Sairei-to Ameliorates Rat Peritoneal Fibrosis Partly through Suppression of Oxidative Stress

Abstract

Background: Sairei-to is a herbal prescription originating from traditional Chinese medicine. We conducted an experimental study on rat peritoneal fibrosis to clarify the suppressive mechanisms of sairei-to. Methods: Wistar rats were intraperitoneally injected with chlorhexidine gluconate (CG) every day. Peritoneal specimens were collected after 28 days of CG injection and oral administration of sairei-to. Macrophage infiltration, extracellular matrix accumulation, and angiogenesis were evaluated by immunostaining for ED-1, fibronectin, and CD-31, respectively. To observe oxidative stress in the tissue, 4-hydroxy-2-noneal (HNE) accumulation and plasma levels of superoxide dismutase (SOD) activity were detected. As a candidate of antioxidative components in sairei-to, plasma levels of baicalin were determined by high-performance liquid chromatography. Results: Compared with the disease control group, serum total protein levels were significantly recovered in the sairei-to treatment group. Thickness of the submesothelial compact zone, trichrome-stained area, ED-1-positive cells, fibronectin-staining area, and HNE accumulation were suppressed in the treatment group. Concurrently, decreased plasma levels of SOD activity were recovered by sairei-to treatment. Increased CD-31-positive vessel number and area were also suppressed in the sairei-to group. Baicalin was detected in the plasma samples of the sairei-to group at 0.29 ± 0.11 µg/ml (mean±SEM). Conclusion: These results suggest that sairei-to ameliorates peritoneal fibrosis, partly through suppressing oxidative stress and macrophage infiltration.

Involvement of Leptin in the Progression of Experimentally Induced Peritoneal Fibrosis in Mice

“…Although the precise mechanism of these findings is unknown, leptin and leptin receptor expressions have been suggested to be regulated by various mediators such as inflammatory cytokines and oxidative stress. Given that the pathophysiological mechanism was reported to be involved in inflammation or oxidative stress in a CG-induced peritoneal fibrosis model [10, 35], these factors might upregulate the expression of not only leptin but also the leptin receptor in our study.…”

Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114)

“…( 29 ) Saireito thus far ameliorated mesangioproliferative glomerulonephritis and peritoneal fibrosis in rats. ( 30 , 31 ) We recently showed in the rat CDH model that antenatal Saireito increased the lung volume and alveolarization and respiratory functions while decreasing endothelin-1 expression and vascular remodelling, thus lowering the incidence of CDH. ( 32 ) Here, we focused on iron metabolism and oxidative stress in nitrofen-induced rat CDH and its prevention by Saireito.…”

Antenatal Saireito (TJ-114) Can Improve Pulmonary Hypoplasia and Pulmonary Vascular Remodeling in Nitrofen-Induced Congenital Diaphragmatic Hernia

“…Saireito is commonly used to clinically treat pregnant women with edema and is covered by public health insurance in Japan. Saireito contains several ingredients that increase the synthesis and secretion of adrenocorticotropic hormone (Tozawa et al, 1998), increase superoxide dismutase activity (Ono et al, 2005), decrease phosphodiesterase activity (Saegusa et al, 2003), reduce inflammatory responses (Leung et al, 2005), and inhibit angiogenesis (Kitamoto et al, 2011). Interestingly, the therapeutic effects produced by Saireito cannot be obtained using the individual ingredients, or mixtures thereof, and therefore, the effect of Saireito depends on its specific composition of herbal ingredients (Zhang et al, 2009).…”

“…In addition, saikosaponin, which is derived from the Bupleurum root, exhibits a variety of pharmacological and immunomodulatory activities, including antiinflammatory responses (Leung et al, 2005). Baicalin, which is derived from the root of Scutellaria baicalensis, has antioxidant and antiangiogenic effects (Kitamoto et al, 2011). Thus, the ingredients of Saireito have a wide variety of therapeutic effects.…”