Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK‐Dependent NLRP3 Inflammasome Regulation

Ma, Weidong; Wang, Ziyuan; Zhao, Yan; Wang, Qibin; Zhang, Yonghong; Pan, Lei; Lu, Wei; Yan, Shan; Zhou, Jun; Li, Xiaojiao; Yu, Wenjun; Zhong, Yaoxin; Chen, Li; Zheng, Tao

doi:10.1155/2021/6614574

Cited by 24 publications

(16 citation statements)

References 49 publications

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“…Consistent with previous reports [9,11,29], SAL directly inhibits the proliferation of tumor cells and induces tumor cell apoptosis, suggesting that SAL is an ideal drug for antitumor therapy. Moreover, SAL directly inhibits the proliferation of tumor cells [9,11,29] and regulates the activity and function of immune cells in the tumor microenvironment. In the mouse model of lung injury, SAL also regulated the secretion of in ammatory factors and the number of neutrophils and macrophages, further protecting LPS-induced lung injury [35].…”

Section: Discussionsupporting

confidence: 91%

“…SAL on anticancer decreases proliferation and induces apoptosis in A549 cells through its ability to inhibit oxidative stress and p38 [10]. It also suppresses the proliferation and migration of human lung cancer cells through AMPK-dependent NLRP3 in ammasome regulation [11]. Previous studies have shown that SAL regulates the immune response [12].…”

Section: Introductionmentioning

confidence: 98%

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Salidroside regulates tumor microenvironment of non-small cell lung cancer via Hsp70/Stub1/Foxp3 pathway in Tregs

Liu

Yue

Meng

et al. 2023

Preprint

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Background: The treatment of non-small cell lung cancer (NSCLC) is challenging due to immune tolerance and evasion. Salidroside (SAL) is an extract in traditional Chinese medicine and has a potential antitumor effect. However, the mechanism of SAL in regulating the immunological microenvironment of NSCLC is yet to be clarified. Methods: The mouse model with Lewis lung cancer cell line (3LL) in C57BL/6 mice was established. And then, the percentage of tumor-infiltrating T cell subsets including Treg was detected in tumor-bearing mice with or without SAL treatment. In vitro, the effect of SAL on the expression of IL-10, Foxp3 and Stub1 and the function of Treg were detected by flow cytometry. Network pharmacology prediction and molecular docking software were used to predict the target of SAL and intermolecular interaction. Furthermore, the effect of SAL on the expression of Hsp70 and the co-localization of Stub1-Foxp3 in Treg was confirmed by flow cytometry and confocal laser microscopy. Finally, Hsp70 inhibitor was used to verify the above molecular expression. Results: We discovered that SAL treatment inhibits the growth of tumor cells by decreasing the percentage of tumor-infiltrated CD4+Foxp3+T cells. SAL treatment downregulates the expression of Foxp3 in Tregs, but increases the expression of Stub1, an E3 ubiquitination ligase upstream of Foxp3, and the expression of Hsp70. Inhibiting the expression of Hsp70 reverses the inhibition of SAL on Foxp3 and disrupts the colocalization of Stub1 and Foxp3 in the nucleus of Tregs. Conclusions: SAL inhibits tumor growth by regulating the Hsp70/stub1/Foxp3 pathway in Treg to suppress the function of Treg. It is a new mechanism of SAL for antitumor therapy.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 98%

Salidroside regulates tumor microenvironment of non-small cell lung cancer via Hsp70/Stub1/Foxp3 pathway in Tregs

Liu

Yue

Meng

et al. 2023

Preprint

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“…Its molecular formula and molecular weight are C 14 H 20 O 7 and 300.3, respectively [ 19 ]. Salidroside is documented to have a wide range of pharmacological effects, such as antioxidative, anti-inflammatory, antihypoxic, antifatigue, antidepressive, antiaging, and antitumor properties [ 20 – 26 ]. Various studies have confirmed that salidroside possesses ameliorative effect on ischemia-reperfusion injury in the heart, brain, liver, and spinal cord [ 27 – 30 ].…”

Section: Introductionmentioning

confidence: 99%

Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

Wei

Huang

Qin

2022

Oxidative Medicine and Cellular Longevity

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Testicular torsion-detorsion results in testicular ischemia-reperfusion injury, which is associated with overgeneration of reactive oxygen species. Salidroside, a major bioactive ingredient extracted from Rhodiola rosea, has strong antioxidant activity. The purpose of this study was to examine the effect of salidroside on testicular ischemia-reperfusion injury. Sixty rats were randomly separated into 3 experimental groups: group A = sham-operated control; group B = testicular ischemia-reperfusion; and group C = testicular ischemia-reperfusion treated with salidroside. The rats in the sham-operated control group received all surgical procedures except testicular torsion-detorsion. The testicular ischemia-reperfusion group underwent 2 hours of left testicular torsion followed by detorsion. The rats in the salidroside-treated group received the same surgical procedure as in testicular ischemia-reperfusion group, but salidroside was injected intraperitoneally at reperfusion. Testicular malondialdehyde content (a reliable index of reactive oxygen species) and protein expression of superoxide dismutase and catalase which are primary antioxidant enzymes in testes were measured at 4 hours after reperfusion. Testicular spermatogenesis was evaluated at 3 months after reperfusion. The malondialdehyde content increased significantly, while superoxide dismutase and catalase protein expression and testicular spermatogenesis reduced significantly in ipsilateral testes of testicular ischemia-reperfusion group, as compared with sham-operated control group. Therapy with salidroside significantly reduced malondialdehyde content and significantly enhanced superoxide dismutase and catalase protein expression and spermatogenesis in ipsilateral testes, as compared with testicular ischemia-reperfusion group. The present findings indicate that treatment with salidroside ameliorates testicular ischemia-reperfusion injury by reducing reactive oxygen species level by upregulating superoxide dismutase and catalase protein expression.

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“…In addition, many studies have shown that salidroside has anti-cancer, anti-apoptosis, anti-virus, anti-depression, anti-osteoporosis and other effects [53][54][55][56][57].…”

Section: Anti-telomere Shorteningmentioning

confidence: 99%

Progress in the Protective Mechanism of Salidroside in Chronic Obstructive Pulmonary Disease

Ruan¹,

Hu²

2023

IJCEMR

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Chronic obstructive pulmonary disease (COPD) is a heterogeneous lung disease that is persistent, repeatedly deteriorating airway obstruction due to respiratory abnormalities (bubuchitis, bronchiolitis) and/or alveolar abnormalities (emphysema). Salidroside, a widespread natural phenolic secondary metabolite found in Rhododiola, has several pharmacological effects. Relevant studies have shown that salidroside has protective effects in anti-diabetes, anti-cancer, anti-aging, heart and nerve protection, etc. Due to the limitations of clinical drug use in COPD, salidroside has multiple functions and low side effects, and its protective effect and mechanism of action on COPD have attracted increasing attention. By collecting relevant papers published in recent years, reviewed in the literature against oxidative stress, regulation of protease/anti-protease imbalance, anti-inflammatory, anti-bronchiolar and interstitial fibrosis, anti-telomere shortening, expounds the protective effect and mechanism of its effect in COPD, and provide reference for its clinical application. The clinical and experimental data are not sufficient, and the role and mechanism of salidroside need to be further studied.

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Salidroside Suppresses the Proliferation and Migration of Human Lung Cancer Cells through AMPK‐Dependent NLRP3 Inflammasome Regulation

Cited by 24 publications

References 49 publications

Salidroside regulates tumor microenvironment of non-small cell lung cancer via Hsp70/Stub1/Foxp3 pathway in Tregs

Salidroside regulates tumor microenvironment of non-small cell lung cancer via Hsp70/Stub1/Foxp3 pathway in Tregs

Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

Progress in the Protective Mechanism of Salidroside in Chronic Obstructive Pulmonary Disease

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