2005
DOI: 10.2144/05393st03
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SAIVGeM: Spreadsheet Analysis of Immunoglobulin V H Gene Mutations

Abstract: The analysis of mutations in immunoglobulin heavy chain variable (IGHV) region genes is a tedious process when performed by hand on multiple sequences. This report describes a set of linked Microsoft Excel files that perform several common analyses on large numbers of IGHV sequences. The spreadsheet analysis of immunoglobulin VH gene mutations (SAIVGeM) package determines the distribution of mutations among each nucleotide, the nature of the mutation at both the nucleotide and amino acid level, the frequency o… Show more

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Cited by 3 publications
(1 citation statement)
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“…IGHV1-72*01 members were selected using IMGT V-QUEST and taken forward in analysis. Mutation frequencies and amino acid changes were identified using the SAIVGEM package ( Messmer, 2005 ). MiSeq analysis was performed by first merging paired-end reads using PANDAseq ( Masella et al, 2012 ).…”
Section: Methodsmentioning
confidence: 99%
“…IGHV1-72*01 members were selected using IMGT V-QUEST and taken forward in analysis. Mutation frequencies and amino acid changes were identified using the SAIVGEM package ( Messmer, 2005 ). MiSeq analysis was performed by first merging paired-end reads using PANDAseq ( Masella et al, 2012 ).…”
Section: Methodsmentioning
confidence: 99%