Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease

Zhao, Yi; Xi, Gao

doi:10.1080/13880209.2018.1501705

Cited by 12 publications

(5 citation statements)

References 14 publications

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Section: Resultsmentioning

confidence: 99%

“…RA-induced differentiated SH-SY5Y cells exhibit the dopaminergic neuron phenotype and express tyrosine hydrogenase (TH) and dopamine transporter (DAT), both of which are involved in the production of dopamine. 24 Because we found that nAg10 inhibited RA-induced neuronal differentiation, we hypothesized that nAg10 may also inhibit the development of the dopaminergic neuron phenotype; we therefore evaluated the effect of nAg10 on dopamine production and on the expression of TH and DAT in RA-treated SH-SY5Y cells. The concentration of dopamine in the culture supernatant was significantly decreased in cells treated with RA + nAg10 (0.1− 2.5 μg/mL) compared with that in the supernatant from cells treated with RA only, and the decrease was nAg10concentration-dependent (Figure 3A).…”

Section: ■ Introductionmentioning

confidence: 99%

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Silver Nanoparticles Suppress Retinoic Acid-Induced Neuronal Differentiation in Human-Derived Neuroblastoma SH-SY5Y Cells

Yamaguchi

Isaka

Sakahashi

et al. 2022

ACS Appl. Nano Mater.

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Nanoparticles are being used in an increasing number of applications in a wide range of fields; however, these particles can migrate to the brain, raising concerns over their potential as risk factors of neurodevelopment disorders. Here, we examined the effects of silver nanoparticles with a diameter of 10 nm (nAg10) on neural differentiation in human-derived neuroblastoma SH-SY5Y cells. SH-SY5Y cells treated with retinoic acid undergo neuronal differentiation characterized by increased expression of brain-derived neurotrophic factor (BDNF); however, co-treatment of these cells with retinoic acid (RA) and nAg10 significantly mitigated this RA-induced BDNF expression. RA-induced neurite outgrowth and extracellular secretion of dopamine were significantly suppressed by nAg10 in a concentration-dependent manner. Reactive oxygen species (ROS) production was enhanced in cells co-treated with RA and nAg10, and the expression level of BDNF was restored by co-treatment with nAg10 and an ROS inhibitor (N-acetylcysteine). nAg10-induced mitochondrial ROS production and decreased mitochondrial fusion-related gene expression. In summary, nAg10 suppressed retinoic acid-induced neuronal differentiation in SH-SY5Y cells via the oxidative stress pathway, induced mitochondrial ROS production, and decreased mitochondrial fusion-related gene expression.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Silver Nanoparticles Suppress Retinoic Acid-Induced Neuronal Differentiation in Human-Derived Neuroblastoma SH-SY5Y Cells

Yamaguchi

Isaka

Sakahashi

et al. 2022

ACS Appl. Nano Mater.

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“…The above results indicated that Safranal alleviated the symptoms of motor disorders in Parkinson’s mouse and it may be used to treat Parkinson’s disease. Moreover, there have been two previous studies on Safranal and Parkinson’s disease, one of which was Yi Zhao et al demonstrated that Safranal promoted the production of functional DA cells and alleviated PD through in vitro and in vivo rat models [ 39 ], the other was P-K Pan et al, indicated that Safranal protected against rotenone-induced neurotoxicity associated with Nrf2 signaling pathway in vitro model of PD [ 40 ]. Surprisingly, their research was consistent with our research.…”

Section: Discussionmentioning

confidence: 99%

Safranal exerts a neuroprotective effect on Parkinson’s disease with suppression of NLRP3 inflammation activation

Yang,

Wei,

Sun

et al. 2024

Mol Biol Rep

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Background Parkinson’s disease (PD) is a common central nervous system neurodegenerative disease. Neuroinflammation is one of the significant neuropathological hallmarks. As a traditional Chinese medicine, Safranal exerts anti-inflammatory effects in various diseases, however, whether it plays a similar effect on PD is still unclear. The study was to investigate the effects and mechanism of Safranal on PD. Methods The PD mouse model was established by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine MPTP firstly. Next, the degree of muscle stiffness, neuromuscular function, motor retardation and motor coordination ability were examined by observing and testing mouse movement behavior. Immunofluorescence staining was used to observe the expression of tyrosine hydroxylase (TH). The dopamine (DA) content of the striatum was detected by High-performance liquid chromatography (HPLC). The expression of TH and NLRP3 inflammasome-related markers NLRP3, IL-1β, and Capase-1 were detected by Real-time Polymerase Chain Reaction (qRT-PCR) and western blotting (WB) respectively. Results Through behavioral testing, Parkinson’s mouse showed a higher muscle stiffness and neuromuscular tension, a more motor retardation and activity disorders, together with a worse motor coordination compared with sham group. Simultaneously, DA content and TH expression in the striatum were decreased. However, after using Safranal treatment, the above pathological symptoms of Parkinson’s mouse all improved compared with Safranal untreated group, the DA content and TH expression were also increased to varying degrees. Surprisingly, it observed a suppression of NLRP3 inflammation in the striatum of Parkinson’s mouse. Conclusions Safranal played a neuroprotective effect on the Parkinson’s disease and its mechanism was related to the inhibition of NLRP3 inflammasome activation.

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“…This neuroprotection was observed due to anti-apoptotic effect, down regulation of inflammatory reactions, and edema weakening in spinal cord of rats. Its nerve growth stimulation was also verified in dopamine cells and this indicated use of Safranal as a novel drug for the treatment of Parkinson's disease (PD) ( Zhao and Xi, 2018 ). This neurodegenerative disease is characterized by slow progress of dopamine (DA) neuron degeneration.…”

Section: Safranal In Neurodegerative Diseasesmentioning

confidence: 94%

The role of Safranal and saffron stigma extracts in oxidative stress, diseases and photoaging: A systematic review

Nanda

Madan

2021

Heliyon

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Reactive oxygen species (ROS) are produced as a result of various environmental factors and cellular metabolism reactions creating oxidative stress. The reversible oxidative modification on proteins such as cysteine oxidation may be useful and can play positive role. ROS generated offer some benefits such as cellular signalling and tissue repair when present in low concentration. However, most of the times, these reactive species cause detrimental effects to cell components which leads to various pathological conditions which causes or aggravates diseases due to oxidative stress. The degenerative diseases due to oxidative stress are diabetes, cardiovascular diseases, epilepsy, cancer and aging. Antioxidants are the compounds which scavenge these free radicals and hence neutralize their effects. Research has enabled the use of natural antioxidants as therapeutic agent in the treatment of diseases. Safranal is one such natural agent which is a major volatile component of saffron. Saffron, Red gold is the most expensive spice found in limited region of the planet and is also reported to be used in traditional systems of medicine. Chemically, safranal is a monoterpene aldehyde possessing a sweet fragrance. While exploring for the photoprotective properties of safranal, we learnt about the immense antioxidant potential of safranal. Investigation by various research groups established safranal as an anti-inflammatory, antidepressant, anxiolytic, antiasthamatic, antihypertensive, anticonvulsant, anticancer and antitussive and antigenotoxic agent. It has brought researchers over the world to explore the antioxidant benefits of saffron for human health. In the present paper, potential of safranal and its related molecules as radical scavenger in combating oxidative stress, diseased conditions is collated and the underlying mechanisms have been explained. Various cell lines and animal models used for study of Safranal have been discussed.

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Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson’s disease

Cited by 12 publications

References 14 publications

Silver Nanoparticles Suppress Retinoic Acid-Induced Neuronal Differentiation in Human-Derived Neuroblastoma SH-SY5Y Cells

Silver Nanoparticles Suppress Retinoic Acid-Induced Neuronal Differentiation in Human-Derived Neuroblastoma SH-SY5Y Cells

Safranal exerts a neuroprotective effect on Parkinson’s disease with suppression of NLRP3 inflammation activation

The role of Safranal and saffron stigma extracts in oxidative stress, diseases and photoaging: A systematic review

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