Safety of atazanavir/ritonavir with tenofovir disoproxil fumarate in HIV-infected adolescents

Abstract

Background: APOBEC3G potently controls HIV replication by introducing G-to-A hypermutation. Genetic variants of APOBEC3G, 186H/R is associated with HIV/AIDS disease progression in adults. However, the details of its effect in children remain unclear.Methods & Materials: Rapid progressors (RPs) was defined as children who had AIDS-related symptoms (PCP, HIV encephalopathy) before their second birthday. Long-term non-progressors (LTNPs) were defined as children, aged>8 years, maintaining CD4%≥25%, and without cART. RPs and LTNPs were of 630 perinatally infected children who were enrolled in the multicenter studies of Thailand and Cambodia.APOBEC3G genotypes were determined by PCR-restriction fragment length polymorphism method using genomic DNA samples. APOBEC3G-mediated hypermutations were analyzed by sequencing of the vif/vpu genes from proviral DNAs.Results: A total of 14 RPs and 24 LTNPs were enrolled. 50% of RP and 67% of LTNP were female (p=0.3). Median ages at sample collection were 11.