Salacia mulbarica leaf extract mediated synthesis of silver nanoparticles for antibacterial and ct‐DNA damage via releasing of reactive oxygen species

Abstract

In this examination, we researched the advantages of DNA fragmentation and metallic nanoparticles well-appointed with biomolecules. A novel interpretation of DNA damage by Silver Nano-Clusters (AgNCs) which were developed by the utilization of green synthesis method was demonstrated. The green synthesis of AgNCs was accomplished by utilizing the leaf extract of Salacia mulbarica (SM). The preparation of SM-AgNCs was developed by estimating surface plasmon resonance peak around 449 nm by using a UV-Visible spectrophotometer. The effect of phytochemicals in SM leaf extract on the development of stable SM-AgNCs was confirmed by FTIR spectroscopy. The size of the fabricated SM-AgNCs was estimated by dynamic light scattering and zeta-sizer analysis and the morphology of the SM-AgNCs was examined by transmission electron microscopy. The presence of clusters of Ag particles in the prepared SM-AgNCs was recognized by energy dispersion X-ray analysis. The results show that saponins, phytosterols, and phenolic compounds present in plant extract may play a great part in developing the SM-AgNCs in their specialized particles. The succeeded SM-AgNCs shows incredible anti-bacterial action towards Escherichia coli and Bacillus subtilis. In-light of the antibacterial study, these SM-AgNCs were analyzed with calf thymus-DNA and found significant damage to the strand of thymus-DNA.

Synthesis of Novel Tamarind Gum-co-poly(acrylamidoglycolic acid)-Based pH Responsive Semi-IPN Hydrogels and Their Ag Nanocomposites for Controlled Release of Chemotherapeutics and Inactivation of Multi-Drug-Resistant Bacteria

“…Essentially, the reduction of silver ions to silver nanoparticles is proven by a color change from light yellow to black at ambient temperature and a reaction time of 180 min, as shown in Figure 3 . Significant absorption peaks were detected at 428–436 nm as a result of a quantum mechanical process known as surface plasmon resonance, which leads in the creation of silver nanoparticles within polymer networks with the help of polymer chains [ 31 , 32 ].…”

“…The DOX-loaded TMGA hydrogel ( Figure 4 D) showed characteristic DOX peaks at two theta values: 17.52°, 19.53° and 25.29°, showing that DOX is physically present, chemically stable and molecularly distributed within the hydrogel network. The XRD pattern of the TMGA-Ag hydrogel ( Figure 4 E) showed typical peaks at 38.46°, 44.41°, 63.12° and 76.46° for planes (111), (200), (220) and (311), respectively [ 31 , 32 ]. These findings suggest that the silver nanoparticles found in the hydrogel network are face-centered cubic crystals in nature.…”

Synthesis of Novel Tamarind Gum-co-Poly(Acrylamidoglycolic Acid) based pH Responsive semi-IPN Hydrogels and their Ag Nanocomposites for Controlled Release of Chemotherapeutics and Inactivation of Multi Drug Resistance Bacteria

“…The results show that TMGbased hydrogels can be successfully synthesized using simple free radical polymerization, DOX can be incorporated in TMGA hydrogels, and silver nanoparticles can be produced in an environmentally safe manner. Figure 2 detected at 428-436 nm as a result of a quantum mechanical process known as surface plasmon resonance, which leads in the creation of silver nanoparticles within polymer networks with the help of polymer chains [32,33]. 200), (220), and (311), respectively [32,33].…”

“…Figure 2 detected at 428-436 nm as a result of a quantum mechanical process known as surface plasmon resonance, which leads in the creation of silver nanoparticles within polymer networks with the help of polymer chains [32,33]. 200), (220), and (311), respectively [32,33]. These findings suggest that the silver nanoparticles found in the hydrogel network are facecentered cubic crystals in nature.…”

Salacia spp.: recent insights on biotechnological interventions and future perspectives

“…Recently, Nagesh et al ( 2022 ) synthesized silver nanomaterials from the roots of S. chinensis , which is able to modulate biological properties such as antibacterial, antifungal, and antiproliferative effects. The leaf extract–derived silver nanoparticles from S. mulbarica proved to be a better antibacterial agent against E. coli and B. subtilis and also possess the capability of ct-DNA damage via releasing of reactive oxygen species (Espenti et al 2020 ). In recent times, environmentally benign silver bio-nanomaterials have been synthesized from the root extracts of S. oblonga and served as potent antioxidant, antibacterial, and anti-diabetic agents (Dugganaboyana et al 2023 ).…”