SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways

Yao, Chunyan; Fu, Wenliang; Gan, Xiaosong; Xing, Weiwei; Xia, Wenrong; Zou, Minji; Liu, Qing; Wang, Yuan‐Yuan; Zhang, Chao; Xu, Donggang

doi:10.7150/ijbs.27459

Cited by 5 publications

(3 citation statements)

References 46 publications

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“…Cell migration assay for RAW264.7 cells was performed as described previously with minor changes 33 . Briefly, 2.5 × 10 5 RAW264.7 cells in 1 mL normal medium were added to ThinCerts cell culture inserts (Greiner Bio-One, Kremsmünster, Austria, 657630) in 6 well plates while 2.5 mL of 48 h conditioned medium from MLEC or ISOS-1 cells were added to the lower wells.…”

Section: Methodsmentioning

confidence: 99%

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

Gulay

Aoshima

Maekawa

et al. 2022

Sci Rep

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Hemangiosarcoma (HSA) is a malignant tumor derived from endothelial cells. Tumor-associated macrophages are one of the major components of tumor microenvironment and crucial for cancer development. The presence and function of macrophages in HSA have not been studied because there is no syngeneic model for HSA. In this study, we evaluated two mouse HSA cell lines and one immortalized mouse endothelial cell line for their usefulness as syngeneic models for canine HSA. Our results showed that the ISOS-1 cell line developed tumors with similar morphology to canine HSA. ISOS-1 cells highly expressed KDM2B and had similar KDM2B target expression patterns with canine HSA. Moreover, we determined that in both ISOS-1 and canine HSA tumors, macrophages were present as a major constituent of the tumor microenvironment. These macrophages were positive for CD204, an M2 macrophage marker, and express PD-L1, an immune checkpoint molecule. Canine HSA with macrophages expressing PD-L1 had a smaller number of T-cells in tumor tissues than tumors with PD-L1 negative macrophages. ISOS-1-conditioned medium could induce M2 polarization and PD-L1 expression in RAW264.7 mouse macrophage cell line and mouse peritoneal macrophages. These results show that ISOS-1 can be used as a syngenic model for canine HSA and suggest that macrophages play an important role in immune evasion in HSA. Using the syngeneic mouse model for canine HSA, we can further study the role of immune cells in the pathology of HSA.

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Section: Methodsmentioning

confidence: 99%

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

Gulay

Aoshima

Maekawa

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Migration assay for RAW264.7 cells was performed as described previously with minor changes 33 . Briefly, 2.5 × 10 5 RAW264.7 cells in 1 mL normal medium were added to ThinCerts cell culture inserts (Greiner Bio-One, Kremsmünster, Austria) in 6 well plates while 2.5 mL of 48 hours conditioned medium from MLEC or ISOS-1 cells were added to the lower wells.…”

Section: Methodsmentioning

confidence: 99%

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

Gulay

Aoshima

Maekawa

et al. 2021

Preprint

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Hemangiosarcoma (HSA) is a malignant tumor derived from endothelial cells. Tumor-associated macrophages are one of the major components of tumor microenvironment and crucial for cancer development. The presence and function of macrophages in HSA have not been studied because there is no syngeneic model for HSA. In this study, we evaluated two mouse HSA cell lines and one immortalized mouse endothelial cells for their usefulness as syngeneic models for canine HSA. Our results show that the ISOS-1 cell line develops tumors with similar morphology to canine HSA. ISOS-1 cells highly express KDM2B and have similar KDM2B target expression patterns with canine HSA. Moreover, we determine that in both ISOS-1 and canine HSA tumors, macrophages are present as a major constituent of the tumor microenvironment. These macrophages are positive for CD204, an M2 macrophage marker, and express PD-L1. ISOS-1-conditioned medium can induce M2 polarization and PD-L1 expression in RAW264.7 mouse macrophage cell line. These results show that ISOS-1 can be used as a syngenic model for canine HSA and suggest that macrophages play an important role in immune evasion in HSA. Using the syngeneic mouse model for canine HSA, we can further study the role of immune cells in the pathology of HSA.

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“…In immune system, macrophages belonging to the important cells. Proliferation and differentiation ability of macrophage plays important role in the innate immune system and a prerequisite for immune and inflammatory responses [ 1 ]. In hematopoietic system, macrophages are one of the most plastic cells.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-455-3p regulates proliferation and osteoclast differentiation of RAW264.7 cells by targeting PTEN

Zhang

Wang

Huang

et al. 2022

BMC Musculoskelet Disord

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Background Macrophages are one of the important cells in immune system. In this article, we aim to explore the regulatory role of miR-455-3p on proliferation and osteoblast differentiation of RAW264.7 cells. Methods Expression levels of genes and proteins in cells were tested via qRT-PCR and western blot. The targeted correlation between miR-455-3p and PTEN was identified by luciferase analysis. MTT assay and flow cytometry were applied to detect the proliferation and apoptosis of cells. Osteoclastogenesis was completed by stimulating RAW 264.7 cells with RANKL. Tartrate-resistant acid phosphatase (TRAP) activity in different groups of cells were assessed. Results Firstly, we determined that up-regulation of miR-455-3p promoted the proliferation and inhibited apoptosis of RAW 264.7 cells. MiR-455-3p deficiency played opposite effect in RAW 264.7 cells. Additionally, osteoclastogenesis-related factors (TRAP, CTSK and NFATc1) expression levels were remarkably up-regulated in miR-455-3p-mimic group of RAW264.7 cells treated with RANKL, but decreased in inhibitor group. Luciferase assay proved that miR-455-3p targeted PTEN. We took a further step and found overexpression of PTEN significantly inhibited the increased proliferation and osteoblast differentiation of RAW264.7 cells induced by miR-455-3p. Conclusions Our findings supported basic to explore the molecular mechanism of proliferation and osteoblast differentiation of RAW264.7 cells.

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SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-κB Pathways

Cited by 5 publications

References 46 publications

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

MicroRNA-455-3p regulates proliferation and osteoclast differentiation of RAW264.7 cells by targeting PTEN

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