The aim of this study is to determine the levels of healthy lifestyle behaviors and well-being of individuals who exercise and to examine the effect of healthy lifestyle behaviors on basic psychological needs and well-being. A total of 297 individuals who do sports, whom 195 of woman and 102 of men, are included in the study. Basic Psychological Needs in Exercise, Healthy Lifestyle Behavior and Psychological Well-Being scales were used to determine the basic psychological needs, well-being during exercise and healthy lifestyles of individuals participating in physical activity. To evaluate the data were applied ANOVA, pearson correlation analysis and multiple regression analysis. The results showed that there was a low positive correlation between the subscales of basic psychological needs in exercise and healthy lifestyle behaviors and well-being levels of exercising individuals. In addition, it was found that yoga practitioners had higher mean scores of well-being and health responsibility, nutrition, physical activity, stress management, spiritual development, subscales than fitness and pilates.