Salicylic Acid Inhibits Synthesis of Proteinase Inhibitors in Tomato Leaves Induced by Systemin and Jasmonic Acid

Abstract

Salicylic acid (SA) and acetylsalicylic acid (ASA), previously shown to inhibit proteinase inhibitor synthesis induced by wound- Willmitzer [19931 Planta 191: 123-128), are shown here t o be potent inhibitors of systemin-induced and jasmonic acid (JA)-induced synthesis of proteinase inhibitor mRNAs and proteins. The inhibition by SA and ASA of proteinase inhibitor synthesis induced by systemin and JA, as well as by wounding and oligosaccharide elicitors, provides further evidence that both oligosaccharide and polypeptide inducer molecules utilize the octadecanoid pathway to signal the activation of proteinase inhibitor genes. Tomato (Lycopersicon esculentum) leaves were pulse labeled with [35S]methionine, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the inhibitory effects of SA are shown to be specific for the synthesis of a small number of JA-inducible proteins that includes the proteinase inhibitors. Previous results have shown that SA inhibits the conversion of 1 3s-hydroperoxy linolenic acid t o 12-0x0-phytodienoic acid, thereby inhibiting the signaling pathway by blocking synthesis of JA. Here we report that the inhibition of synthesis of proteinase inhibitor proteins and mRNAs by SA in both light and darkness also occurs at a step i n the signal transduction pathway, after JA synthesis but preceding transcription of the inhibitor genes.

Arabidopsis MYC Transcription Factors Are the Target of Hormonal Salicylic Acid/Jasmonic Acid Cross Talk in Response to Pieris brassicae Egg Extract

“…In contrast, antagonism of the SA signaling pathway on the JA pathway has been extensively studied (Caarls et al, 2015). SA suppressed JA-dependent defense responses in tomato (Solanum lycopersicum; Doares et al, 1995), tobacco (Nicotiana tabacum; Niki et al, 1998), and Arabidopsis (Arabidopsis thaliana; Spoel et al, 2003). Consequently, stimulation of the SA pathway diminished plant response to a necrotrophic fungus (Spoel et al, 2007) and a generalist herbivore (Cipollini et al, 2004).…”

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis

“…6F). Inhibition of JA and/or ethylene signaling by SA was also observed earlier in plants suffering biotic and abiotic stresses (Pena-Cortes et al, 1993;Doares et al, 1995;Berrocal-Lobo et al, 2002). Therefore, it appears that in the selenite-treated vtc1 mutant, an increase in SA production inhibited JA and ethylene signaling, leading to impaired Se resistance.…”

Regulation of the Wound‐Induced Myrosinase‐Associated Protein Transcript in Brassica Napus Plants

“…This notion is further strengthened by the fact that SA down-regulated both the developmentally expressed and the induced transcripts. However, SA seems capable of affecting several putative gene-regulatory pathways (Dempsey and Klessig, 1995;Doares et al, 1995;Leslie and Romani, 1988;Pena-Cortes et al, 1993;Wildon et al, 1989).…”

“…Some of the signals have been associated with the regulation of responses toward both wounding, herbivores and pathogens, as well as towards other aspects of the life of the plant. Furthermore, there appears to be a crosstalk between different signaling pathways with both synergistic effects such as ethylene/MeJA and SA/MeJA (Xu et al, 1994) and SNethylene (Lawton et al, 1994) and antagonistic effects such as wounding/SA (Pena-Cortes et al, 1993) and JAlSA (Doares et al, 1995). Such cross-talk might represent a mecha-nism to generate specificity the signal transduction.…”