Sailuotong Prevents Hydrogen Peroxide (H2O2)-Induced Injury in EA.hy926 Cells

Seto, Sai Wang; Chang, Dennis Hsu-Tung; Ko, Waiman; Zhou, Xian; Kiat, Hosen; Bensoussan, Alan; Lee, Simon Ming‐Yuen; Hoi, Maggie Pui Man; Steiner, Genevieve Z.; Liu, Jianxun

doi:10.3390/ijms18010095

Cited by 27 publications

(34 citation statements)

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“…Generally, the ratio of Bax to Bcl-2 is used to evaluate the degree of apoptosis. When the ratio increases, and apoptosis will be aggravated [ 57 ]. The results revealed that H 2 O 2 increased the ratio of Bax to Bcl-2, resulting in exacerbating apoptosis of IPEC-J2, and Que reduced the ratio of Bax to Bcl-2 in IPEC-J2, showing a better inhibitory effect on apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells

Chen

Yuan

et al. 2018

Molecules

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Weanling stress and toxicosis, which are harmful to the health of pigs’ intestines, are associated with oxidative stress. Quercetin (Que) is a polyphenolic compound that shows good anti-cancer, anti-inflammation and anti-oxidation effects. This study aimed to elaborate whether or not Que promotes IPEC-J2 (intestinal porcine enterocyte cells) proliferation and protects IPEC-J2 from oxidative damage. Thus, we examined the effects of Que on proliferation and H2O2-induced apoptosis in IPEC-J2. The results showed that Que increased IPEC-J2 viabililty, propelled cells from G1 phase into S phase and down-regulated gene levels of P27 and P21, respectively. Besides, H2O2-induced cell damage was alleviated by Que after different exposure times, and Que depressed apoptosis rate, reactive oxygen species (ROS) level and percentage of G1 phase cells and elevated the percentage of cells in G2 phase and S phase and mitochondrial membrane potential (Δψm) after IPEC-J2 exposure to H2O2. Meanwhile, Que reduced the value of Bax/Bcl-2 in H2O2 exposed cells. In low-degree oxidative damage cells, lipid peroxidation product malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were increased. In turn, Que could reverse the change of MDA content and SOD activity in low-degree damage cells. Nevertheless, catalase (CAT) activity was not changed in IPEC-J2 incubated with Que under low-degree damage conditions. Interestingly, relative expressive levels of the proteins claudin-1 and occludin were not altered under low-degree damage conditions, but Que could improve claudin-1 and occludin levels, slightly. This research indicates that Que can be greatly beneficial for intestinal porcine enterocyte cell proliferation and it protects intestinal porcine enterocyte cells from oxidation-induced apoptosis, and could be used as a potential feed additive for porcine intestinal health against pathogenic factor-induced oxidative damages and apoptosis.

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Section: Discussionmentioning

confidence: 99%

Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells

Chen

Yuan

et al. 2018

Molecules

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“…In a chronic cerebral hypoperfusion model induced by bilateral common carotid artery ligation in rats, eight-week treatment of SLT significantly suppressed hypoperfusion-induced cognitive impairments, and this change is associated with reduction of activity of cholinesterase and increased acetylcholine (ACh) levels and superoxide dismutase (SOD) activity the brain tissue [ 8 ]. Our previous work also showed that SLT protected cultured human vascular endothelial cells against hydrogen peroxide-induce injury via increase in SOD activity [ 12 ]. The potential beneficial effects of SLT for VaD were demonstrated in two separate phase II randomized, double-blinded, placebo-controlled clinical studies.…”

Section: Introductionmentioning

confidence: 99%

Endothelium‐Independent Vasodilatory Effect of Sailuotong (SLT) on Rat Isolated Tail Artery

Yeon

Seto

Chan

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Sailuotong (SLT) is a standardized three-herb formulation consisting of extracts of Panax ginseng, Ginkgo biloba, and Crocus sativus for the treatment of vascular dementia (VaD). Although SLT has been shown to increase cerebral blood flow, the direct effects of SLT on vascular reactivity have not been explored. This study aims to examine the vasodilatory effects of SLT and the underlying mechanisms in rat isolated tail artery. Methods. Male (250–300 g) Wistar Kyoto (WKY) rat tail artery was isolated for isometric tension measurement. The effects of SLT on the influx of calcium through the cell membrane calcium channels were determined in Ca2+-free solution experiments. Results. SLT (0.1–5,000 μg/ml) caused a concentration-dependent relaxation in rat isolated tail artery precontracted by phenylephrine. In the contraction experiments, SLT (500, 1,000, and 5,000 μg/mL) significantly inhibited phenylephrine (0.001 to 10 μM)- and KCl (10–80 mM)-induced contraction, in a concentration-dependent manner. In Ca2+-free solution, SLT (500, 1,000, and 5,000 μg/mL) markedly suppressed Ca2+-induced (0.001–3 mM) vasoconstriction in a concentration-dependent manner in both phenylephrine (10 μM) or KCl (80 mM) stimulated tail arteries. L-type calcium channel blocker nifedipine (10 μM) inhibited PE-induced contraction. Furthermore, SLT significantly reduced phenylephrine-induced transient vasoconstriction in the rat isolated tail artery. Conclusion. SLT induces relaxation of rat isolated tail artery through endothelium-independent mechanisms. The SLT-induced vasodilatation appeared to be jointly meditated by blockages of extracellular Ca2+ influx via receptor-gated and voltage-gated Ca2+ channels and inhibition of the release of Ca2+ from the sarcoplasmic reticulum.

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“…Vascular protective effects of single herbal extracts and/or their isolated bioactive component on AAA have been demonstrated in a number of animal studies ( Table 1 ). However, direct evidence to support the use of complex herbal formulations for AAA is limited even though a number of complex Chinese herbal formulations, such as Si Miao Yong An (SMYA) ( 51 ), Sai Lou Tong (SLT) ( 16 ), Tong Xin Luo (TXL) capsule ( 52 ) and Huang Lian Jie Du Tang ( 53 ), have been shown to have vascular protective effects. In a head-to-head comparison study between TXL capsule (consisting of Radix ginseng , Buthus martensii , Hirudo medicinalis , Eupolyphaga seu steleophaga , Scolopendra subspinipes , Periostracum cicadae , Paeoniae rubra , Semen ziziphi spinosae , Lignum dalbergiae odoriferae , Lignum santali albi , and Borneolum syntheticum ) and simvastatin, both TXL and simvastatin at high dose showed to enhance plaque stability with similar lipid-lowering, anti-inflammatory and anti-oxidative effects in a rabbit model of atherosclerosis.…”

Section: Chm As Potential Drug Therapy For Aaamentioning

confidence: 99%

“…Many CHMs have been shown to have remarkable anti-oxidative properties ( 107 , 108 ). For instance, Sailuotong (SLT), a standardised three-herb formula combining specific dosages of Panax ginseng (Ren Shen), Ginkgo biloba (Yin Xin Ye), and Crocus sativus (Zhuang Hong Hua), has been shown to protect endothelial cell from ROS-induced apoptosis via enhancement of superoxide dismutase (SOD) activity and suppression of caspase-3 activation ( 16 ). EGb 761, a standardized extract of Ginkgo biloba is a well-known anti-oxidant ( 109 , 110 ).…”

Section: Chm As Potential Drug Therapy For Aaamentioning

confidence: 99%

Chinese Herbal Medicine as a Potential Treatment of Abdominal Aortic Aneurysm

Seto

Chang

Kiat

et al. 2018

Front. Cardiovasc. Med.

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Abdominal aortic aneurysm (AAA) is an irreversible condition where the abdominal aorta is dilated leading to potentially fatal consequence of aortic rupture. Multiple mechanisms are involved in the development and progression of AAA, including chronic inflammation, oxidative stress, vascular smooth muscle (VSMC) apoptosis, immune cell infiltration and extracellular matrix (ECM) degradation. Currently surgical therapies, including minimally invasive endovascular aneurysm repair (EVAR), are the only viable interventions for AAAs. However, these treatments are not appropriate for the majority of AAAs, which measure <50 mm. Substantial effort has been invested to identify and develop pharmaceutical treatments such as statins and doxycycline for this potentially lethal condition but these interventions failed to offer a cure or to retard the progression of AAA. Chinese herbal medicine (CHM) has been used for the management of cardiovascular diseases for thousands of years in China and other Asian countries. The unique multi-component and multi-target property of CHMs makes it a potentially ideal therapy for multifactorial diseases such as AAA. In this review, we review the current scientific evidence to support the use of CHMs for the treatment of AAA. Mechanisms of action underlying the effects of CHMs on AAA are also discussed.

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Sailuotong Prevents Hydrogen Peroxide (H2O2)-Induced Injury in EA.hy926 Cells

Cited by 27 publications

References 50 publications

Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells

Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells

Endothelium‐Independent Vasodilatory Effect of Sailuotong (SLT) on Rat Isolated Tail Artery

Chinese Herbal Medicine as a Potential Treatment of Abdominal Aortic Aneurysm

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