Saikosaponin a attenuates hyperlipidemic pancreatitis in rats via the PPAR‑γ/NF‑κB signaling pathway

Abstract

The therapeutic effect of saikosaponin a (SSa) on hyperlipidemic pancreatitis (HP) is not completely understood. The aim of the present study was to investigate the therapeutic effect and the underlying mechanism of SSa using a rat model of HP. Following successful establishment of the HP rat model, different doses of SSa (low dose group, 10 mg/kg or high dose group, 20 mg/kg) were administrated. Histopathological examination, the wet/dry (W/D) ratio and myeloperoxidase (MPO) activity of the pancreatic tissues were assessed. The lipid, amylase (AMY), lipase and proinflammatory cytokine profiles in serum, as well as the expression of peroxisome proliferator-activated receptor (PPAR)-γ and the NF-κB signaling pathway-related proteins in pancreatic tissues were evaluated. The results showed that SSa effectively attenuated pancreatic pathological injury and reduced both the W/D ratio and MPO activity compared to the HP model rats. SSa also improved lipid metabolism by significantly decreasing the serum levels of total cholesterol and triglycerides (P<0.05). Following the administration of SSa, the activity of AMY and lipase, as well as the levels of the proinflammatory cytokines tumor necrosis factor-α, interleukin (IL)-1β and IL-6 were reduced, particularly in the high dosage group (P<0.05). Furthermore, SSa activated PPAR-γ expression and suppressed the NF-κB signaling pathway in pancreatic tissues. The present study suggested that SSa attenuated HP in rats by increasing lipid metabolism and inhibiting the release of proinflammatory cytokines via the NF-κB inflammatory pathway. The results from the present study indicated that SSa might be a promising therapeutic agent for the treatment of HP.

Ephedra alata extracts exerts anti-obesity, anti-hyperglycemia, anti-antipyretic and analgesic effects

“…Accordingly, recent research seems to have granted special interest for the search of effective natural glucosidase and lipase inhibitors (Hamden et al, 2017). Previous research studies have shown that medicinal plants exhibited pronounced inhibitory effects towards the activities of a-amylase, lipase and inflammation (Kang et al, 2015;Ghasemian et al, 2016;Von Dentz, et al, 2020;Joshi et al, 2018;Feng et al, 2020;Mohamed et al, 2020;Tiss et al, 2020). In fact, Plants are a source of bioactive secondary metabolites such as glycosides, saponins, flavonoids, steroids, tannins, alkaloids and terpenes, which act as strong antioxidant to protect body from oxidative damage (Upreti et al, 2019).…”

HIF-1α-PPARγ-mTORC1 signalling pathway mediated autophagy induces inflammatory response of pancreatic cells in rats with hyperlipidemic acute pancreatitis

“…The peroxisome proliferators-activated receptor γ(PPARγ) is mainly expressed in liver and adipose tissues and it plays a role in promoting the differentiation and maturation of adipocytes and regulating the activation of signaling pathways responsible for lipid metabolism and the expression of related genes [21]. It is believed that hyperlipidemia can exacerbate the in ammatory response and pathological injury of AP, the mechanisms of which may be related to the down regulation of PPARγ mRNA expression [ 22,23]. For this reason, treatment with PPARγ agonist can relieve the in ammatory response of pancreatic tissues in HLAP.…”

Phytochemicals with protective effects against acute pancreatitis: a review of recent literature

“…In addition, it significantly decreased the levels of pancreatic enzymes (MPO, amylase and lipase) and reduced proinflammatory cytokines (TNF‑α, IL-1β and IL-6) release by suppressing the NF-κB signalling pathway and promoting the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) (Feng et al. 2019 ). Taken together, SSa exerted therapeutic effects on hyperlipidaemic pancreatitis.…”