Safety evaluation of nuclear polyhedrosis virus replication in pigs

Döller, G; Gröner, Albrecht; Straub, O C

doi:10.1128/aem.45.4.1229-1233.1983

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…Baculoviruses have been studied in the past for their ability to infect mammalian cells for safety considerations with regard to their use as pesticides 1–5. No evidence of replication was observed in several human cell lines tested at low multiplicity of infection (M.O.I.…”

Section: Introductionmentioning

confidence: 99%

Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells

et al. 1999

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Background Recently, several reports have described the ability of recombinant baculoviruses to transduce a variety of mammalian cells. Yet, mechanisms involved in baculovirus entry in those cells remain largely unexplored, particularly at the primary binding step of the virions to the cell membrane. Methods This report focused on the primary virus‐cell interactions that lead to in vitro transduction of human 293 cells using a polyhedrin‐deleted baculovirus harboring a CMV‐driven β‐galactosidase gene (BacLacZ). Results Infection rate monitored for 8 h and transduction rate with a multiplicity of infection of up to 800 were, both, non‐saturable. Temperatures from 37°C to 4°C dramatically impaired BacLacZ but not adenovirus cell attachment. Competitive infections performed with an excess of a non LacZ‐expressing baculovirus hardly competed at a 1/1 ratio. Consistent with an adsorptive binding process onto the cell surface, interactions through electrostatic charges between both viral and cell membranes appeared to be critical for BacLacZ transduction. The addition of polybrene to the cells prior to or during the infection prevented both virus binding and LacZ gene transfer, suggesting the involvement of negatively charged epitopes exposed at the cell surface. The simultaneous presence of the highly charged heparin abrogated BacLacZ binding to the cell surface and subsequent gene transfer. Lastly, direct in vitro binding of BacLacZ to heparin but not BSA columns could be demonstrated after elution of infectious BacLacZ virus in high salt molarity. Conclusion Electrostatic charges play a critical role during the first step in mammalian cell transduction mediated by a recombinant baculovirus. Copyright © 1999 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells

et al. 1999

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“…As a gene transfer vector, the baculovirus possesses inherited advantages, including no apparent signs of cytopathic effects in mammalian cells, large gene insert capacity, and a simple production procedure (Ghosh et al 2002;Kost & Condreay, 2002). Being non-mammalian viruses, unmodified wild-type baculoviruses are incapable of replication and expression of viral proteins in mammalian cells (Doller et al 1983;Hartig et al 1992), thus significantly reducing the chance of pre-existing humoral and cellular immunity in mammals.…”

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confidence: 99%

Neuronal gene transfer by baculovirus‐derived vectors accommodating a neurone‐specific promoter

Yang

Wang

2004

Experimental Physiology

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“…In another study, guinea pigs and mice tested with high doses of polyhedral or free baculovirus rods for intradermal allergenicity, and intranasal and intravenous administration confirmed the biosafety associated with these viruses (Heimpel 1966). NPVs tested for propagation, immune reactions and acute infections in pigs demonstrated a good safety profile (Doller et al 1983). A recent study evaluated the safety of Bombyx mori (another widely known baculovirus)-based vaccine in Rhesus monkeys and reported safety even at high doses (Jin et al 2008).…”

Section: Discussionmentioning

confidence: 92%

“…NPVs tested for propagation, immune reactions and acute infections in pigs demonstrated a good safety profile (Doller et al . ). A recent study evaluated the safety of Bombyx mori (another widely known baculovirus)‐based vaccine in Rhesus monkeys and reported safety even at high doses (Jin et al .…”

Section: Discussionmentioning

confidence: 97%

cDNA microarray assays to evaluate immune responses following intracranial injection of baculoviral vectors in non‐human primates

Balasundaram

Kwang

Shu

2016

Journal of Neurochemistry

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Recombinant insect baculoviral vectors efficiently transduce several types of cells in the brain and can possibly be used in gene therapy for brain disorders. However, together with contaminating insect cell proteins, they trigger immune responses that might damage host brain cells. To substantially reduce unwanted immune responses due to the insect cell impurities, we purified and concentrated baculoviral vectors by combining an ion-exchange membrane chromatography method with high-speed centrifugation and demonstrated reduced immune responses of the vector preparations in the mouse brain. To verify the suitability of using these viral vectors for gene therapy strategies in the brain, we evaluated immune reactions and vector toxicity upon acute administration of baculoviral vectors into the brains of cynomolgus macaques. We demonstrated that the virus inoculation caused no abnormalities to non-human primates but induced host anti-viral responses in the brain. With global cellular gene expression profiling, using cDNA microarray technology, we detected that the affected genes were mainly associated with innate immunity, involving the genes of RIG-1-like receptor signaling pathway as the major interferon production pathway. These findings in non-human primates, which share close physiological and genomic similarities with man, may better mimic the responses to baculoviral transduction in the human brain and should be useful in guiding rational therapeutic applications of baculoviral vectors to treat brain disorders.

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Safety evaluation of nuclear polyhedrosis virus replication in pigs

Cited by 13 publications

References 10 publications

Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells

Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells

Neuronal gene transfer by baculovirus‐derived vectors accommodating a neurone‐specific promoter

cDNA microarray assays to evaluate immune responses following intracranial injection of baculoviral vectors in non‐human primates

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