Safety hazards in bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

Rahmdel, Samane; Hosseinzadeh, Saeid; Shekarforoush, Seyed Shahram; Torriani, Sandra; Gatto, Veronica; Pashangeh, Safoora

doi:10.1016/j.micpath.2018.01.016

Cited by 18 publications

(18 citation statements)

References 51 publications

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“…As mentioned for the phenotypic assay, the prevalence of antibiotic-resistant genes varied with the isolates. Discrepancies in the results by Rahmdel et al [ 38 ] were observed, since these authors displayed that tet M gene was present in only 18% of the Staphylococcus strains from goat and sheep milk, while erm B and mec A genes were present in the 85.7% of them. None of the isolates assayed in our study harbored the mec A gene encoding methicillin resistance.…”

Section: Discussionmentioning

confidence: 81%

“…When results from phenotypic and genetic assays were compared, both concordances, as occurred for vancomycin, gentamicin, tetracycline, ciprofloxacin, and erythromycin, and discrepancies, as for β-lactams, between both types of analysis, were obtained. Other authors [ 19 , 20 , 38 ] also reported discrepancies that were explained by the presence of silent genes, by genetic mutations in the coding or promoter regions of the genes, or because the detection by PCR of a single gene in an antibiotic resistance operon may overlook the absence of the other genes involved in phenotypic expression [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

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Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk

Povéda

Jiménez

Perea

et al. 2020

Animals

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Staphylococci are one of the main microorganisms responsible for intramammary infections in sheep, causing important economic losses for farmers and eventually health problems in humans, especially by the consumption of dairy products made with raw milk containing toxic compounds, such as biogenic amines or antibiotic resistant bacteria. This study aimed to check the presence and safety of staphylococci in bulk tank ewe’s milk from different farms, and to determine the relationship between the presence of these staphylococci and farming practices, by applying nonlinear canonical correlation models (OVERALS). Two-hundred and fifty-nine staphylococci from milk samples from eighteen farms were genotyped and representative isolates of the major clusters were identified as belonging to Staphylococcus (S.) aureus, S. epidermidis, S. arlettae, S. lentus, S. simulans, and S. chromogenes species. Identified isolates were assayed in terms of their safety, by evaluating resistance to antimicrobial drugs and the aminobiogenic capacity, using both phenotypic and genetic assays. Antibiotic resistance phenotypic assay revealed that 82.9% were resistant to some antibiotics, although in the genotypic assay only the genes tetM, ermB, ermC, and grlA were detected. Fifty-three percent were high biogenic amine (BA) producers, being putrescine the most produced amine. A lowered risk of finding antibiotic-resistant and BA-producing staphylococci is related to some farming methods such as enrolling in a breeding program, use of good farming practices, postdipping teat disinfection, hygienic livestock housing, or periodic check of the milking machine.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk

Povéda

Jiménez

Perea

et al. 2020

Animals

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“…Much less has been published regarding the antimicrobial effects of Staphylococcus strains on the amine producers (Mah and Hwang, 2009). In a complementary study (Rahmdel et al, 2018), Staph. epidermidis 4S93 was found to be resistant to methicillin.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

Rahmdel

Shekarforoush

Hosseinzadeh

et al. 2019

Journal of Dairy Science

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Bacteriocins have attracted great attention as potential alternatives to antibiotics and chemical food additives. In the present study, 243 Staphylococcus isolates from milk samples (n = 110) of goat and sheep herds located in Fars province, Iran, were screened for antimicrobial substance production. Twenty-eight isolates showed an antagonistic activity against the indicator strain Micrococcus luteus ATCC 4698. The susceptibility of all antimicrobial substances to proteolytic enzymes allowed us to consider them as bacteriocin-like inhibitory substances (BLIS). The term BLIS is applied to uncharacterized proteinaceous antimicrobials produced by gram-positive bacteria. Based on molecular identification methods, the isolates belonged to the species Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus pseudintermedius, Staphylococcus aureus, and Staphylococcus agnetis. Pulsed-field gel electrophoresis revealed a high level of genotype diversity among the Staph. chromogenes isolates. All of the isolates harbored nukA or bsaA2 genes, suggesting that their BLIS were related to nukacin or Bsa. The antimicrobial compounds from test strains were not effective against gram-negative pathogens, including Salmonella enterica serovar Typhimurium, Escherichia coli O157:H7, and Klebsiella pneumonia as well as the indicator mold Aspergillus fumigatus. All the gram-positive targets, including Bacillus cereus, Listeria monocytogenes, Enterococcus faecalis Ef37 (a tyramine-producer strain), Lactobacillus saerimneri 30a (a histamine-producer strain), and methicillin-resistant Staph. epidermidis, were inhibited by the Staph. chromogenes isolates. Staphylococcus haemolyticus 4S12 was able to inhibit the majority of gram-positive bacteria. Listeria monocytogenes strains were the only indicators sensitive to the antimicrobial agents produced by Staph. agnetis 4S97B. The other Staphylococcus strains were ineffective on all the organisms tested. Based on their inhibitory capacities, the BLIS produced by the Staph. chromogenes isolates seem to be interesting candidates for developing novel antimicrobial agents.

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“…The exfoliative toxin gene etd was observed in one isolate and c-hemolysin in two (Mahato et al 2017). The presence of genes encoding for enterotoxins seb and sec, and for toxic shock syndrome toxin tst1, in an isolate of S. agnetis obtained from milk has also been described (Rahmdel et al 2018).…”

Section: Staphylococcus Agnetismentioning

confidence: 84%

“…Biofilm production, b-hemolysis and cytotoxic activity to Vero cells have been described in S. hyicus isolated from beef meat in Egypt (Osman et al 2016). As previously mentioned, genes encoding enterotoxins seb and sec, and for toxic shock syndrome toxin tst1, in an isolate of S. agnetis obtained from small ruminant milk were observed (Rahmdel et al 2018). In the same study, two strains of S. pseudintermedius, one of them carrying genes sea, seb, sec, see and tst1 and the other, sec and tst1 were found.…”

Section: Human Health Implications Of These Virulence Factorsmentioning

confidence: 86%

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

González-Martín

Corbera

Suárez‐Bonnet

et al. 2020

Veterinary Quarterly

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Coagulase-positive Staphylococci (CoPS) can exist as commensals in humans, companion and food-producing animals, but can cause severe or even lethal diseases. Exchange of these bacteria between humans and animals has been described. Special attention has been focused on Methicillin-Resistant Staphylococcus aureus, but other CoPS can also represent an important threat. In addition to significant antimicrobial resistance, these bacteria may carry a plethora of virulence factors-molecules that allow bacteria to establish on or within a host and increase their ability to cause disease. These virulence factors have been widely described in S. aureus but information about other species of CoPS is scarce. The aim of this paper is to review the recent literature about the virulence factors of non-aureus CoPS of animal origin. Their possible effects on human health are also described. The role and prevalence of different virulence factors including leukocidins, hemolysins, adhesins, enterotoxins, exfoliative and toxic shock syndrome toxins as well as superantigen-like proteins are addressed. The effect of these virulence factors on human health is also described. The possibility of misdiagnosis of species of CoPS has been demonstrated in human clinical samples. Prevalence of zoonotic infections could be higher than thought and medical laboratories should be aware of these other staphylococcal species. In keeping with the 'One Health' approach to animal and human disease, medical professionals, veterinarians and health workers should be aware of the risks derived from exposure to these bacteria in people in close contact with animals, including pet owners, farmers and veterinarians themselves.

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Safety hazards in bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

Cited by 18 publications

References 51 publications

Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk

Farming Practices Influence Antibiotic Resistance and Biogenic Amine Capacity of Staphylococci from Bulk Tank Ewe’s Milk

Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

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