Salicylic and jasmonic acid pathways are necessary for defence against <i><scp>D</scp>ickeya solani</i> as revealed by a novel method for Blackleg disease screening of <i>in vitro</i> grown potato

Burra, Dharani Dhar; Mühlenbock, Per; Andréasson, Erik

doi:10.1111/plb.12339

Cited by 22 publications

(16 citation statements)

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“…Jasmonates and SA are essential phytohormones for plant development and survival, and play an important role in plant resistance to fungal diseases [50, 51]. COI1 is a key component of the JA-mediated signal transduction pathway, leading to the dissociation between JAZ and the transcription factor MYC2, which could induce defense-related genes.…”

Section: Discussionmentioning

confidence: 99%

Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae

et al. 2017

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BackgroundSunflower Verticillium wilt (SVW) is a vascular disease caused by root infection with Verticillium dahliae (V. dahlia). It is a serious threat to the yield and quality of sunflower. However, chemical and agronomic measures for controlling this disease are not effective. The selection of more resistant genotypes is a desirable strategy to reduce contamination. A deeper knowledge of the molecular mechanisms and genetic basis underlying sunflower Verticillium wilt is necessary to accelerate breeding progress.ResultsAn RNA-Seq approach was used to perform global transcriptome profiling on the roots of resistant (S18) and susceptible (P77) sunflower genotypes infected with V. dahlia. Different pairwise transcriptome comparisons were examined over a time course (6, 12 and 24 h, and 2, 3, 5 and 10 d post inoculation). In RD, SD and D datasets, 1231 genes were associated with SVW resistance in a genotype-common transcriptional pattern. Moreover, 759 and 511 genes were directly related to SVW resistance in the resistant and susceptible genotypes, respectively, in a genotype-specific transcriptional pattern. Most of the genes were demonstrated to participate in plant defense responses; these genes included peroxidase (POD), glutathione peroxidase, aquaporin PIP, chitinase, L-ascorbate oxidase, and LRR receptors. For the up-regulated genotype-specific differentially expressed genes (DEGs) in the resistant genotype, higher average fold-changes were observed in the resistant genotype compared to those in the susceptible genotype. An inverse effect was observed in the down-regulated genotype-specific DEGs in the resistant genotype. KEGG analyses showed that 98, 112 and 52 genes were classified into plant hormone signal transduction, plant-pathogen interaction and flavonoid biosynthesis categories, respectively. Many of these genes, such as CNGC, RBOH, FLS2, JAZ, MYC2 NPR1 and TGA, regulate crucial points in defense-related pathway and may contribute to V. dahliae resistance in sunflower.ConclusionsThe transcriptome profiling results provided a clearer understanding of the transcripts associated with the crosstalk between sunflower and V. dahliae. The results identified several differentially expressed unigenes involved in the hyper sensitive response (HR) and the salicylic acid (SA)/jasmonic acid (JA)-mediated signal transduction pathway for resistance against V. dahliae. These results are useful for screening resistant sunflower genotypes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3386-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae

et al. 2017

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“…In addition, the authors found that the JA signalling pathway is also involved in susceptibility since the JA insensitive jai1 and spr2 mutants were more resistant, while prosystemin overexpressing plants, that have increased systemin, which triggers JA biosynthesis, were more susceptible to A. alternata infection (Jia et al 2013 ). In potato, both SA and JA pathways were shown to be required for foliar and tuber defence against the necrotrophic bacterial pathogen Dickeya solani, one of the causal agents of blackleg disease (Burra et al 2015 ). Additionally, it is becoming increasingly evident that not only the JA and SA signalling pathways are important in the host defence against necrotrophs, but that the plant hormones abscisic acid (ABA) and auxin (IAA) can also modulate host defence against necrotrophs (Mengiste 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani

et al. 2020

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Key Message Using disease bioassays and transcriptomic analysis we show that intact SA-signalling is required for potato defences against the necrotrophic fungal pathogen Alternaria solani. Abstract Early blight, caused by the necrotrophic fungus Alternaria solani, is an increasing problem in potato cultivation. Studies of the molecular components defining defence responses to A. solani in potato are limited. Here, we investigate plant defence signalling with a focus on salicylic acid (SA) and jasmonic acid (JA) pathways in response to A. solani. Our bioassays revealed that SA is necessary to restrict pathogen growth and early blight symptom development in both potato foliage and tubers. This result is in contrast to the documented minimal role of SA in resistance of Arabidopsis thaliana against necrotrophic pathogens. We also present transcriptomic analysis with 36 arrays of A. solani inoculated SA-deficient, JA-insensitive, and wild type plant lines. A greater number of genes are differentially expressed in the SA-deficient mutant plant line compared to the wild type and JA-insensitive line. In wild type plants, genes encoding metal ion transporters, such as copper, iron and zinc transporters were upregulated and transferase-encoding genes, for example UDP-glucoronosyltransferase and Serine-glyoxylate transferase, were downregulated. The SA-deficient plants show upregulation of genes enriched in GO terms related to oxidoreductase activity, respiratory chain and other mitochondrial-related processes. Pathogenesis-related genes, such as genes encoding chitinases and PR1, are upregulated in both the SA-deficient and wild type plants, but not in the JA-insensitive mutants. The combination of our bioassays and the transcriptomic analysis indicate that intact SA signalling, and not JA signalling, is required for potato defences against the necrotrophic pathogen A. solani.

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“…Table 1 contain information about number of plants or tubers that were investigated in each phenotype study, and under what conditions it was carried out. Dickeya scoring of in vitro plants was done according to [33]. P. infestans resistance on tubers (tuber blight) were assessed as described in [21], and the Phytophthora resistance data on leaves (late blight) is described in Lenman et al 2016.…”

Section: Plant Growth Phenotyping Sample Collection and Preparationmentioning

confidence: 99%

Linking crop traits to transcriptome differences in a progeny population of tetraploid potato

et al. 2020

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Background: Potato is the third most consumed crop in the world. Breeding for traits such as yield, product quality and pathogen resistance are main priorities. Identifying molecular signatures of these and other important traits is important in future breeding efforts. In this study, a progeny population from a cross between a breeding line, SW93-1015, and a cultivar, Désirée, was studied by trait analysis and RNA-seq in order to develop understanding of segregating traits at the molecular level and identify transcripts with expressional correlation to these traits. Transcript markers with predictive value for field performance applicable under controlled environments would be of great value for plant breeding.Results: A total of 34 progeny lines from SW93-1015 and Désirée were phenotyped for 17 different traits in a field in Nordic climate conditions and controlled climate settings. A master transcriptome was constructed with all 34 progeny lines and the parents through a de novo assembly of RNA-seq reads. Gene expression data obtained in a controlled environment from the 34 lines was correlated to traits by different similarity indices, including Pearson and Spearman, as well as DUO, which calculates the co-occurrence between high and low values for gene expression and trait. Our study linked transcripts to traits such as yield, growth rate, high laying tubers, late and tuber blight, tuber greening and early flowering. We found several transcripts associated to late blight resistance and transcripts encoding receptors were associated to Dickeya solani susceptibility. Transcript levels of a UBXdomain protein was negatively associated to yield and a GLABRA2 expression modulator was negatively associated to growth rate. Conclusion: In our study, we identify 100's of transcripts, putatively linked based on expression with 17 traits of potato, representing both well-known and novel associations. This approach can be used to link the transcriptome to traits. We explore the possibility of associating the level of transcript expression from controlled, optimal environments to traits in a progeny population with different methods introducing the application of DUO for the first time on transcriptome data. We verify the expression pattern for five of the putative transcript markers in another progeny population.

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Salicylic and jasmonic acid pathways are necessary for defence against Dickeya solani as revealed by a novel method for Blackleg disease screening of in vitro grown potato

Cited by 22 publications

References 43 publications

Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae

Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae

Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani

Linking crop traits to transcriptome differences in a progeny population of tetraploid potato

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