Safranin O Staining Using a Microwave Oven

Kahveci, Zeynep; Minbay, Fatma; Cavusoglu, L

doi:10.3109/10520290009085130

Cited by 33 publications

(24 citation statements)

References 23 publications

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“…After 5 days of fixation, the knee joints were decalcified using a decalcifying solution (24.4% formic acid and 0.5 N sodium hydroxide) for 5 days, with exchange of the mixed decalcifying solution once per day over the 5 days. Next, median joints were longitudinally trimmed and embedded in paraffin, sectioned (3-4 µm) and stained with Safranin O for cartilage visualization as described previously (21)(22)(23). Histological profiles of the knee joints were evaluated using Mankin scoring (24,25), and the thickness of articular cartilage was compared with that of the intact control.…”

Section: Measurement Of Cartilage Glycosaminoglycan (Gag)mentioning

confidence: 99%

Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9 weight ratio, on rats with surgery-induced osteoarthritis

Choi¹,

Shin²,

Kim³

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. In the present study, the beneficial and synergistic effects of Polycalcium, a mixture of Polycan and calcium (Ca) lactate-gluconate in a 1:9 weight ratio, on a rat model of osteoarthritis (OA) were explored. Polycalcium (50, 100 and 200 mg/kg) was administered orally once per day for 28 days from 1 week after the OA-modeling surgery. Diclofenac sodium (2 mg/kg) was administered as a reference drug. Following the OA surgery, increases in the maximum extension angles, edematous changes in knee and capsule thickness, reductions in chondrocyte proliferation and cartilage glycosaminoglycan (GAG) levels, as well as changes in cartilage degeneration were observed. However, these OA-related symptoms were inhibited after 28 days of continuous oral treatment with Polycalcium. Anti-OA effects, including the induction of chondrocyte proliferation, were detected in the Polycalcium-treated rats and were more favorable compared with those in rats treated with Polycan or Ca lactate-gluconate alone (100 mg). Therefore, a mixture of Polycan and Ca lactate-gluconate was demonstrated to have beneficial synergistic effects on OA.

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Section: Measurement Of Cartilage Glycosaminoglycan (Gag)mentioning

confidence: 99%

Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9 weight ratio, on rats with surgery-induced osteoarthritis

Choi¹,

Shin²,

Kim³

et al. 2015

Experimental and Therapeutic Medicine

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“…Samples 5-8 μm in thickness were cut transversely and longitudinally, and several 31 conventional stains were used in the seminal vesicles to identify the TC staining affinity. These included hematoxylin and eosin [Harris, 1898], Mallory trichrome [Mallory, 1936], resorcin-fuchsin nuclear red after Weigert [1898], van Gieson's method [1989], Gabe's technique [1976], the Safranin O method [Kahveci et al, 2000], and Marsland's method [Marseland et al, 1945].…”

Section: Samples For Light Microscopymentioning

confidence: 99%

Effect of Melatonin on Telocytes in the Seminal Vesicle of the Soay Ram: An Immunohistochemical, Ultrastructural and Morphometrical Study

Abd‐Elhafeez

Mokhtar

Hassan³

2016

Cells Tissues Organs

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Telocytes (TCs) are a special type of interstitial cell with characteristic cellular processes that are described in many organs. The current study aimed to investigate TCs in seminal vesicles of the Soay ram responding to melatonin treatment during the nonbreeding season by conventional immunohistochemical stains, and to detect the ultrastructural and morphometrical changes of TCs. TCs in the control group showed a broad range of staining affinity and also reacted positively to CD117/c-kit, CD34, desmin, S-100 protein, and progesterone and estrogen receptors alpha, while after melatonin treatment a strong reaction against these 6 antibodies was recorded. Electron microscopically, TCs in the control group were characterized by a small cell body with distinct long cytoplasmic extensions called telopodes (Tps). Tps had alternation of the thin segment (podomers) and dilated segments (podoms), in which the latter accommodate mitochondria, rough endoplasmic reticulum and caveolae. TCs and their Tps were interconnected by homo- and heterocellular junctions and form a wide network to communicate between different cell types. Tps showed close contact with immune cells, progenitor stem cells, smooth muscle cells and other interstitial cells. Melatonin caused a significant increase in the number of TCs, length of Tps, and number and diameter of secretory vesicles. Also, the melatonin-treated group showed exaggerated secretory activity in the form of a massive release of secretory vesicles from Tps. Moreover, Tps showed an increase in their contact with blood and lymphatic capillaries, nerve endings and Schwann cells. In addition, the shedding of secretory structures (exosomes, ectosomes, and multivesicular bodies) was greater from Tps, which were involved in paracrine signaling in the melatonin-treated group. The length and ramifications of Tps together with the intercellular junctions and the releasing of shed vesicles or exosomes assumed an essential role of TCs in intercellular signaling and coordination. On the basis of their distribution and morphology, we investigated whether the different locations of TCs could be associated with different roles.

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“…This staining method was modified by Kahveci et al (2000) and Tran et al (2000) and was used for enhancing growth bands in Torpedo marmorata (Duman and Başusta, 2013).…”

Section: Safranin Omentioning

confidence: 99%

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Başusta¹,

Demirhan²,

Çiçek³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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In this study, six chondrichthyan species (Raja clavata, Raja miraletus, Raja asterias, Torpedo marmorata, Rhinobatos rhinobatos and Gymnura altavela) were captured for age determination in Iskenderun Bay (North eastern Mediterranean Sea) between May 2010 and January 2012. Age was determined from vertebral sectioned, using four staining techniques, namely Crystal Violet, Silver Nitrate, Safranin O and Alcian Blue. The results from these four techniques were compared to each other, with respect to visibility success on growth rings. Of all four staining techniques, Silver Nitrate staining provided the best optical resolution for growth rings of R. clavata, R. miraletus and R. rhinobatos. For, R. asterias, T. marmorata and G. altavela, however, it provided a lower optical resolution. In fact, four staining technique was also efficient for R. clavata. The most efficient staining techniques were Safranin O in T. marmorata and Alcian Blue in R. rhinobatos. The order of average readability scores for staining techniques was as follows: Silver Nitrate = Safranin O > Crystal Violet = Alcian Blue. A standardized staining methodology that can be used for all species studied, however, has not been achieved.Keywords: Age determination, Cartilaginous Fish, Crystal Violet, Silver Nitrate, Alcian Blue, Safranin O. Keywords: Bazı Kıkırdaklı Balık Türlerinde Yaş Tayininde Kullanılan Boyama Yöntemlerinin Karşılaştırılması ÖzetBu çalışmada altı kıkırdaklı balık türü (Raja clavata, Raja miraletus, Raja asterias, Torpedo marmorata, Rhinobatos rhinobatos and Gymnura altavela) yaş tayini amacıyla Mayıs 2010 -Ocak 2012 tarihleri arasında İskenderun Körfezi'nde yakalanmıştır. Yaş tayini Kristal Viyole, Gümüş Nitrat, Safranin O ve Alsian Mavisi boyama yöntemleri kullanılarak omur kesitlerinden yapılmıştır. Bu dört teknik büyüme halkalarındaki görünürlülük başarısı açısından birbirleriyle karşılaştırılmıştır. Bu dört boyama tekniğinden gümüş nitrat, R. clavata, R. miraletus ve R. rhinobatos türlerinin büyüme halkaları için en iyi görüntü çözünürlüğünü sağlamıştır. R. asterias, T. marmorata ve G. altavela türleri için ise daha düşük görüntü çözünürlüğü vermiştir. Aslında, R. clavata için dört boyama tekniği de etkili olmuştur. R. rhinobatos omurlarındaki büyüme halkalarını belirgenleştirmek için en etkili boyama tekniği Alsian Mavisi ve T. marmorata için ise Safranin O olmuştur. Boyama teknikleri için ortalama okunabilirlik puanları sırası: Gümüş Nitrat = Safranin O > Kristal Viyole = Alsian Mavisi. Bununla birlikte, türlerin hepsinde kullanılabilecek standart bir boyama tekniği bulunamamıştır.

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Safranin O Staining Using a Microwave Oven

Cited by 33 publications

References 23 publications

Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9 weight ratio, on rats with surgery-induced osteoarthritis

Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9 weight ratio, on rats with surgery-induced osteoarthritis

Effect of Melatonin on Telocytes in the Seminal Vesicle of the Soay Ram: An Immunohistochemical, Ultrastructural and Morphometrical Study

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