Salicola salis sp. nov., an extremely halophilic bacterium isolated from Ezzemoul sabkha in Algeria

Kharroub, Karima; Aguilera, Margarita; Quesada, Teresa; Morillo, José A.; Ramos‐Cormenzana, A.; Boulharouf, Abdrahmane; Monteoliva‐Sánchez, Mercedes

doi:10.1099/ijs.0.64482-0

Cited by 23 publications

(15 citation statements)

References 30 publications

(28 reference statements)

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“…We identified moderate halophiles of the genus Rhodovibrio and extremely halophilic Salicola salis , a non-pigmented bacterium originally isolated from a sabkha in Algeria (18) as well as strains of Salinibacter ruber . In addition, we obtained another group of isolates most closely related to Salinibacter ruber , but sharing only 93.5% 16S rRNA gene sequence similarity.…”

Section: Discussionmentioning

confidence: 99%

Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

et al. 2012

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Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5–4 × 106 cells mL−1) were similar to total cell abundance in the lake determined by DAPI direct count (3–4×107 cells mL−1). The proportion of Bacteria to Archaea in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most belonged to the genera Halorubrum (55%) and Haloarcula (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera Salinibacter (18.7%), Salicola (18.7%) and Rhodovibrio (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed 37 OTUs. The majority (63%) of these sequences were not related to any previously identified taxa. Within this sampling effort we most frequently retrieved phylotypes related to Halorhabdus (16% of archaeal sequences obtained) and Salinibacter (36% of bacterial sequences obtained). Other prokaryotic groups that were abundant included representatives of Haloquadratum, the anaerobic genera Halanaerobium and Halocella, purple sulfur bacteria of the genus Halorhodospira and Cyanobacteria.

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Section: Discussionmentioning

confidence: 99%

Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

et al. 2012

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“…Three strains (IC10, IC14 and IC15) were not phylogenetically assigned to the family Halobacteriaceae . Strain IC10, showing lipolytic and proteolytic activities, was closely related to Salicola species (Kharroub et al , 2006; Maturrano et al , 2006, respectively). The isolate IC14 showed lipolytic activity and its close relative was P. halophila (Sorokin & Tindall, 2006).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Salicola sp. âIC10, a lipase- and protease-producing extreme halophile

Moreno

Garcı́a²,

Ventosa

et al. 2009

FEMS Microbiology Ecology

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In order to explore the diversity of extreme halophiles able to produce different hydrolytic enzymes (amylase, protease, lipase and DNAse) in hypersaline habitats of South Spain, a screening program was performed. A total of 43 extreme halophiles showing hydrolytic activities have been isolated and characterized. The isolated strains were able to grow optimally in media with 15-20% (w/v) total salts and in most cases, growth was detected up to 30% (w/v) total salts. Most hydrolase producers were assigned to the family Halobacteriaceae, belonging to the genera Halorubrum (22 strains), Haloarcula (nine strains) and Halobacterium (nine strains), and three isolates were characterized as extremely halophilic bacteria (genera Salicola, Salinibacter and Pseudomonas). An extremely halophilic isolate, strain IC10, showing lipase and protease activities and identified as a Salicola strain of potential biotechnological interest, was further studied. The optimum growth conditions for this strain were 15-20% (w/v) NaCl, pH 8.0, and 37 degrees C. Zymographic analysis of strain IC10 detected the lipolytic activity in the intracellular fraction, showing the highest activity against p-nitrophenyl-butyrate as a substrate in a colorimetric assay, whereas the proteolytic activity was detected in the extracellular fraction. This protease degraded casein, gelatin, bovine serum albumin and egg albumin.

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“…Cellular morphology was determined by phase-contrast microscopy (Zeiss), and colony morphology was determined by a Leica M8 stereomicroscope using cultures grown on Nutrient Broth (NB) agar (2%) plates for 24 h at 50°C. Gram staining was carried out according to Dussault (1955). Unless otherwise stated, the strain was characterised using the modified methods of Gordon and Pang (1973).…”

Section: Bacterial Strain and Cell Cultivationmentioning

confidence: 99%

Production, purification and characterisation of thermostable metallo-protease from newly isolated Bacillus sp. KG5

Ahmetoglu¹,

Bekler²,

Acer³

et al. 2015

Eurasia J Biosci

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Background: Due to the importance of microbial proteases in biotechnological applications, a number of microorganisms are being explored. The production, purification and characterisation of extracellular metallo-proteases by producing Bacillus sp. KG5 was studied. Material and Methods: Bacterial strain KG5 was isolated from Kös (Bingöl) hot spring. The strain KG5 was identified by morphological, physiological, biochemical and 16S rRNA gene sequencing. The effects of various parameters on protease production, such as time, temperature, pH, carbon and nitrogen sources and CaCl2 were studied. The enzyme was purified by ammonium sulphate precipitation and Sephadex G-75 gel permeation chromatography. Molecular weight was calculated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and zymographic analysis. The effects of some metal ions, chelators and inhibitors on enzyme activity were determined. Results: The optimum temperature, pH and incubation period for protease production were 40-45°C, 7.0 and 24 h, respectively. It was determined that the best nitrogen sources were yeast extract and urea, while the best carbon sources were lactose and galactose. However, glucose as a source of carbon was found to inhibit the production of the enzyme. The maximum enzyme production was increased in the presence of CaCl2. The molecular weight of purified enzyme was found to be approximately 48 kDa. It was found that the enzyme was fully stable in the presence of 2 mM CaCl2 at 50°C after 120 min. Purified protease was significantly activated by Ca 2+ and Mg 2+ , while it was greatly inhibited by Cu 2+ , Zn 2+ , Hg 2+ and SDS as well as by the metal ion chelators ethylenediaminetetraacetic (EDTA) and 1,10-phenanthroline. Phenylmethylsulfonyl fluoride (PMSF) had a little effect on the enzyme. Conclusions: Our findings suggest the potential of this isolate for protease production and that this enzyme may be suitable for biotechnological applications.

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Salicola salis sp. nov., an extremely halophilic bacterium isolated from Ezzemoul sabkha in Algeria

Cited by 23 publications

References 30 publications

Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

Characterization of Salicola sp. âIC10, a lipase- and protease-producing extreme halophile

Production, purification and characterisation of thermostable metallo-protease from newly isolated Bacillus sp. KG5

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Salicola salis sp. nov., an extremely halophilic bacterium isolated from Ezzemoul sabkha in Algeria

Cited by 23 publications

References 30 publications

Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran

Characterization of Salicola sp. âIC10, a lipase- and protease-producing extreme halophile

Production, purification and characterisation of thermostable metallo-protease from newly isolated Bacillus sp. KG5

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Characterization of Salicola sp. âIC10, a lipase- and protease-producing extreme halophile