Safety Evaluation of Enterocin B Producer Enterococcus Faecalis Mye58 Strain Isolated From Raw Milk

Abstract

Özet SAFETY EVALUATION OF ENTEROCIN B PRODUCER ENTEROCOCCUS FAECALIS MYE58 STRAIN ISOLATED FROM RAW MILK AbstractIn this study, we investigated the occurrence of enterocin, virulence factor and vancomycin resistance genes in bacteriocin producer Enterococcus faecalis MYE58 isolated from raw milk. We also tested hemolytic activity, gelatinase production and antibiotic resistance of this strain. It was determined that MYE58 strain inhibited Gram-positive bacteria, such as Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus. The presence of enterocin B structural gene (entB) was detected in MYE58 strain. MYE58 strain did not exhibit haemolysis and gelatinase activity. In MYE58 strain, the presence of gelE and espfs genes were detected, but agg, ace, efaAfs, ccf, cob, cpd, cat, cylM, cylB, cylA, vanA and vanB genes were not detected. MYE58 was found resistant to streptomycin and tetracycline. The results of this study showed that enterocin B producer E. faecalis MYE58 strain used as starter culture could pose a risk to consumer health. However, purified or semi-purified enterocin B produced by MYE58 strain may have a potential to use for food preservation against Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus.

Unveiling the Probiotic Potential of Streptococcus thermophilus MCC0200: Insights from In Vitro Studies Corroborated with Genome Analysis

“…MCC0200 demonstrated remarkable tolerance to SIF for up to 360 min (Table S2), exhibiting a viable count of 1.1 ± 0.28 × 10 7 CFU/mL with only 1 log reduction in viability, suggesting the strain's robustness to survive in bile stress prevalent in GI tract. Our findings were in congruence with the reported literature, wherein, different studies have reported efficient bile tolerance (up to 1% and in some cases even up to 2%) trait of S. thermophilus [2,49,50].…”

“…A study by Taj et al, 2022 [59], has reported auto-aggregation percentages of 97.8 ± 0.4, 61.2± 1.0, and 53.6 ± 0.6 for different strains of S. thermophilus. Tuncer and Tuncer (2014) [49] reported 49.55 ± Figure 2. Adherence of S. thermophilus MCC0200 to the hydrocarbons: chloroform, ethyl acetate and xylene (the BATH method was employed to measure the hydrophobicity of the cell surface of MCC0222.…”

Screening of Yoghurt Cultures for their Potential Proteolytic, Antioxidant and Probiotic Properties

“…Many research reports on cell auto-aggregation have been documented for the screening of LAB including probiotic bacteria. Tuncer and Tuncer, (2014) have reported that the autoaggregation value of S. thermophilus ST8.01 was recorded as 49.55 ± 6.24% which can be comparable with this study and even better results have been shown by the ST 144 and LB 144. Tamara et al, (2012) have also reported that three strains of L. plantarum possessed the ability to auto-aggregation and coaggregation, which are an important feature in the selection of probiotic bacteria.…”

High performance of amidoxime/amine functionalized polypropylene for uranyl (VI) from aqueous solution

“…[1][2][3] Many types of adsorbents have been developed and tested for the recovery of uranium from seawater and aqueous media. To recover uranium from seawater at the price competitive with those from conventional uranium resources, many methods such as solvent extraction [4][5][6][7] ion exchange [8], flotation [9], biomass collection and adsorption [10][11][12][13][14] have been developed. The studies indicate that poly(acrylamidoximes) are the most promising candidate adsorbents for the recovery of uranium from seawater.…”