Safety assessment and probiotic characteristics of Enterococcus lactis JDM1

Xiao-mei, FU; Lyu, Lin; Wang, Yue; Zhang, Yan; Guo, Xia; Chen, Qian; Liu, Chang

doi:10.1016/j.micpath.2021.105380

Cited by 40 publications

(21 citation statements)

References 52 publications

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“…The results indicated that the cell viability of Lactococcus garvieae FUA009 decreased significantly after treatment with acid (pH 2.0) and 0.5% bile salt in turn ( Supplementary Figure S3 ). However, 0.3% bile salt is often used to screen probiotics because the concentration of bile salt in the human intestine can be as high as 0.3% [ 46 , 47 ]. After the acid (pH 2.0 and pH 3.0) treatment, the residual cells of Lactococcus garvieae FUA009 were exposed to 0.3% bile salt and the final cell viabilities were 41% and 48%, respectively ( Supplementary Figure S3 ).…”

Section: Discussionmentioning

confidence: 99%

Lactococcus garvieae FUA009, a Novel Intestinal Bacterium Capable of Producing the Bioactive Metabolite Urolithin A from Ellagic Acid

Mi¹,

Liu

Hai

et al. 2022

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Dietary polyphenol ellagic acid has anti-cancer and anti-inflammatory activities, and these biological activities require the conversion of ellagic acid to urolithins by intestinal microbes. However, few gut microbes are capable of metabolizing ellagic acid to produce urolithins, limiting the beneficial effects of ellagic acid on health. Here, we describe an intestinal bacterium Lactococcus garvieae FUA009 isolated from the feces of a healthy volunteer. It was demonstrated via HPLC and UPLC-MS analysis that the end product of ellagic acid metabolism of FUA009 was urolithin A. In addition, we also examined the whole genome sequence of FUA009 and then assessed the safety and probiotic properties of FUA009 based on a complete genome and phenotype analysis. We indicated that FUA009 was safe, which was confirmed by FUA009 being sensitive to multiple antibiotics, having no hemolytic activity, and being free of aggressive putative virulence factors. Moreover, 19 stress-responsive protein genes and 8 adhesion-related genes were predicted in the FUA009 genome. Furthermore, we demonstrated that FUA009 was tolerant to acid and bile salt by determining the cell viability in a stress environment. In summary, Lactococcus garvieae FUA009, as a novel UA-producing bacterium, not only contributes to the study of the metabolic pathway of ellagic acid but is also expected to be a novel probiotic candidate.

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Section: Discussionmentioning

confidence: 99%

Lactococcus garvieae FUA009, a Novel Intestinal Bacterium Capable of Producing the Bioactive Metabolite Urolithin A from Ellagic Acid

Mi¹,

Liu

Hai

et al. 2022

Foods

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“…Virulence factors and resistance genes were identified based on the core datasets in the VFDB (Virulence Factors of Pathogenic Bacteria, , accessed on 26 May 2022) and CARD (Comprehensive Antibiotic Resistance Database, , accessed on 26 May 2022) databases, with coverage > 60% and identity > 75% [ 13 ]. Genes involved in the production of toxins were searched in the PGAP annotation results of the X253 and GG genomes.…”

Section: Methodsmentioning

confidence: 99%

“…Probiotics exhibit strain-specific properties with regard to their safety and function [ 12 ]. It has been hypothesized that improper administration of probiotics may result in four kinds of detrimental effects: systemic infection, creation of toxic metabolites, excessive immunological activation, and transfer of antibiotic resistance and virulence genes [ 13 ]. Consequently, it is necessary to confirm the safety and probiotic characteristics of novel isolates prior to evaluating their therapeutic advantages and incorporating them into foods and pharmaceuticals [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Assessing the Safety and Probiotic Characteristics of Lacticaseibacillus rhamnosus X253 via Complete Genome and Phenotype Analysis

et al. 2023

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Lacticaseibacillus rhamnosus is a generalist that can adapt to different ecological niches, serving as a valuable source of probiotics. The genome of L. rhamnosus X253 contains one chromosome and no plasmids, with a size of 2.99 Mb. Both single-copy orthologous gene-based phylogenetic analysis and average nucleotide identity indicated that dairy-derived L. rhamnosus X253 was most closely related to the human-intestine-derived strain L. rhamnosus LOCK908, rather than other dairy strains. The adaptation of L. rhamnosus X253 and the human-intestine-derived strain L. rhamnosus GG to different ecological niches was explained by structural variation analysis and COG annotation. Hemolytic assays, API ZYM assays, and antimicrobial susceptibility tests were performed to validate risk-related sequences such as virulence factors, toxin-encoding genes, and antibiotic-resistance genes in the genomes of L. rhamnosus X253 and GG. The results showed that L. rhamnosus GG was able to use L-fucose, had a higher tolerance to bile salt, and adhered better to CaCo-2 cells. In contrast, L. rhamnosus X253 was capable of utilizing D-lactose, withstood larger quantities of hydrogen peroxide, and possessed excellent antioxidant properties. This study confirmed the safety and probiotic properties of L. rhamnosus X253 via complete genome and phenotype analysis, suggesting its potential as a probiotic.

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“…For example, they are recommended in the treatment of serum cholesterol (E. faecium M-74) [77], antibiotic-associated diarrhea (E. faecium SF68) [78], and irritable bowel syndrome (E. faecalis DSM 16440) [79]. Moreover, a very recent study of the newly isolated Enterococcus lactis strain JDM1 assessed its probiotic potential and good gastrointestinal tolerance [80].…”

Section: Shifts Observed In the Bacterial Community Composition Follo...mentioning

confidence: 99%

Human Adult Microbiota in a Static Colon Model: AhR Transcriptional Activity at the Crossroads of Host–Microbe Interaction

Goya-Jorge

Gonza

Bondue³

et al. 2022

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Functional symbiotic intestinal microbiota regulates immune defense and the metabolic processing of xenobiotics in the host. The aryl hydrocarbon receptor (AhR) is one of the transcription factors mediating host–microbe interaction. An in vitro static simulation of the human colon was used in this work to analyze the evolution of bacterial populations, the microbial metabolic output, and the potential induction of AhR transcriptional activity in healthy gut ecosystems. Fifteen target taxa were explored by qPCR, and the metabolic content was chromatographically profiled using SPME-GC-MS and UPLC-FLD to quantify short-chain fatty acids (SCFA) and biogenic amines, respectively. Over 72 h of fermentation, the microbiota and most produced metabolites remained stable. Fermentation supernatant induced AhR transcription in two of the three reporter gene cell lines (T47D, HepG2, HT29) evaluated. Mammary and intestinal cells were more sensitive to microbiota metabolic production, which showed greater AhR agonism than the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) used as a positive control. Some of the SCFA and biogenic amines identified could crucially contribute to the potent AhR induction of the fermentation products. As a fundamental pathway mediating human intestinal homeostasis and as a sensor for several microbial metabolites, AhR activation might be a useful endpoint to include in studies of the gut microbiota.

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Safety assessment and probiotic characteristics of Enterococcus lactis JDM1

Cited by 40 publications

References 52 publications

Lactococcus garvieae FUA009, a Novel Intestinal Bacterium Capable of Producing the Bioactive Metabolite Urolithin A from Ellagic Acid

Lactococcus garvieae FUA009, a Novel Intestinal Bacterium Capable of Producing the Bioactive Metabolite Urolithin A from Ellagic Acid

Assessing the Safety and Probiotic Characteristics of Lacticaseibacillus rhamnosus X253 via Complete Genome and Phenotype Analysis

Human Adult Microbiota in a Static Colon Model: AhR Transcriptional Activity at the Crossroads of Host–Microbe Interaction

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