There are three pathways for triacylglycerol (TAG) biosynthesis: De novo TAG biosynthesis, phosphatidylcholine-derived biosynthesis, and cytosolic TAG biosynthesis.Variability in fatty acid composition is mainly associated with phosphatidylcholinederived TAG pathway. Mobilization of TAG-formed through cytosolic pathway into lipid droplets is yet unknown. There are multiple regulatory checkpoints starting from acetyl-CoA carboxylase to the lipid droplet biogenesis in TAG biosynthesis. Although a primary metabolism, only a few species synthesize oil in seeds for storage, and less than 10 species are commercially exploited. To meet out the growing demand for oil, diversifying into newer sources is the only choice left. The present review highlights the potential strategies targeting species like Azadirachta, Callophyllum, Madhuca, Moringa, Pongamia, Ricinus, and Simarouba, which are not being used for eating but are otherwise high yielding (ranging from 1.5 to 20 tons per hectare) with seeds having a high oil content (40-60%). Additionally, understanding the toxin biosynthesis in Ricinus and Simarouba would be useful in developing toxin-free oil plants. Realization of the importance of cell cultures as "oil factories" is not too far into the future and would soon be a commercially viable option for producing oils in vitro, round the clock.
Highlight• Newer alternative sources like cell cultures and high yielding (nonedible) species with high oil content are commercially viable targets to engineer in producing edible-grade oil using the omics tool.