There are no reports of herbicide tolerance on seedling seashore paspalum (Paspalum vaginatum Sw.). The objective of this study was to determine which herbicides cause the least amount of injury to seashore paspalum seedlings and allow for maximum establishment. Field studies were conducted in 2007 and 2008 to assess the tolerance of ‘Sea Spray’ seashore paspalum to 23 herbicides. Herbicide treatments were applied two weeks after emergence of seedlings and compared to an untreated control. MSMA, imazaquin, fluazifop, triclopyr, siduron, and ethofumesate injured seedlings and reduced establishment. Clopyralid, halosulfuron, metsulfuron, quinclorac, carfentrazone, and salt water provided the least injury to seashore paspalum seedlings while allowing maximum establishment.