Safety evaluation and bioassay-guided isolation of antimycobacterial compounds from Morella salicifolia root ethanolic extract

Marealle, Alphonce Ignace; Innocent, Ester; Andrae‐Marobela, Kerstin; Qwarse, Michael; Machumi, Francis; Nondo, Ramadhani S O; Heydenreich, Matthias; Moshi, Mainen J.

doi:10.1016/j.jep.2022.115501

Cited by 8 publications

(2 citation statements)

References 38 publications

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“…Marealle et al reported the antimycobacterial activity of compounds isolated from M. salicifolia against three nonpathogenic mycobacterial species. Maslinic acid showed the highest MICs value of 17, 28, and 56 μg/mL against Mycobacterium madagascariense, standard Mycobacterium tuberculosis strain H 37 RV, and rifampicin-resistant M. tuberculosis clinical isolates, respectively [9]. It has been linked to antimalarial [4], antiplasmodial [10], strong analgesic efects [11], and lowering blood glucose efects [12].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antioxidant Activities of Triterpenoids and Cyclic 1,7-Diarylheptanoids from the Stem Bark of Myrica salicifolia: A Combined Experimental and Computational Study

Gashaw,

Bedane,

Demissie

et al. 2024

Journal of Chemistry

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Myricasalicifolia A Rich (Myricaceae) is a tree growing in Central and East Africa. Traditionally, the plant is used to treat malaria, respiratory disorders, inflammations, and infections. A new compound, 3β-O-trans-caffeoylisomyricadiol (7), was isolated from MeOH : CHCl3 (2 : 1) extract of the stem bark of Myrica salicifolia along with seven known compounds, namely, myricanone (1), myricanol (2), myricanol-11-O-β-D-xylopyranoside (3), taraxerone (4), taraxerol (5), myricadiol (6), and methyl-β-D-glucopyranoside (8). This is the first report of the isolation of taraxerene-type triterpenes from this plant. The structures were determined by a comprehensive analysis of 1D/2D NMR spectroscopy, HR-MS, and by comparison with literature data. The compounds showed a wide range of DPPH scavenging activities from very weak (IC50 value = 282.61 μM) to very strong (IC50 = 13.48 μM). Antibacterial activities of the compounds were evaluated using the disk diffusion agar method, where some of the compounds showed modest antibacterial activities against S. pyogenes and S. aureus at 250 μg/mL. Compounds 2, 3, and 7 were assessed for their in silico molecular docking analysis. The lowest binding affinity for compound 7 was found to be −7.26 to −10.35 kcal/mol against PqsA protein of P. aeruginosa, pyruvate kinase (PK) enzyme of S. aureus, LuxS protein of S. pyogenes, and DNA gyrase B of E. coli, which showed better binding affinity compared to the standard drug ampicillin (−7.36 to −8.03 kcal/mol) and ciprofloxacin (−6.19 to −6.83 kcal/mol). In silico ADMET predictions revealed that compounds 3 and 8 met all the requirements for pharmacokinetic properties.

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Section: Introductionmentioning

confidence: 99%

Antibacterial and Antioxidant Activities of Triterpenoids and Cyclic 1,7-Diarylheptanoids from the Stem Bark of Myrica salicifolia: A Combined Experimental and Computational Study

Gashaw,

Bedane,

Demissie

et al. 2024

Journal of Chemistry

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“…Most studies have shown that plant alkaloids have special physiological activity [3]. The alkaloid active components contained in the Semen Nelumbinis and plumula nelumbinis mainly include Lotusine, Liensinine, Isoliensinine, Neferine, Nuciferine, Pronuciferine, Berberine Hydrochloride and the like [4], which have many health promotion functions and medicinal values for human body, such as anti-cancer, anti-virus, anti-bacteria, anti-arrhythmia, blood pressure reduction, blood glucose reduction, anti-oxidation, and free radical removal [5,6]. As many alkaloid components in Semen Nelumbinis are very important chemical components, which are also important indicators to evaluate the quality of and have great influence on the sensory quality and medicinal value of Semen Nelumbinis, it is of great significance to extract and quantitatively determine alkaloids in Semen Nelumbinis.…”

Section: Introductionmentioning

confidence: 99%

Determination of 7 Kinds of Alkaloids in Semen Nelumbinis and Its Products by HPLC

Zhong

Ouyang

Deng³

et al. 2022

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Objective: To establish a method for the simultaneous determination of seven alkaloids in Semen Nelumbinis and its products, the extraction technology and HPLC method were optimized by the single factor experiment. Methods: Firstly, the samples were extracted ultrasonically with 1% formic acid ethanol and purified by PXC SPE column. Then, the extracts and the purified liquid were taken after concentration with nitrogen for quantitative analysis of seven alkaloids by HPLC method. Next, the contents of alkaloids in five samples were determined. Results: The method was fully validated and the result showed that seven kinds of alkaloids had good linear relation in the corresponding range of mass concentration, r2 > 0.999, where the detection limit was 0.5–1.5 mg/kg, the quantification limit was 1.25–4.5 mg/kg, the recovery was 83.33–116.04%, and the RSD of detection method was 1.06–5.25% (n = 7). In five samples, the contents of Lotusine and Neferine were the highest, Berberine Hydrochloride was not detected. Conclusion: This method is simple, sensitive, accurate and reproducible, and it can realize the quantitative analysis and chemical separation of seven kinds of common alkaloids in Semen Nelumbinis and its products and provide a theoretical method for the simultaneous determination of alkaloids. The extraction yields of alkaloids in Semen Nelumbinis can be increased through the extraction process, which is optimized by a single factor experiment.

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Exploring the Therapeutic Potential of Wild Edible Mushrooms: Safety Evaluation and Isolation of Antimycobacterial Sterols from Afrocantharellus platyphyllus (Heinem.) Tibuhwa

Qwarse,

Marealle,

Machumi

et al. 2023

Chemistry Africa

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Safety evaluation and bioassay-guided isolation of antimycobacterial compounds from Morella salicifolia root ethanolic extract

Cited by 8 publications

References 38 publications

Antibacterial and Antioxidant Activities of Triterpenoids and Cyclic 1,7-Diarylheptanoids from the Stem Bark of Myrica salicifolia: A Combined Experimental and Computational Study

Antibacterial and Antioxidant Activities of Triterpenoids and Cyclic 1,7-Diarylheptanoids from the Stem Bark of Myrica salicifolia: A Combined Experimental and Computational Study

Determination of 7 Kinds of Alkaloids in Semen Nelumbinis and Its Products by HPLC

Exploring the Therapeutic Potential of Wild Edible Mushrooms: Safety Evaluation and Isolation of Antimycobacterial Sterols from Afrocantharellus platyphyllus (Heinem.) Tibuhwa

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