Safety and Quality in the Agricultural Product Chain in Brazil

Abstract

Abstract"n agriculture-intensive country should be aware of natural toxins, including both mycotoxins and cyanotoxins, which are closely associated with the quality of raw materials, for food safety and industry. The major production chains -corn, wheat, beef, and broiler chicken -are the top components of agribusiness, and they should be tracked by reliable and practical tools. The corn chain is of particular concern in food production intensive controls, multi-year mycotoxin monitoring, and improved harmless/sustainable management methods for uninterrupted farming in the tropicsubtropics are needed to achieve a long-lasting trend. The rapid control of natural toxins mycotoxin and cyanotoxin has focused on immunochemical methods developed with highly specific monoclonal antibodies m"b matched with chromatographic methods. In parallel, the promising widespread application of nondestructive analytical methods based on NIR Near Infrared Reflectance

Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain

“…This undesired interference can be minimized by simple sample dilution, or by including adequate cleaning-up steps prior to the analysis. However, the dependence on expensive imported commercial kits discourages the constant repetitive analysis of the same sample or its dilutions in the field of agroindustry (Ono et al, 2004;Hirooka et al, 2015).…”

“…The anti-aflatoxin monoclonal antibody (mAb) provides unrestricted possibilities for the development of quantitative or semiquantitative assays such as Elisa intended for monitoring aflatoxins in food products. These immunochemistry-based methods employing mAb offer high-sensitivity and adequate limits of detection/quantification, in comparison with HPLC-based methods, and can be applied to different agricultural raw materials to detect the presence of toxins (Hirooka et al, 2015).…”

Comparação entre LABicELISA e kit ELISA comercial para análise de microcistinas em águas

“…LABicELISA é um ELISA competitivo indireto que empregou AcM anti-MC-LR produzido in vitro pela linhagem de hibridoma MC.5-3, com reatividade cruzada de 146% para MC-RR e 88% para MC-YR (HIROOKA et al, 2015;TABUCHI et al, 2015). O LABicELISA foi realizado seguindo o protocolo descrito por Nagata et al (1997) com adaptações.…”

“…Uma das dificuldades na análise de MCs em países em desenvolvimento, como o Brasil, é a aquisição, operação e manutenção de instrumentos sofisticados de alto custo como sistemas cromatográficos, bem como o uso de kits de imunoensaio importados, resultando também em custos elevados na análise. Sendo assim, uma das alternativas seria empregar imunoensaio ELISA desenvolvido a partir da produção ilimitada de AcM específico oriundo de hibridoma com menor custo de análise (HIROOKA et al, 2015;KHREICH et al, 2009;PYO, LEE e CHOI, 2005;SHENG et al, 2006).…”