Safety of Allogeneic Canine Adipose Tissue-Derived Mesenchymal Stem Cell Intraspinal Transplantation in Dogs with Chronic Spinal Cord Injury

Abstract

This is a pilot clinical study primarily designed to assess the feasibility and safety of X-ray-guided percutaneous intraspinal injection of allogeneic canine adipose tissue-derived mesenchymal stem cells in dogs with chronic spinal cord injury. Six dogs with chronic paraplegia (≥six months) were intraparenchymally injected with allogeneic cells in the site of lesion. Cells were obtained from subcutaneous adipose tissue of a healthy dog, cultured to passage 3, labeled with 99mTechnetium, and transplanted into the lesion by percutaneous X-ray-guided injection. Digital X-ray efficiently guided cell injection as 99mTechnetium-labeled cells remained in the injection site for at least 24 hours after transplantation. No adverse effects or complications (infection, neuropathic pain, or worsening of neurological function) were observed during the 16-week follow-up period after transplantation. Three animals improved locomotion as assessed by the Olby scale. One animal walked without support, but no changes in deep pain perception were observed. We conclude that X-ray-guided percutaneous intraspinal transplantation of allogeneic cells in dogs with chronic spinal cord injury is feasible and safe. The efficacy of the treatment will be assessed in a new study involving a larger number of animals.

Avaliação sensitiva, motora e cistométrica de cães com lesão medular crônica, submetidos ao transplante de células-tronco mesenquimais derivadas de tecido adiposo

“…três linhagens diferentes (Dominici et al 2006 Também de acordo com os critérios estabelecidos para definição de células-tronco mesenquimais, as células-tronco de cães utilizadas neste estudo, demonstraram o potencial de diferenciação em adipócitos, osteoblastos e condrócitos. Outros estudos utilizando CTDAs também relatam a diferenciação nestas três linhagens celulares (Neupane et al 2008, Escalhão et al 2017.…”

Multidose intramuscular allogeneic adipose stem cells decrease the severity of canine atopic dermatitis: A pilot study

“…On the other hand, the use of mesenchymal stem cell (MSC) therapy, especially bone marrow-derived MSCs (BM-MSCs) and adipose stem cells (ASCs), has mainly been supported by the particular traits of these cells, including cell renewal, differentiation, and production/release of a vast array of growth factors, cytokines, and other types of mediators of cell function [24,25]. Furthermore, the use of allogeneic MSC has shown to be effective and safe in canine diseases [26]. In addition, adult MSCs exhibit attractive immunomodulatory properties in degenerative diseases in animals [27].…”

Can Paraplegia by Disruption of the Spinal Cord Tissue Be Reversed? The Signs of a New Perspective

“…However, although the results are encouraging, the mechanism under the clinical improvement is still unknown, even though its paracrine, immunomodulatory, and anti‐inflammatory effects are established (Teng et al, ; Myckatyn et al, ; Keirstead et al, ; Kang et al, ; Ryu et al, ; Park et al, ; Oda et al, ; Penha et al, ; Kim et al, ; Oh and Jeon, ; Filippi et al, ). Besides that, information regarding MSCs approach to treat subacute and chronic spinal cord injuries is still poor (Lee et al, ; Escalhão et al, ). Here we associated two therapeutic approaches, physiotherapy and MSCs infusion, to treat dogs suffering from subacute and chronic spinal cord injury naturally caused.…”