Saikosaponin-d ameliorates dextran sulfate sodium-induced colitis by suppressing NF-κB activation and modulating the gut microbiota in mice

Li, Puze; Wu, Minna; Xiong, Wancheng; Li, Jinsong; An, Yunying; Ren, Jie; Ying-guang, Xie; Xue, Hongfei; Yan, Dong; Li, Min; Zhong, Genshen

doi:10.1016/j.intimp.2020.106288

Cited by 61 publications

(39 citation statements)

References 61 publications

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“…Red indicates positive correlations, and blue indicates negative correlations. Significant differences were indicated as *P < 0.05. study (Li et al, 2020), treatment with DSS resulted in the decrease of mRNA and protein expression of Claudin-1, Occludin, and ZO-1. Algal oil administration improved the expression of Claudin-1, Occludin, and ZO-1 in colonic tissues.…”

Section: Discussionmentioning

confidence: 88%

Algal Oil Rich in n-3 PUFA Alleviates DSS-Induced Colitis via Regulation of Gut Microbiota and Restoration of Intestinal Barrier

Tang

Huang

et al. 2020

Front. Microbiol.

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Algal oil is rich in docosahexaenoic acid (DHA) and has various health benefits against human metabolic disorders and disease. This study aimed to investigate the effects of DHA algal oil on colonic inflammation and intestinal microbiota in dextran sulfate sodium (DSS)-induced colitis mice model. Male C57BL/6 mice was induced colitis by 2.5% DSS and followed by 2 weeks of treatment with algal oil (250 or 500 mg/kg/day). The colonic inflammation was assessed by colon macroscopic damage scores, and the degree of neutrophil infiltration was evaluated by measuring tissue-associated myeloperoxidase (MPO) activity in colonic mucosa. Tight junction proteins in the colonic tissue were measured by real-time PCR and western blot. Moreover, the intestinal microbiota and shot chain fatty acids (SCFAs) were estimated by bioinformatic analysis and GC, respectively. Colonic damage due to DSS treatment was significantly ameliorated by algal oil supplementation. In addition, algal oil significantly inhibited the increases of malondialdehyde (MDA) content, MPO activity, pro-inflammatory cytokines level and tight junction proteins expression in DSS-treated mice. Furthermore, supplementation of algal oil modulated the intestinal microbiota structure in DSS induced colitis mice by increasing the proportion of the unidentified_S24_7 and decreasing the relative abundance of unidentified_Ruminococcaceae, Clostridium and Roseburia. On the analysis of SCFAs, the caecal content of acetic acid, propionic acid, isobutyric acid, buturic, and the total SCFAs showed a significant increase in algal oil-administered mice. Together, these results suggested that algal oil rich in DHA inhibited the progress of DSS-induced colitis in mice by modulating the intestinal microbiota and metabolites and repairing the intestinal barrier, which may be applied in the development of therapeutics for intestinal inflammation.

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Section: Discussionmentioning

confidence: 88%

Algal Oil Rich in n-3 PUFA Alleviates DSS-Induced Colitis via Regulation of Gut Microbiota and Restoration of Intestinal Barrier

Tang

Huang

et al. 2020

Front. Microbiol.

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“…The Ruminiclostridium 6 abundance was positively correlated with the cytokines, DAI, and the pathological score. Thus, the increasing abundance of Ruminiclostridium 6 could be an indicator of colitis [ 36 ]. As expected, the present findings show that NaB treatment can improve the gut microbial dysbiosis by decreasing the abundance of Ruminiclostridium 6.…”

Section: Discussionmentioning

confidence: 99%

Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis

Dou

Gao

Yan

et al. 2020

Animals

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Inflammatory bowel disease (IBD) develops as a result of complicated interactions between genetic susceptibility, excessive innate immunity, and environmental factors, which are mainly related to the gut microbiota. The present study aimed to elucidate the protective effects and underlying mechanisms of a short-chain fatty acid salt, sodium butyrate, on colonic inflammation induced by dextran sulfate sodium (DSS) in mice. Pretreatment with sodium butyrate attenuated colitis, as demonstrated by the decreased disease activity index (DAI), colon length shortening, spleen tumidness, and histopathology scores, while maintaining intestinal barrier integrity, as observed by H&E staining and electron microscopy. 16S rRNA sequence analysis revealed that sodium butyrate caused a remarkable alteration of the gut microbiota. Bacteroides, Lachnospiraceae, the Lachnospiraceae NK4A136 group, and Ruminiclostridium 6 presented dramatic differences after sodium butyrate supplementation. This work verifies that sodium butyrate can improve mouse colitis via microbe–host interactions by regulating the microbial community. Taken together, the findings demonstrate that sodium butyrate shows great potential as a probiotic agent for ameliorating colitis.

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“…Actually, the anti-inflammatory and anti-infectious activities of saikosaponin D have been reported previously. Saikosaponin D can relieve ventilator-induced lung injury by decreasing oxidative stress and apoptosis and inhibiting the expression of proinflammatory cytokines in rats ( Li et al, 2020 ), indicating that saikosaponin D could be served as a potential candidate for the treatment of inflammatory-related and lung diseases.…”

Section: Resultsmentioning

confidence: 99%

“…The World Health Organization announces the COVID-19 pandemic on March 11, 2020 ( CDC COVID-19 Response Team et al, 2020 ). Cluster evidence of infected family members and medical staff confirms the existence of person-to-person transmission through droplets, contact and poisonous gas, causing an infection of>8000,000 people worldwide, with > 435,000 deaths and the WHO has declared the epidemic to be a global public health emergency ( Li et al, 2020 , Cui et al, 2018 ). Although researchers are racing against time to develop vaccines and test anti-viral drugs in clinical trials, there is no appropriate drug or effective vaccine against the current coronavirus until now ( Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 98%

Systematic analyses on the potential immune and anti-inflammatory mechanisms of Shufeng Jiedu Capsule against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)-caused pneumonia

Tao

Zhang

Friedemann

et al. 2020

Journal of Functional Foods

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Saikosaponin-d ameliorates dextran sulfate sodium-induced colitis by suppressing NF-κB activation and modulating the gut microbiota in mice

Cited by 61 publications

References 61 publications

Algal Oil Rich in n-3 PUFA Alleviates DSS-Induced Colitis via Regulation of Gut Microbiota and Restoration of Intestinal Barrier

Algal Oil Rich in n-3 PUFA Alleviates DSS-Induced Colitis via Regulation of Gut Microbiota and Restoration of Intestinal Barrier

Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis

Systematic analyses on the potential immune and anti-inflammatory mechanisms of Shufeng Jiedu Capsule against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)-caused pneumonia

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