt. J. Biophys. Biomed. Engng.

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“…Moreover, a novel approach was introduced to advance ART technology for other lower-resolution tumors in CT images. Previous investigations have demonstrated the accuracy of nanoreagent distribution and imaging in liver tumors. − However, the study focused on designing a nano-CT-enhanced contrast agent for prolonged imaging in sHCC and establishing its efficacy in automatic segmentation for AI. The study had limitations, such as the unvalidated imaging accuracy of sHCC using the charged AuNP contrast agent and the limited data set for training the deep learning-based segmentation model.…”

mentioning

confidence: 99%

Charged Gold Nanoparticles for Target Identification–Alignment and Automatic Segmentation of CT Image-Guided Adaptive Radiotherapy in Small Hepatocellular Carcinoma

Lai,

Luo,

Liu

et al. 2024

Nano Lett.

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Because of the challenges posed by anatomical uncertainties and the low resolution of plain computed tomography (CT) scans, implementing adaptive radiotherapy (ART) for small hepatocellular carcinoma (sHCC) using artificial intelligence (AI) faces obstacles in tumor identification−alignment and automatic segmentation. The current study aims to improve sHCC imaging for ART using a gold nanoparticle (Au NP)-based CT contrast agent to enhance AI-driven automated image processing. The synthesized charged Au NPs demonstrated notable in vitro aggregation, low cytotoxicity, and minimal organ toxicity. Over time, an in situ sHCC mouse model was established for in vivo CT imaging at multiple time points. The enhanced CT images processed using 3D U-Net and 3D Trans U-Net AI models demonstrated high geometric and dosimetric accuracy. Therefore, charged Au NPs enable accurate and automatic sHCC segmentation in CT images using classical AI models, potentially addressing the technical challenges related to tumor identification, alignment, and automatic segmentation in CT-guided online ART.

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Safety and Feasibility of Contrast-Enhanced Computed Tomography with a Nanoparticle Contrast Agent for Evaluation of Diethylnitrosamine-Induced Liver Tumors in a Rat Model

Cited by 5 publications

References 39 publications

A rat-based preclinical platform facilitating transcatheter hepatic arterial infusion in immunodeficient rats with liver xenografts of patient-derived pancreatic ductal adenocarcinoma

A rat-based preclinical platform facilitating transcatheter hepatic arterial infusion in immunodeficient rats with liver xenografts of patient-derived pancreatic ductal adenocarcinoma

Brain Imaging and Hematological Evaluation of the Risk of Frequent Exposure to Naturally Occurring Radioactive Materials From Petroleum Oil Fields in Rats

Charged Gold Nanoparticles for Target Identification–Alignment and Automatic Segmentation of CT Image-Guided Adaptive Radiotherapy in Small Hepatocellular Carcinoma

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