Salibaculum halophilum gen. nov., sp. nov. and Salibaculum griseiflavum sp. nov., in the family Rhodobacteraceae

Zhu, Ke-Lei; Wang, Xiaoqun; Shang, Dan-Dan; Du, Zong‐Jun

doi:10.1099/ijsem.0.004808

Cited by 17 publications

(4 citation statements)

References 41 publications

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“…Expression of the sulphur-oxidizing (SOX) system was tested by using a medium (0.5 % peptone, 0.1 % yeast extract and 0.03 % sodium pyruvate), prepared with artificial seawater (MgCl 2 0.23 %, MgSO 4 0.32 %, CaCl 2 0.12 %, KCl 0.07 % and NaS 2 O 4 0.5 %, all w/v). S 2 O 3 2− and SO 4 2− were detected by FeCl 3 and BaCl 2 solutions at 0 and 7 days [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and genotypic characterization of Marinobacterium weihaiense sp. nov. and Marinobacterium marinum sp. nov., isolated from marine sediment, and genomic properties of the genus Marinobacterium

Liu,

Zhu,

et al. 2024

Microbial Genomics

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In this study, two novel bacterial strains were isolated from coastal sediment of Weihai, China. The two strains were Gram-stain-negative and facultatively aerobic, designated 3-1745T and A346T. Based on phenotypic, genetic and phylogenetic properties, strains 3-1745T and A346T represent two novel species of the genus Marinobacterium . The results of genome analysis revealed many central carbohydrate metabolism pathways such as gluconeogenesis, pyruvate oxidation, tricyclic acid cycle, pentose phosphate pathway and PRPP biosynthesis in the genus Marinobacterium . The ability of strains 3-1745T and A346T to utilize volatile fatty acids was experimentally confirmed. Polyhydroxyalkanoate synthases (PhaA, PhaB and PhaC) for the synthesis of polyhydroxyalkanoates were prevalent in the genus Marinobacterium . Multiple BGCs (biosynthetic gene clusters) including betalactone, ectoine, ranthipeptide, redox-cofactor, RiPPs (ribosomally synthesized post-translationally modified peptides) and T3PKS (polyketide synthases) in the genome of the genus Marinobacterium were found. Additional genome analyses suggested that the genus Marinobacterium contained diverse potential mechanisms of salt tolerance and mainly utilized oligosaccharides. This is the first report on broad genomic analyses of the genus Marinobacterium with the description of two novel species and potential ecological and biotechnological implications.

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Section: Methodsmentioning

confidence: 99%

Phenotypic and genotypic characterization of Marinobacterium weihaiense sp. nov. and Marinobacterium marinum sp. nov., isolated from marine sediment, and genomic properties of the genus Marinobacterium

Liu,

Zhu,

et al. 2024

Microbial Genomics

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“…Genomic DNA of the two strains was extracted and purified using a Bacterial Genomic DNA Mini Kit (Takara Bio) according to the manufacturer's recommendations. The 16S rRNA gene of both strains was amplified by PCR using universal primers 27F and 1492R [7]. On the basis of pairwise comparisons of the 16S rRNA gene sequences using the EzBioCloud (www.ezbiocloud.net) [8] and NCBI [National Centre for Biotechnology Information (nih.gov)] data libraries, F47161 T and F64183 T had high similarity in the 16S rRNA gene sequences with A. sinensis JCM 19789 T (97.5 % and 98.4 %, respectively) and A. viscosa JCM 18497 T (97.5 % and 97.4 %, respectively).…”

Section: S Rrna Gene Phylogenymentioning

confidence: 99%

Aequorivita vitellina sp. nov. and Aequorivita xiaoshiensis sp. nov., isolated from marine sediment

Wang

Zhang

Wang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, strictly aerobic, chemoheterotrophic, short-rod-shaped and non-motile strains, forming yellow colonies and designated F47161T and F64183T, were isolated from marine sediment of Xiaoshi Island, Wei Hai, PR China. Strain F47161T grew at 15–37 °C (optimally at 30 °C) and pH 6.0–9.0 (optimally at pH 7.5) and in the presence of 1–9 % (w/v) NaCl (optimally at 3 %). Strain F64183T grew at 10–37 °C (optimally at 30 °C) and pH 6.0–8.5 (optimally at pH 7.0) and in the presence of 1–8 % (w/v) NaCl (optimally at 3 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that F47161T and F64183T were related to members of the genus Aequorivita . The strains shared 97.4 % 16S rRNA gene sequence similarity to each other. F47161T and F64183T shared highest 16S rRNA gene sequence similarity to Aequorivita sinensis JCM 19789T, and the values were 97.5 % and 98.4 %, respectively. The predominant cellular fatty acids of both F64183T and F47161T were iso-C15 : 0 and iso-C17 : 0 3-OH, but the predominant fatty acids of F47161T also included anteiso-C15 : 0. The sole respiratory quinone of F47161T and F64183T was menaquinone 6 (MK-6), consistent with that observed for all related strains. Between F47161T and F64183T, the average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH) values were 75.8 % and 20.5 %, respectively, and between the novel isolates (F47161T and F64183T) and A. sinensis JCM 19789T they were 76.0 % and 94.2 % and 20.6 % and 57.1 %, respectively. The genomic DNA G+C contents of F47161T and F64183T was 37.3 % and 34.5 %, respectively. The polar lipid profiles of F47161T and F64183T contained phosphatidylethanolamine, two aminolipids, one glycolipid, one phosphoglycolipid and two unidentified polar lipids. Differential phenotypic and genotypic characteristics of the two strains indicated that the two strains should be classified as representing two novel species of the genus Aequorivita, for which the names Aequorivita vitellina sp. nov. and Aequorivita xiaoshiensis sp. nov. are proposed. The type strains are F47161T (=MCCC 1H00509T=KCTC 92017T) and F64183T (=MCCC 1H00507T=KCTC 92016T), respectively.

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“…The monomeric SoxB, which was defined as S-sulfosulfanyl-l-cysteine sulfohydrolas, interacted with the SoxYZ complex and was believed to function as a sulphate thiohydrolase [21]. Strain N1Y112 T and P. granulatum JCM 30316 T were incubated using marine broth (MB) prepared with each strains' optimum salinities and modified artificial seawater (0.67 % Na 2 S 2 O 3 , 0.48 % MgCl 2 , 0.12 % CaCl 2 , 0.07 % KCl and 0.02 % NaHCO 3 , all w/v [22]). The sulphate in the culture was detected by FeCl 3 and BaCl 2 solution at 0 and 7 days, and the results showed that sulphate produced from thiosulhate only in the culture medium of strain N1Y112 T (Fig.…”

Section: Genomic Analysesmentioning

confidence: 99%

Pontibacterium sinense sp. nov., a nitrate-reducing and thiosulphate-oxidizing bacterium, isolated from coastal sediment

Chen,

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, N1Y112T, was isolated from coastal sediment collected in Weihai, PR China. This Gram-stain-negative, facultatively anaerobic, motile rod-shaped bacterium exhibited the ability to oxidize thiosulphate to sulphate and reduce nitrate to ammonia through its Sox system and nitrate reduction pathway, respectively. The strain grew at 20–35 °C (optimum, 28 °C), pH 6.0–10.0 (optimum, pH 7.5) and in the presence of 1.0–5.0 % (w/v) NaCl (optimum, 3.0 %). Major fatty acids present in the strain included summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c), summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0. Its polar lipid profile consisted of one phosphatidylethanolamine, two unknown aminolipids, one aminophosphoglycolipid, one diphosphatidylglycerol, one phosphatidylglycerol, two unknown phospholipids and two unknown lipids. Strain N1Y112T contained ubiquinone-7 and ubiquinone-8 as isoprenoid quinones, with a genomic G+C content of 50.6 mol%. Based on phylogenetic analysis, strain N1Y112T clustered with Pontibacterium granulatum JCM 30316T being its closest relative at 97.1 % 16S rRNA gene sequence similarity. The average nucleotide identity and digital DNA–DNA hybridization values were 77.1 and 20.7 %, respectively, which suggest significant differences between genomes of N1Y112T and P. granulatum JCM 30316T. Based on the findings from its phenotypic, genotypic and phylogenetic analyses, N1Y112T is considered to represent a novel species of the genus Pontibacterium , for which the name Pontibacterium sinense sp. nov. is proposed. The type strain is N1Y112T (=KCTC 72927T=MCCC 1H00429T).

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Salibaculum halophilum gen. nov., sp. nov. and Salibaculum griseiflavum sp. nov., in the family Rhodobacteraceae

Cited by 17 publications

References 41 publications

Phenotypic and genotypic characterization of Marinobacterium weihaiense sp. nov. and Marinobacterium marinum sp. nov., isolated from marine sediment, and genomic properties of the genus Marinobacterium

Phenotypic and genotypic characterization of Marinobacterium weihaiense sp. nov. and Marinobacterium marinum sp. nov., isolated from marine sediment, and genomic properties of the genus Marinobacterium

Aequorivita vitellina sp. nov. and Aequorivita xiaoshiensis sp. nov., isolated from marine sediment

Pontibacterium sinense sp. nov., a nitrate-reducing and thiosulphate-oxidizing bacterium, isolated from coastal sediment

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