Sale-based estimation of pharmaceutical concentrations and associated environmental risk in the Japanese wastewater system

He, Kai; Borthwick, Alistair G.L.; Lin, Yingchao; Li, Yuening; Fu, Jie; Wong, Yong Jie; Liu, Wen

doi:10.1016/j.envint.2020.105690

Cited by 41 publications

(21 citation statements)

References 89 publications

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“…They showed that the PEC was close to and sometimes overlapped the MEC, but differences between PEC and MEC were significant for some PhCs. Conversely, Ong et al [43] and He et al [44] demonstrated the usefulness of the predictive model in Melbourne (Australia) and Japan, respectively, whereas Neves et al [45] successfully slightly modified the model for 10 of the most used PhCs in Belo Horizonte (Brazil). The modification was based on refinements of the parameters (i.e., human consumption excretion/removal and dilution factors) of the formula, allowing a better representation of the city of study.…”

Section: Introductionmentioning

confidence: 99%

Predicted and Measured Concentration of Pharmaceuticals in Surface Water of Areas with Increasing Anthropic Pressure: A Case Study in the Coastal Area of Central Italy

Cardini

Pellegrino

Ercoli

2021

Water

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This study investigated the occurrence of 12 pharmaceuticals (PhCs) in surface water in Central Italy, aiming to improve the estimation of the predicted environmental concentration (PEC) by normalizing the loads to the number of inhabitants of the drainage system in rural, periurban, and urban areas. We performed two sampling campaigns assessing the concentration of PhCs (measured environmental concentration (MEC)) in surface water and in effluent from a wastewater treatment plant. The reliability of PEC calculated by the refined formula was assessed and compared to the ratio obtained by the unrefined formula. MECs of diclofenac, estradiol, estrone, ibuprofen, metformin, naproxen, sulfamethoxazole, atenolol, carbamazepine, and dehydro-erythromycin were significantly higher in urban than in periurban and rural areas, and increases were 12-, 3600-, 256-, 33-, 18-, 120-, 10-, 5-, 2-, and 1-fold, respectively. Refinement of PEC improved estimation of PhC concentrations for all areas, especially for the urban one. The environmental risk was predicted as low for atenolol, carbamazepine, erythromycin, metformin, and naproxen; low/medium for diclofenac and ibuprofen; and high for clarithromycin, estradiol, estrone, and sulfamethoxazole. Overall, the highest risk was posed by PhCs in effluent, while a progressively decreasing risk was estimated for urban, periurban, and rural areas.

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Section: Introductionmentioning

confidence: 99%

Predicted and Measured Concentration of Pharmaceuticals in Surface Water of Areas with Increasing Anthropic Pressure: A Case Study in the Coastal Area of Central Italy

Cardini

Pellegrino

Ercoli

2021

Water

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“…The principle of wastewater-based surveillance was first applied for environmental surveillance of polio in the late 1980s in Israel (Hovi et al, 2012 ), although the term WBE was theorized in 2001 by Daughton and Jones-Lepp ( 2001 ). Since then, the WBE approach has been used extensively in monitoring cocaine consumption (Mao et al, 2020 ; Tang et al, 2020 ; Zuccato et al, 2005 ), pharmaceutical consumption (Baz-Lomba et al, 2016 ; He et al, 2020 ), viral infections (Hou et al, 2020 ) and infections with antibiotic-resistant bacteria (Castrignanò et al, 2020 ; Hutinel et al, 2019 ). During the current COVID-19 pandemic, WBE has been used extensively in several countries to monitor SARS-CoV-2 infections (Ahmed et al, 2020 ; Kumar et al, 2020 ; La Rosa et al, 2020 ; Lodder & de Roda Husman, 2020 ; Medema et al, 2020 ; Randazzo et al, 2020 ; Rimoldi et al, 2020 ; Wu et al, 2020a , b ; Wurtzer et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

RT-LAMP: A Cheaper, Simpler and Faster Alternative for the Detection of SARS-CoV-2 in Wastewater

et al. 2021

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Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has the potential to become a cheaper and faster option for monitoring COVID-19 infections through wastewater-based epidemiology. However, its application in COVID-19 surveillance has been limited to clinical testing only. We present in this paper two optimized RT-LAMP protocols based on colour change and fluorescence detection and application of these protocols for wastewater monitoring from four wastewater treatment plants over 4 weeks. The optimized RT-LAMP protocols have a limit of detection of 10 copies/25 µl reaction with positive amplification within 35 minutes. Over the 4 weeks of monitoring, the colorimetric protocol detected a prevalence of 12.5%, when 1 µl of extracted RNA with 92.7(± 28.2) ng/µl concentration was analysed. When the RNA template was increased by fivefold, the prevalence increased to 44%. The fluorescent RT-LAMP had a prevalence of 31% and 47% for starting templates of 92.7(± 28.2) ng/µl and 480(± 134.5) ng/µl of the extracted RNA, respectively. All samples were positive for SARS-CoV-2 when analysed with droplet digital PCR, with viral loads ranging from 18.1 to 195.6 gc/ml of wastewater. The RT-ddPCR, therefore, confirms the presence of the viral RNA in the wastewater samples, albeit at low concentrations. Additionally, the RT-LAMP protocols positively detected SARS-CoV-2 in wastewater samples with copies as low as 20.7 gc/ml. The results obtained in our study show the potential application of RT-LAMP for the detection of SARS-CoV-2 in wastewater, which could provide a cheaper and faster alternative to RT-qPCR or RT-ddPCR for wastewater-based epidemiological monitoring of COVID-19 and other viral infections.

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“…Due to these adverse effects, it is important to estimate the consumption and emission levels of antibiotics in a specific region or country. In the literature, a number of studies did attempt to estimate the consumption of antibiotics by different models [12][13][14]. However, these studies all had fundamental limitations due to lack of basic information, sales, and/or census data.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Seven Antibiotics and Five of Their Metabolites in Municipal Wastewater and Evaluation of Their Stability under Laboratory Conditions

Han

Huang

et al. 2021

IJERPH

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The selection and spread of antibiotic resistance poses risks to public health by reducing the therapeutic potential of antibiotics against human pathogens. Wastewater-based epidemiology (WBE) is potentially the most reliable approach to estimate antibiotics use. Previous WBE studies used parent antibiotics as biomarkers, which may lead to overestimation since parent antibiotics may be directly disposed of. Using metabolites as biomarkers can avoid this drawback. This study developed a simultaneous solid-phase extraction coupled with ultra-high-performance liquid chromatography tandem mass spectrometry method for analyzing 12 antibiotics and human metabolites in wastewater to help assess health risk. Optimum conditions were achieved using a PEP cartridge at pH 3.0. The extraction efficiencies were 73.3~95.4% in influent and 72.0~102.7% in effluent for most of the target analytes. Method detection limit ranged from 0.1 to 1.5 ng/L for influent wastewater and 0.03 to 0.7 ng/L for effluent wastewater. A stability experiment showed that sulfonamide parents and their metabolites were stable at 4 °C, −20 °C and −80 °C, while macrolides metabolites were more stable than their corresponding parents at 4 °C and −20 °C. Finally, the method was applied to measure these analytes in wastewater samples collected from three Beijing WWTPs and to derive apparent removal rates. All metabolites were detected in wastewater samples with concentrations ranging from 1.2 to 772.2 ng/L in influent, from <MDL to 235.6 ng/L in effluent. The apparent removal rates of five metabolites were above 72.6%. These results set a solid foundation for applying WBE to evaluate antibiotics use and its public health effects.

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Sale-based estimation of pharmaceutical concentrations and associated environmental risk in the Japanese wastewater system

Cited by 41 publications

References 89 publications

Predicted and Measured Concentration of Pharmaceuticals in Surface Water of Areas with Increasing Anthropic Pressure: A Case Study in the Coastal Area of Central Italy

Predicted and Measured Concentration of Pharmaceuticals in Surface Water of Areas with Increasing Anthropic Pressure: A Case Study in the Coastal Area of Central Italy

RT-LAMP: A Cheaper, Simpler and Faster Alternative for the Detection of SARS-CoV-2 in Wastewater

Simultaneous Determination of Seven Antibiotics and Five of Their Metabolites in Municipal Wastewater and Evaluation of Their Stability under Laboratory Conditions

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