Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid

Fukami, Hiroyuki; Asakura, Tomiko; Honda, Hiroshi; Abe, Keiko; Shimomura, Koichiro; Yamakawa, Takashi

doi:10.1093/pcp/pcf119

Cited by 35 publications

(24 citation statements)

References 25 publications

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“…The A. belladonna SAMT gene was isolated and characterized in the cultured hairy roots of A. belladonna after exogenous SA had been administered (Fukami et al 2002). In the present study, AbSAMT1 was induced by exogenous SA in A. belladonna plants as well as in cultured hairy roots (Figure 1).…”

Section: Discussionsupporting

confidence: 52%

“…A. belladonna SAMT1 (AbSAMT1) (GenBank accession number: AB049752) showed that the expression of the AbSAMT1 gene was activated when exogenous SA was added to a medium containing cultured hairy roots of A. belladonna (Fukami et al 2002). In the present study, AbSAMT1 was expressed when A. belladonna was treated with exogenous SA, pathogens or 1,2-benzisothiazole-1,1-dioxide (BIT), by activating SAR as the chemical inducer.…”

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confidence: 66%

“…It has been shown that some SAMT genes are induced in defense responses under biotic and/or abiotic stresses (Fukami et al 2002;Negre et al 2002;Chen et al 2003;Ament et al 2004;Xu et al 2006;Koo et al 2007). The A. thaliana BSMT1 (AtBSMT1) gene was induced in leaves by treatment with alamethicin, a channel-forming peptide that mimics the effect of pathogen attack, Plutella sylostella herbivory, uprooting, physical wounding and methyl jasmonate (MeJA), but it was not induced by SA .…”

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confidence: 99%

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Biotic and abiotic stresses induce AbSAMT1, encoding S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase, in Atropa belladonna

Kwon¹,

Hamada²,

Matsuyama³

et al. 2009

Plant Biotechnology

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Section: Discussionsupporting

confidence: 52%

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confidence: 66%

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confidence: 99%

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Biotic and abiotic stresses induce AbSAMT1, encoding S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase, in Atropa belladonna

Kwon¹,

Hamada²,

Matsuyama³

et al. 2009

Plant Biotechnology

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“…6) suggests that SAMT-expression is dependent on JA, similar to MeJA-inducible AtBSMT1 expression in Arabidopsis (Chen et al, 2003). However, in Atropa belladonna root cultures, SAMT appeared SA-inducible (Fukami et al, 2002). We were able to confirm the JA-dependency, since treatment of def-1 with JA resulted in enhanced spider miteinduced SAMT transcription (Fig.…”

Section: Ja Is Involved In Mesa and Tmtt Emissionmentioning

confidence: 54%

Jasmonic Acid Is a Key Regulator of Spider Mite-Induced Volatile Terpenoid and Methyl Salicylate Emission in Tomato

et al. 2004

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The tomato (Lycopersicon esculentum) mutant def-1, which is deficient in induced jasmonic acid (JA) accumulation upon wounding or herbivory, was used to study the role of JA in the direct and indirect defense responses to phytophagous mites (Tetranychus urticae). In contrast to earlier reports, spider mites laid as many eggs and caused as much damage on def-1 as on wild-type plants, even though def-1 lacked induction of proteinase inhibitor activity. However, the hatching-rate of eggs on def-1 was significantly higher, suggesting that JA-dependent direct defenses enhanced egg mortality or increased the time needed for embryonic development. As to gene expression, def-1 had lower levels of JA-related transcripts but higher levels of salicylic acid (SA) related transcripts after 1 d of spider mite infestation. Furthermore, the indirect defense response was absent in def-1, since the five typical spider mite-induced tomato-volatiles (methyl salicylate [MeSA], 4,8,12-trimethyltrideca-1,3,7,11-tetraene [TMTT], linalool, trans-nerolidol, and trans-b-ocimene) were not induced and the predatory mite Phytoseiulus persimilis did not discriminate between infested and uninfested def-1 tomatoes as it did with wild-type tomatoes. Similarly, the expression of the MeSA biosynthetic gene salicylic acid methyltransferase (SAMT) was induced by spider mites in wild type but not in def-1. Exogenous application of JA to def-1 induced the accumulation of SAMT and putative geranylgeranyl diphosphate synthase transcripts and restored MeSA-and TMTT-emission upon herbivory. JA is therefore necessary to induce the enzymatic conversion of SA into MeSA. We conclude that JA is essential for establishing the spider mite-induced indirect defense response in tomato.

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“…The snapdragon BA methyltransferase (BAMT) showed activity with BA but not with SA, although the K m -value for the physiological substrate BA, 1.1 mM , is considerably higher than the reported K m -values for SAMTs using SA, which range from 24 to 83 mM. So far, no SAMT or BAMT enzymatic activities in these species have been detected in organs other than¯owers (a putative SAMT, with a poorly characterized substrate speci®city, was reported to be induced in hairy root cultures of Atropa belladonna upon SA treatment; Fukami et al (2002)). Speci®cally, neither the expression of SAMT and BAMT could be detected in leaves under normal conditions, nor was the expression of SAMT and BAMT induced in leaves by various treatments, including administration of SA or methyljasmonate (MeJA; Negre et al, 2002;Ross, 2002).…”

Section: Introductionmentioning

confidence: 73%

An Arabidopsis thaliana gene for methylsalicylate biosynthesis, identified by a biochemical genomics approach, has a role in defense

et al. 2003

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SummaryEmission of methylsalicylate (MeSA), and occasionally of methylbenzoate (MeBA), from Arabidopsis thaliana leaves was detected following the application of some forms of both biotic and abiotic stresses to the plant. Maximal emission of MeSA was observed following alamethicin treatment of leaves. A gene (AtBSMT1) encoding a protein with both benzoic acid (BA) and salicylic acid (SA) carboxyl methyltransferase activities was identi®ed using a biochemical genomics approach. Its ortholog (AlBSMT1) in A. lyrata, a close relative of A. thaliana, was also isolated. The AtBSMT1 protein utilizes SA more ef®ciently than BA, whereas AlBSMT1 catalyzes the methylation of SA less effectively than that of BA. The AtBSMT1 and AlBSMT1 genes showed expression in leaves under normal growth conditions and were more highly expressed in the¯owers. In A. thaliana leaves, the expression of AtBSMT1 was induced by alamethicin, Plutella xylostella herbivory, uprooting, physical wounding, and methyl jasmonate. SA was not an effective inducer. Using a b-glucuronidase (GUS) reporter approach, the promoter activity of AtBSMT1 was localized to the sepals of¯owers, and also to leaf trichomes and hydathodes. Upon thrip damage to leaves, AtBSMT1 promoter activity was induced speci®cally around the lesions.

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Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid

Cited by 35 publications

References 25 publications

Biotic and abiotic stresses induce AbSAMT1, encoding S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase, in Atropa belladonna

Biotic and abiotic stresses induce AbSAMT1, encoding S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase, in Atropa belladonna

Jasmonic Acid Is a Key Regulator of Spider Mite-Induced Volatile Terpenoid and Methyl Salicylate Emission in Tomato

An Arabidopsis thaliana gene for methylsalicylate biosynthesis, identified by a biochemical genomics approach, has a role in defense

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