Sage in vitro cultures: a promising tool for the production of bioactive terpenes and phenolic substances

Abstract

Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to their content of terpenes and polyphenols. The increasing demand for sage products combined with environmental, ecological and climatic limitations on the production of sage metabolites from field-grown plants have led to extensive investigations into biotechnological approaches for the production of Salvia phytochemicals. The purpose of this review is to evaluate recent progress in investigations of sage in vitro systems as tools for producing important terpenoids and polyphenols and in development of methods for manipulating regulatory processes to enhance secondary metabolite production in such systems.

Anti-apoptotic Effect of Taxodione on Serum/Glucose Deprivation-Induced PC12 Cells Death

“…Inhibition of butyryl-and acetyl-cholinesterase is also reported for this non-toxic, European herbal species (Kennedy and Scholey 2006). Salvia species display a broad spectrum of pharmacological properties (Marchev et al 2014). Salvia chorassanica is an endemic species of the salvia in Iran (Emami and Aghazari 2011).…”

“…Terpenes and polyphenols content are thought to be responsible for the bioactivity of sage extracts (Marchev et al 2014). Multiple diterpenoids including taxodione have been extracted from the roots of S. chorassanica (Tayarani-Najaran et al 2013).…”

Rhodiola rosea L.: from golden root to green cell factories

“…Since salidroside and CAGs are dominantly accumulated in the roots of the plant, therefore hairy roots should be a reliable alternative for their biosynthesis. Hairy roots are genetically and metabolically stable and are able to produce secondary metabolites similar to the intact plants: hence they can be used as a sustainable platform for in vitro biosynthesis of secondary metabolites (Marchev et al 2014;Georgiev 2014). The single report in the available literature for hairy root induction belongs to R. rosea is from Tasheva and Kosturkova (2012b).…”

“…In vitro cultures possess numerous advantages, including biosynthesis of biosafe metabolites according to the good manufacturing practices (GMP) and independency of the environmental factors. Also 6 opportunities for facilitating the down-stream processes resulting in increased yields of the target metabolites through different biotechnological approaches are available (Steingroewer et al 2013;Marchev et al 2014;Grech-Baran et al 2015).…”

Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants

“…As compared to field cultivation, in vitro techniques offer the possibility of continuous production of large amounts of chemically uniform biomass independently of wild resources and environmental factors (Murthy et al 2014). As far as essential oils are concerned, in vitro cultivation also gives the possibility to obtain novel compounds, previously not reported in intact plants (Gounaris 2010;Gonçalves and Romano 2013;Marchev et al 2014). Since the accumulation of volatile constituents was shown to be positively correlated with cell differentiation, most experiments aimed at establishing plant in vitro systems for essential oil production were conducted with the use of organ cultures.…”